Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences   总被引：7，自引：0，他引：7  

Lee SK  Wang HZ  Law SH  Wu RS  Kong RY 《Marine pollution bulletin》2002,44(5):412-420

Vibrios are widespread in the marine environment and a few pathogenic species are known to be commonly associated with outbreaks of diarrheal diseases in humans due to the consumption of raw or improperly cooked seafood. However, there are also many Vibrio species which are potentially pathogenic to vertebrate and invertebrate aquatic animals, and of which little is known. In an attempt to develop rapid PCR detection methods for these latter class of vibrios, we have examined the 16S-23S intergenic spacers (IGSs) of 10 lesser-known Vibrio species and successfully developed species-specific primers for eight of them--Vibrio costicola, V. diazotrophicus, V. fluvialis, V. nigripulchritudo, V. proteolyticus, V. salmonicida, V. splendidus and V. tubiashii. The IGS amplicons were amplified using primers complementary to conserved regions of the 16S and 23S rRNA genes, and cloned into plasmid vectors and sequenced. Analysis of the IGS sequences showed that 37 ribosomal RNA (rrn) operons representing seven different IGS types have been cloned from the 10 vibrios. The three IGS types--IGS(0), IGS(IA) and IGS(Glu)--were the most prevalent forms detected. Multiple alignment of representative sequences of these three IGS types from different Vibrio species revealed several domains of high sequence variability, which were used to design species-specific primers for PCR. The specificity of the primers were evaluated using total DNA prepared from different Vibrio species and bacterial genera. The results showed that the PCR method can be used to reliably detect eight of the 10 Vibrio species in marine waters in this study.  相似文献   

The Reykjanes Ridge: structure and tectonics of a hot-spot-influenced, slow-spreading ridge, from multibeam bathymetry, gravity and magnetic investigations     

R.C Searle  J.A Keeton  R.B Owens  R.S White  R Mecklenburgh  B Parsons  S.M Lee   《Earth and Planetary Science Letters》1998,160(3-4):463-478

We report a comprehensive morphological, gravity and magnetic survey of the oblique- and slow-spreading Reykjanes Ridge near the Iceland mantle plume. The survey extends from 57.9°N to 62.1°N and from the spreading axis to between 30 km (3 Ma) and 100 km (10 Ma) off-axis; it includes 100 km of one arm of a diachronous ‘V-shaped' or ‘chevron' ridge. Observed isochrons are extremely linear and 28° oblique to the spreading normal with no significant offsets. Along-axis there are ubiquitous, en-echelon axial volcanic ridges (AVRs), sub-normal to the spreading direction, with average spacing of 14 km and overlap of about one third of their lengths. Relict AVRs occur off-axis, but are most obvious where there has been least axial faulting, suggesting that elsewhere they are rapidly eroded tectonically. AVRs maintain similar plan views but have reduced heights nearer Iceland. They are flanked by normal faults sub-parallel to the ridge axis, the innermost of which occur slightly closer to the axis towards Iceland, suggesting a gradual reduction of the effective lithospheric thickness there. Generally, the amplitude of faulting decreases towards Iceland. We interpret this pattern of AVRs and faults as the response of the lithosphere to oblique spreading, as suggested by theory and physical modelling. An axial, 10–15 km wide zone of high acoustic backscatter marks the most recent volcanic activity. The zone's width is independent of the presence of a median valley, so axial volcanism is not primarily delimited by median valley walls, but is probably controlled by the lateral distance that the oblique AVRs can propagate into off-axis lithosphere. The mantle Bouguer anomaly (MBA) exhibits little mid- to short-wavelength variation above a few milliGals, and along-axis variations are small compared with other parts of the Mid-Atlantic Ridge. Nevertheless, there are small axial deeps and MBA highs spaced some 130 km along-axis that may represent subdued third-order segment boundaries. They lack coherent off-axis traces and cannot be linked to Oligocene fracture zones on the ridge flanks. The surveyed chevron ridge is morphologically discontinuous, comprising several parallel bands of closely spaced, elevated blocks. These reflect the surrounding tectonic fabric but have higher fault scarps. There is no evidence for off-axis volcanism or greater abundance of seamounts on the chevron. Free-air gravity over it is greater than expected from the observed bathymetry, suggesting compensation via regional rather than pointwise isostasy. Most of the observed variation along the ridge can be ascribed to varying distance from the mantle plume, reflecting changes in mantle temperature and consequently in crustal thickness and lithospheric strength. However, a second-order variation is superimposed. In particular, between 59°30′N and 61°30′N there is a minimum of large-scale faulting and crustal magnetisation, maximum density of seamounts, and maximum axial free-air gravity high. To the north the scale of faulting increases slightly, seamounts are less common, and there is a relative axial free-air low. We interpret the 59°30′N to 61°30′N region as where the latest chevron ridge intersects the Reykjanes Ridge axis, and suggest that the morphological changes that culminate there reflect a local temperature high associated with a transient pulse of high plume output at its apex.  相似文献   

