Effects of heavy oil in the developing spotted halibut, Verasper variegatus     

Murakami Y  Kitamura S  Nakayama K  Matsuoka S  Sakaguchi H 《Marine pollution bulletin》2008,57(6-12):524-528

It is well known that heavy oil (HO) on the sea surface causes serious problems in the aquatic environment. In particular, some species of teleosts which develop on the sea surface are thought to be affected by the HO which flows out from tankers or coastal industry. However, the toxicological effects of HO are not fully understood. We performed exposure experiments using the Pleuronectiformean fish, spotted halibut (Verasper variegatus), which is an important fishery resource in Japan. In course of the development, HO-exposed embryos showed remarkable delay in developmental processes including somite formation. We further observed abnormal development of the head morphology. Notably, treated embryos had relatively small eyes and craniofacial structures. These findings strongly suggest that HO seriously affects the cell proliferation and differentiation of the embryo. In addition, HO-exposed embryos showed abnormal neuronal development. We also performed the exposure in the larval stage. Treatment of post-hatching larvae with HO resulted in significantly greater mortality compared with controls. Through these observations, we finally conclude that HO is strongly toxic to halibut in their early life stages.  相似文献   

Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil     

Nakayama K  Kitamura S  Murakami Y  Song JY  Jung SJ  Oh MJ  Iwata H  Tanabe S 《Marine pollution bulletin》2008,57(6-12):445-452

Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19-20 degrees C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins.  相似文献   

Effects of heavy oil in the developing spotted halibut, Verasper variegatus     

Yasunori Murakami  Shin-Ichi Kitamura  Kei Nakayama  Satoru Matsuoka  Hideo Sakaguchi 《Marine pollution bulletin》2008,57(6-12):524

It is well known that heavy oil (HO) on the sea surface causes serious problems in the aquatic environment. In particular, some species of teleosts which develop on the sea surface are thought to be affected by the HO which flows out from tankers or coastal industry. However, the toxicological effects of HO are not fully understood. We performed exposure experiments using the Pleuronectiformean fish, spotted halibut (Verasper variegatus), which is an important fishery resource in Japan. In course of the development, HO-exposed embryos showed remarkable delay in developmental processes including somite formation. We further observed abnormal development of the head morphology. Notably, treated embryos had relatively small eyes and craniofacial structures. These findings strongly suggest that HO seriously affects the cell proliferation and differentiation of the embryo. In addition, HO-exposed embryos showed abnormal neuronal development. We also performed the exposure in the larval stage. Treatment of post-hatching larvae with HO resulted in significantly greater mortality compared with controls. Through these observations, we finally conclude that HO is strongly toxic to halibut in their early life stages.  相似文献   

THE GALATIA,KANSAS, CHONDRITE     

W.R. Van Schmus  Klaus Kei  D.E. Lange  G.H. Conrad 《Meteoritics & planetary science》1978,13(2):267-273

The Galatia meteorite was found in August, 1971, approximately 7 km ENE of Galatia, Barton County, Kansas (98° 53′W., 38° 39.5′N). The single stone weighed 23.9 kg and is partially weathered. Olivine (Fa_24.9) and pyroxene (Fs_20.9) compositions indicate L-group classification, and textural observations indicate that the stone is of petrologic type 6. Galatia is similar in many respects to the Otis L6 chondrite (found 20 km to the west), but it does not have the brecciated structure of Otis and, thus, it is not part of the same fall.  相似文献   

Heat-Mitigation Effects of Irrigated Rice-Paddy Fields Under Changing Atmospheric Carbon Dioxide Based on a Coupled Atmosphere and Crop Energy-Balance Model     

Ikawa  Hiroki  Kuwagata  Tsuneo  Haginoya  Shigenori  Ishigooka  Yasushi  Ono  Keisuke  Maruyama  Atsushi  Sakai  Hidemitsu  Fukuoka  Minehiko  Yoshimoto  Mayumi  Ishida  Sachinobu  Chen  Charles P.  Hasegawa  Toshihiro  Watanabe  Tsutomu 《Boundary-Layer Meteorology》2021,179(3):447-476

Boundary-Layer Meteorology - Known as the heat-mitigation effect, irrigated rice-paddy fields distribute a large fraction of their received energy to the latent heat during the growing season. The...  相似文献   

Historical record of heavy metal pollution deduced by lead isotope ratios in core sediments from the Osaka Bay,Japan     

Takahiro Hosono  Chih-Chieh Su  Kei Okamura  Makoto Taniguchi 《Journal of Geochemical Exploration》2010

Three marine sediment cores from Osaka Bay were analyzed for ²¹⁰Pb geochronology, heavy metal concentrations (Zn, Cu, and Pb) and stable lead isotope ratios (²⁰⁶Pb/²⁰⁷Pb) in order to reconstruct high-resolution heavy metal pollution history from 1900–2006. Anthropogenic metal accumulation in sediments peaked in 1970 in agreement with the high economic growth period in Japan. The comparison of temporal patterns of ²⁰⁶Pb/²⁰⁷Pb ratio with other areas of Japan suggested that the heavy metals, imported from several different countries during the periods of economic growth (1955 to 1973), are the main pollution source for the country. For the period 1970–2006, the sediment data reflect the result of stricter environmental regulations applied after the late 1960s. However, heavy metal concentrations in the surface sediments are still elevated to levels several times higher than the levels at the bottoms of the cores. Additionally, the lead isotope ratio does not show significant change after the 1980s. Secondary heavy metal pollution through the mixing of deeper polluted sediment appears to be the likely reason for the deterioration of present time submarine sediment environments. In conclusion, this study has demonstrated that it is difficult to recover over a period of several years the benthic quality of a bay, once it is heavily polluted.  相似文献   

