Fixing the wheel the carpetbaggers broke     

Martin M  Lam PK  Richardson BJ 《Marine pollution bulletin》2003,46(7):918-920

  相似文献   

Exposure and time dependent DNA strand breakage in hepatopancreas of green-lipped mussels (Perna viridis) exposed to Aroclor 1254, and mixtures of B[a]P and Aroclor 1254     

Siu WH  Hung CL  Wong HL  Richardson BJ  Lam PK 《Marine pollution bulletin》2003,46(10):1285-1293

Green-lipped mussels (Perna viridis) were exposed to Aroclor 1254 (0.5, 5 and 50 microgl(-1)) and a mixture of benzo[a]pyrene (B[a]P) and Aroclor 1254 (0.3+0.5 and 3+5 microgl(-1)) for 12 days. On day 0, 1, 3, 6 and 12, the levels of DNA strand breaks in the mussel hepatopancreas were monitored using an alkaline unwinding assay. The results were compared to the findings of a previous study in which the levels of DNA strand breakage in the same species were measured following exposure to various concentrations of B[a]P (0.3, 3 and 30 microgl(-1)). The results indicated that Aroclor 1254 at a concentration 相似文献   

Harmonisation of polychlorinated biphenyl (PCB) analyses for ecotoxicological interpretations of southeast Asian environmental media: what's the problem?     

Martin M  Richardson BJ  Lam PK 《Marine pollution bulletin》2003,46(2):159-170

  相似文献   

Uptake and depuration of PAHs and chlorinated pesticides by semi-permeable membrane devices (SPMDs) and green-lipped mussels (Perna viridis)     

Richardson BJ  Tse ES  De Luca-Abbott SB  Martin M  Lam PK 《Marine pollution bulletin》2005,51(8-12):975-993

Semi-batch seawater experiments were conducted to follow the uptake and release of selected PAHs (anthracene, fluorathene, pyrene and B[a]P) and organochlorine pesticides (-HCH, aldrin, dieldrin, p,p′-DDT) in semi-permeable membrane devices (SPMDs) and green-lipped mussels (Perna viridis). Mathematical models were applied to describe the uptake and elimination curves of the contaminants for SPMDs, and kinetic parameters, such as uptake rate constants, and equilibrium triolein/water partitioning coefficients were calculated. SPMD data showed a good fit to estimate rate constant and partition coefficient equations, but only those contaminants which partitioned mainly in the dissolved phase (-HCH and dieldrin) were well explained for mussels. Poor conformity of the other contaminants indicated mussels uptake by routes other than diffusion, such as ingestion of algae. An apparent equilibrium state was only noted for -HCH in mussels. Aldrin was not detected in mussels in the first few days of exposure, indicating potential metabolism of this compound. B[a]P was not detected in the triolein of SPMDs, which suggests that the membrane may act as a reservoir. Loss of spiked B[a]P from the triolein was evident in a depuration experiment, which may indicate transfer to the membrane. Rate constants for mussels were higher than those for SPMDs, but the reverse was true for partition coefficients. Overall, mussels and SPMDs had similar uptake rates for all compounds in this study, excluding p,p′-DDT and dieldrin. Contaminant elimination took place more rapidly in mussels, implying that SPMDs are better candidates for detecting episodic discharge of organic contaminants.  相似文献   

Identification and characterization of a new degradation product of Irgarol-1051 in mercuric chloride-catalyzed hydrolysis reaction and in coastal waters     

Lam KH  Lam MH  Lam PK  Qian T  Cai Z  Yu H  Cheung RY 《Marine pollution bulletin》2004,49(4):361-367

  相似文献   

Near-surface attenuation modelling based on rock shear-wave velocity profile   总被引：1，自引：0，他引：1  

A.M. Chandler  N.T.K. Lam  H.H. Tsang 《Soil Dynamics and Earthquake Engineering》2006,26(11):1004-1014

In a previous study published in this journal, the authors developed a comprehensive methodology for modelling the shear wave velocity profile in crustal rock, for purposes of seismic hazard assessment. The derived shear wave velocity profile was used to estimate the amplification and attenuation mechanisms in the transmission of seismic waves. The ability to conduct seismic hazard assessments in regions of low and moderate seismicity is greatly enhanced by this new modelling approach, given that developing a local attenuation model based on curve-fitting strong motion data is generally not feasible under such conditions. This paper reports a follow-up study conducted to evaluate the significance of near-surface attenuation in bedrock (as distinct from attenuation in unconsolidated soft soil sediments). The κ parameter is used to characterize the extent of this attenuation mechanism. Empirical correlations of κ with two forms of near-surface shear wave velocity parameter in crustal rock have been developed, employing information obtained from global sources in conjunction with that from local studies. The resulting development of two simple equations to predict median values of κ as functions of readily available shear wave velocity parameters represents the key outcome of this study. Applications of the proposed empirical approaches to determine κ have been provided, taking Hong Kong and Melbourne as case studies to illustrate different aspects of the proposed methodology. Consistency between the results obtained by the two recommended approaches has thereby been demonstrated.  相似文献   

Simple models for estimating period‐shift and damping in soil     

Hing‐Ho Tsang  Adrian M. Chandler  Nelson T. K. Lam 《地震工程与结构动力学》2006,35(15):1925-1947