Modeling the Fate and Transport of Household Chemicals in Septic Systems     

Sijin Lee  Drew C. McAvoy  James Szydlik  Jerald L. Schnoor 《Ground water》1998,36(1):123-132

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Assessment of the geological and hydrogeological environment at a site of government-level conflict in Incheon, Korea     

J. E. Lee  K. K. Lee 《Environmental Geology》1998,35(4):278-286

 The natural environment of an environmental conflict site in Incheon, Korea was assessed with a focus on the hydrogeological environment. Residents had made claims against a company because of health problems, including skin tumors. More specifically, the residents suspected that their drinking groundwater had been contaminated by glass fibers which had caused the health problems. Air, soil, and groundwater samples were analyzed to estimate whether environmental pollution could have caused the problems claimed by the residents. No specific evidence was found from this study to support the groundwater contamination by glass fibers, but groundwater contamination by leaked fuel oils was confirmed in the course of evaluating the glass fiber problem. Received: 16 May 1997 · Accepted: 18 August 1997  相似文献   

On the polarization of Hα scattered by neutral hydrogen in active galactic nuclei     

Hee-Won Lee  & Jong-Hyeun Yun 《Monthly notices of the Royal Astronomical Society》1998,301(1):193-202

Raman scattering by atomic hydrogen converts the UV continuum around Lyβ into optical continuum around Hα, and the basic atomic physics has been discussed in several works on symbiotic stars. We propose that the same process may operate in active galactic nuclei (AGN) and calculate the linear polarization of the broad emission lines Raman-scattered by high-column neutral hydrogen component. The conversion efficiency of the Raman scattering process is discussed and the expected scattered flux is computed using the spectral energy distribution of an AGN given by a typical power law. The high-column H  i component in AGN is suggested by many observations, encompassing the radio through UV and X-ray ranges.   When neutral hydrogen component with a column density ∼10²² cm⁻² is present around the active nucleus, it is found that the scattered Hα is characterized by a very broad width ∼20 000 km s⁻¹ and that the strength of the polarized flux is comparable to that of the electron-scattered flux expected from a conventional unified model of narrow-line AGN. The width of the scattered flux is mainly determined by the column density of the neutral scatterers where the total scattering optical depth becomes of order unity. The asymmetry in the Raman scattering cross-section around Lyβ introduces red asymmetric polarized profiles around Hα. The effects of the blended Lyβ and O  vi 1034 doublet are also investigated.   We briefly discuss the spectropolarimetric observations performed on the Seyfert galaxy IRAS 110548-1131 and the narrow line radio galaxy Cyg A. Several predictions regarding the scattering by the high-column neutral hydrogen component in AGN are discussed.  相似文献   

Heterolocalism: an alternative model of the sociospatial behaviour of immigrant ethnic communities   总被引：2，自引：0，他引：2  

Zelinsky W  Lee BA 《International journal of population geography : IJPG》1998,4(4):281-298

"This paper evaluates critically the applicability of the well-known assimilation and pluralist models to the contemporary ethnic landscape of the U.S.... We then consider an alternative model, labelled heterolocalism, which suggests that members of certain newly arrived groups may be able to sustain their identity as an ethnic community despite immediate or rapid spatial dispersion. The applicability of the heterolocal perspective to non-metropolitan and transnational phenomena is evaluated in subsequent sections of the paper."  相似文献   

Observations and origin of Rayleigh-wave amplitude anomalies   总被引：1，自引：0，他引：1  

S. van der Lee 《Geophysical Journal International》1998,135(2):691-699

This is a report of observations of amplitude anomalies of fundamental-mode Rayleigh waves ( R 1) between periods of 17 and 100  s. The anomalies are with respect to amplitudes predicted by Rayleigh-wave excitation for a reference earth model and catalogued centroid earthquake source parameters, such as are used in large-scale waveform inversions. The observations indicate that the amplitude anomalies are consistent for nearby recordings of the same event, while there is no obvious relation between the observed anomalies and the paths travelled by the waves. This is in contrast to Rayleigh-wave phase anomalies, which are consistent for similar propagation paths, and hence form the input in many inversions for along-path structure. The observations in this paper show that a similar inversion of intermediate-period amplitude anomalies for along- and near-path structure is not warranted without eliminating source effects, since the amplitude anomalies are dominated by scattering off near-source earth structure and by possible uncertainties in the source parameters. Sensitivity kernels that take the coupling between the moment tensor and displacement field into account demonstrate that Rayleigh-wave amplitude sensitivity is largest near the source. This report argues that the interaction between source-radiated Rayleigh waves and near-source earth structure may not be ignored in amplitude inversion procedures.  相似文献   