Source distribution of acoustic emissions during an in-situ direct shear test: Implications for an analog model of seismogenic faulting in an inhomogeneous rock mass     

Tsuyoshi Ishida  Tadashi Kanagawa  Yuji Kanaori 《Engineering Geology》2010,110(3-4):66-76

We monitored acoustic emission (AE) events during an in-situ direct shear test on a specimen composed of a slate-dominant alternation of slate and sandstone, measuring 0.5 m long, 0.5 m wide and 0.2 m high. The test was conducted in a survey tunnel for an underground powerhouse in central Japan. The AE epicenters located on a fractured plane are compared with the locations of joints and a loosening seam, the height distribution of the fractured plane, and the horizontal movement of the test block prior to failure. We conclude that an initially intact region of rock bounded by the joints and the seam is fractured, generating the AE. Considering these results in connection with asperity models of seismogenic faulting for a subduction-zone earthquake, the significant contrast of stress conditions derived from the geological inhomogeneity and the uneven fractured plane is analogous to that due to subducted seamounts and horst-graben structures on a subducted oceanic plate. For an inland earthquake, the intact regions on an expected shear plane can be considered to be a portion of the fault asperity that causes strong ground motion, while the weakened portion can be considered to correspond to a region of aseismic creep. Consequently, large-scale inhomogeneous rock fracturing experiments such as the in-situ direct shear test may provide useful insights as analog models of seismogenic faulting. Furthermore, understanding of inhomogeneous rock-mass fracturing obtained from such experiments will not only contribute to a better understanding of the mechanism of earthquakes but also provide valuable knowledge for AE monitoring applications in rock engineering, such as the predictions of rockbursts in mines and the monitoring of fractures around large underground chambers.  相似文献   

Heavy oil exposure induces high moralities in virus carrier Japanese flounder Paralichthys olivaceus     

Song JY  Nakayama K  Murakami Y  Kitamura S 《Marine pollution bulletin》2011,63(5-12):362-365

The relationship between chemical exposure and disease outbreak in fish has not been fully defined due to the limitations of experimental systems (model fish and pathogens). Therefore, we constructed a system using the Japanese flounder, Paralichthys olivaceus, and viral haemorrhagic septicemia virus (VHSV), and evaluated it by heavy oil (HO) exposure. The fish were exposed to HO at 0.3, 0.03, 0.003, and 0 g/L following VHSV infection at doses of 10(2.5) or 10(3.5) tissue culture infectious dose (TCID)50/fish. As a result, groups given the dual stressors showed more than 90% mortality. Although VHSV infection at 10(2.5) and 10(3.5) TCID50/fish without HO exposure also induced high mortality, at 68.8% and 81.3%, respectively, HO exposure induced faster and higher mortality in the virus carrier fish, indicating that chemical stressors raise the risk of disease outbreak in fish. The experimental system established in this study could be useful for chemical risk assessment.  相似文献   

Potential of superelastic Cu–Al–Mn alloy bars for seismic applications     

Y. Araki  T. Endo  T. Omori  Y. Sutou  Y. Koetaka  R. Kainuma  K. Ishida 《地震工程与结构动力学》2011,40(1):107-115

We present the results of the quasi‐static cyclic tensile tests of Cu–Al–Mn shape memory alloy (SMA) bars of 4 and 8 mm diameters to examine their superelasticity and other mechanical properties closely related to seismic applications. The present Cu–Al–Mn SMA bars have achieved the recovery strains of over 8% and the fracture strains of over 17%. Low‐cycle fatigue was observed in neither of the bars. The mechanical properties obtained from the test, along with the lower material cost and higher machinability than Ni–Ti SMAs, demonstrate the high potential of the present Cu–Al–Mn SMA bars to be used in seismic applications. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

Pressure-induced structural evolution of pyrite-type SiO<Subscript>2</Subscript>     

Yasuhiro Kuwayama  Kei Hirose  Nagayoshi Sata  Yasuo Ohishi 《Physics and Chemistry of Minerals》2011,38(8):591-597

The equation of state and pressure-induced structural evolution of pyrite-type SiO₂ have been investigated based on synchrotron X-ray diffraction measurements in a diamond-anvil cell. The Rietveld refinement revealed that the oxygen coordinate x of pyrite-type SiO₂ increases with increasing pressure. The SiO₆ coordination polyhedra of pyrite-type SiO₂ is less compressible than the unit cell, and the increase in x induces a rotation of the SiO₆ coordination polyhedra to fill the blank space in the unit cell. Thus, the volume reduction in pyrite-type SiO₂ is achieved mainly by the rotation of the SiO₆ polyhedra, rather than by the compression of the SiO₆ polyhedra. In addition, the increase in x with increasing pressure enhances a distortion of the coordination polyhedra of pyrite-type SiO₂, implying that pyrite-type SiO₂ is not likely to transform into a fluorite-type structure at higher pressures.  相似文献