This study describes the development of a simple, heuristic manual calculation procedure for estimating the site period‐shift factor and soil damping ratio, with appropriate considerations for the level of shaking, impedance contrast between soil and bedrock interface and the plasticity of the soil layers. Essentially, the analogy of a building shear‐frame has been used to represent the seismic response behaviour of a soil column. The proposed procedure has been verified by comparing the predictions with results obtained directly from non‐linear shear wave analyses of soil column models. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Regional and local factors in attenuation modelling: Hong Kong case study   总被引：1，自引：0，他引：1  

A.M. Chandler  N.T.K. Lam  H.H. Tsang 《Journal of Asian Earth Sciences》2006,27(6):892-906

Seismic attenuation behaviour is controlled by a large number of wave modification mechanisms. The characteristics of some of these mechanisms are specific to a local area, whilst the remainder can be generalised to the entire seismic region. Factors representing these mechanisms are often not resolved. A new attenuation modelling approach is demonstrated in this paper (using Hong Kong as a case study), to evaluate individual regional and local wave modification factors. Shear wave velocity (SWV) information for the four prevalent geological formations found in Hong Kong was first obtained: (a) at shallow depths from instrumented boreholes; (b) at depths of up to 100–200 m from measurements using the Microtremor SPatial Auto-Correlation (SPAC) technique; (c) at depths of up to 1.5 km from the monitoring of quarry blasts; and (d) at depths from 1.5 to 8 km in the hard basement rock layers from results of seismological refraction surveys. The upper-crust amplification factor calculated from the four modelled rock SWV profiles was then combined with predicted attenuation parameters to determine the upper-crust modification factor (filter function) incorporating the local wave modification characteristics associated with Hong Kong geological formations. Such functions may then be combined with the regional attenuation characteristics in that part of the South China region. A seismic attenuation model was developed by combining the upper-crust modification factor with the regional source function of intra-plate earthquakes, based on stochastic simulations. The ground shaking model developed from the presented methodology is supported by the comparison with macro-seismic data of seven historical earthquake events affecting Hong Kong.  相似文献   

Field validation of antioxidant enzyme biomarkers in mussels (Perna viridis) and clams (Ruditapes philippinarum) transplanted in Hong Kong coastal waters   总被引：1，自引：1，他引：1  

Sharon B. De Luca-Abbott  Bruce J. Richardson  Katherine E. McClellan  Gene J. Zheng  Michael Martin  Paul K.S. Lam 《Marine pollution bulletin》2005,51(8-12):694

Green-lipped mussels, Perna viridis, and Manila clams, Ruditapes philippinarum were sourced from “clean” sites in the Hong Kong region, depurated in a laboratory using uncontaminated filtered seawater for 8 days, and transplanted to a suspected gradient of chemically polluted sites in Hong Kong. After 14- and 28-days of field exposure, several antioxidant parameters including glutathione S transferase (GST), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH) were quantified in gill and hepatopancreas tissues. Whole body tissue concentrations of polycyclic aromatic hydrocarbons (PAHs), petroleum hydrocarbons (PHCs), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs) were determined in pooled site samples. Chemical analyses indicated that: (a) clams had higher levels of PAHs, PHCs, DDTs and PCBs, whereas mussels had higher hexachlorocyclohexane (HCHs) and there was no difference between species for dieldrin and remaining OCs; (b) Kat O should not be continued as a “clean” reference site for Hong Kong, because of the levels of contaminants measured and (c) PAH concentrations in the current survey were similar to those previously measured. Toxicological conclusions were: (a) antioxidant responses were different between species; (b) CAT and GST have highest utility in clams for field use in Hong Kong, whereas CAT in both gill and hepatopancreas tissue showed most potential in mussels; (c) significant induction of antioxidant responses over day 0 (excluding GPx in both tissues, and GST in mussel hepatic tissue); (d) groups of contaminants do not consistently induce antioxidant responses and (e) organochlorines and PCBs correlated significantly with CAT and GST in clam hepatopancreas and with CAT in mussel gill and hepatic tissue. Multivariate statistical techniques indicated little relationship between the site patterns for antioxidant responses and the contaminant gradients identified in body burden analysis.  相似文献   

Okadaic acid, a causative toxin of diarrhetic shellfish poisoning, in green-lipped mussels Perna viridis from Hong Kong fish culture zones: Method development and monitoring     

Kylie C.Y. Mak  H. Yu  M.C. Choi  Xiaoyun Shen  Michael H.W. Lam  M. Martin  Rudolf S.S. Wu  Patsy S. Wong  Bruce J. Richardson  Paul K.S. Lam   《Marine pollution bulletin》2005,51(8-12):1010

Green-lipped mussels (Perna viridis) were collected from seven fish culture zones (FCZs) in Hong Kong and analyzed for okadaic acid (OA). A conventional HPLC method was modified by incorporating a proteinase K digestion step. Results suggest that a higher recovery (2.5 times higher) of OA was obtained from spiked samples after the addition of 1.08 mg proteinase K in comparison with samples incubated without the proteolytic enzyme. For the hepatopancreas (HP) of individual field-collected mussels, the additional digestion step can enhance OA extraction by 3.1 times. Spatial and temporal variations in OA concentrations in the mussels from various FCZs were investigated. The highest concentration of OA in mussel HP samples was 1164.9 ng/g HP wet wt. With respect to OA concentrations in whole mussel tissues from seven sites and four seasonal samplings, the concentrations were between 70.0 and 131.0 ng/g wet wt., which did not exceed the generally recognized international regulatory criteria (>200 ng/g) for OA.  相似文献