Effect of hydrologic management on marsh surface sediment deposition in coastal Louisiana     

Denise J. Reed  Nina De Luca  A. Lee Foote 《Estuaries and Coasts》1997,20(2):301-311

High rates of coastal land loss in Louisiana have prompted efforts to maintain or restore coastal wetland habitats, and structural management of marsh hydrology is one of a number of approaches that has been adopted. The aim of this study was to determine the effect of hydrologic management measures on marsh-surface sediment deposition in the Mississippi deltaic plain. Four impoundments, ranging in size from 50 ha to 177 ha and similarly sized control sites were included in the study. At each site, marsh-surface sediment traps were collected approximately biweekly for 3.5 yr to measure changes in sediment deposition. There was no significant difference in sediment deposition between any of the impoundment and control sites during the premanagement period. The results show no significant difference in marsh-surface sediment deposition between management year 1992–1993 and management year 1994–1995, but management year 1993–1994 had significantly lower sediment deposition than either the first or the last year of the study. Management year 1992–1993 showed the highest sediment deposition when it was assessed across all basins, areas, and sites, and this high rate of deposition is accounted for by the impact of Hurricane Andrew. For all management years there were significant differences between impoundment and control sites, with control sites showing higher rates of marsh surface sediment deposition than impoundments. No clear pattern was identified concerning the influence of various types of hydrologic management on rates of sediment deposition. This reduction in sediment deposition indicated that the areas under management in this study are receiving insufficient inorganic sediment deposition to keep pace with sea-level rise.  相似文献   

Decomposition of saltmeadow cordgrass (Spartina patens) in Louisiana coastal marshes     

A. Lee Foote  Kathleen A. Reynolds 《Estuaries and Coasts》1997,20(3):579-588

In Louisiana, plant production rates and associated decomposition rates may be important in offsetting high rates of land loss and subsidence in organic marsh soils. Decomposition ofSpartina patens shoot and leaf material was studied by using litter bags in mesohaline marshes in the Barataria and Terrebonne basins of coastal Louisiana.Spartina patens decomposed very slowly with an average decay constant of 0.0007, and approximately 50% of the material remained after 2 years in the field. Material at the Barataria site decomposed faster than did Terrebonne material with trend differences apparent during the first 150 days. This difference might be explained by the higher content of phosphorus in the Barataria material or a flooding period experienced by the Barataria bags during their first 10 days of deployment. Nitrogen and carbon content of the plant material studied did not differ between the two basins. We detected no consistent significant differences in decomposition above, at, or below sediment/water level. BecauseS. patens is the dominant plant in these marshes, and because it is so slow to decompose, we believe thatS. patens shoots are an important addition to vertical accretion and, therefore, marsh elevation.  相似文献   

Effects of alternative cloud radiation parameterizations in a general circulation model     

Wan-Ho Lee  Richard C. J. Somerville 《Annales Geophysicae》1996,14(1):107-114

Using the National Center for Atmospheric Research (NCAR) general circulation model (CCM2), a suite of alternative cloud radiation parameterizations has been tested. Our methodology relies on perpetual July integrations driven by ±2 K sea surface temperature forcing. The tested parameterizations include relative humidity based clouds and versions of schemes involving a prognostic cloud water budget. We are especially interested in testing the effect of cloud optical thickness feedbacks on global climate sensitivity. All schemes exhibit negative cloud radiation feedbacks, i.e., cloud moderates the global warming. However, these negative net cloud radiation feedbacks consist of quite different shortwave and longwave components between a scheme with interactive cloud radiative properties and several schemes with specified cloud water paths. An increase in cloud water content in the warmer climate leads to optically thicker middle- and low-level clouds and in turn negative shortwave feedbacks for the interactive radiative scheme, while a decrease in cloud amount leads to a positive shortwave feedback for the other schemes. For the longwave feedbacks, a decrease in high effective cloudiness for the schemes without interactive radiative properties leads to a negative feedback, while no distinct changes in effective high cloudiness and the resulting feedback are exhibited for the scheme with interactive radiative properties. The resulting magnitude of negative net cloud radiation feed-back is largest for the scheme with interactive radiative properties. Even though the simulated values of cloud radiative forcing for the present climate using this method differ most from the observational data, the approach shows great promise for the future.  相似文献