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11.
Samples of marine benthic invertebrates collected from two sites in the Antarctic have been analysed for both aliphatic and aromatic hydrocarbons in order to establish baseline concentrations for some classes of hydrocarbons.Samples from Signy Island, a pristine site, contained low concentrations of the hydrocarbons determined, whereas those from King Edward Cove, South Georgia, contained significantly higher concentrations. King Edward Cove has a known history of pollution from whaling operations.Platt & Mackie (1979) have suggested that the hydrocarbons in sediments from King Edward Cove are a result of the world-wide dissemination of the pyrolysis products of fossil fuels. Our work suggests, in contrast, that the hydrocarbons in the benthos are derived from local sources. 相似文献
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R.J. Law 《Marine pollution bulletin》1978,9(11):293-296
Following the wreck of the AMOCO CADIZ on 16 March 1978, samples of water, sediment and oil were collected between April and June from the Brittany coast and western English Channel and were analysed for petroleum hydrocarbons by means of fluorescence spectroscopy, gas-liquid chromatography and gas chromatography-mass spectrometry. 相似文献
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The response of the electric and magnetic field variations over the San Juan Bay region of Vancouver Island is studied using a scaled laboratory analogue model. The laboratory frequencies simulate periods of 10 and 100 s in the geophysical problem. The model results indicate that, for both E- and H-polarization of the source field, induced current in the ocean is deflected around Cape Flattery and channelled into the Juan de Fuca Strait. With increasing period, the proportion of current channelled into the Strait decreases sharply. Induced current in the strait is also funnelled into San Juan Bay, a finger-shaped bay ca. 4 km wide and 7.5 km long, for both polarizations of the source field. The effect of the Bay on the field response is confined to the local region, within approximately 6 km of the centre of the Bay. Good agreement between field station and analogue model Hz results was obtained for the San Juan Bay. The behaviour of the Parkinson arrow for these two stations is examined with the aid of the analogue model results. 相似文献
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The solar brightness temperature was measured at frequencies of 19.0, 21.0, 22.2, 23.5, and 25.5 GHz on 8 days in February and March, 1966. The observed average brightness temperatures were 10 800, 10 900, 11 000, 10 700, and 9 800°K, respectively. The daily values were close to these averages.Presently at the University of Maryland. 相似文献
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Although Escherichia coli is widely distributed in the marine environment, only a small percentage are pathogenic to humans. Nonetheless, the widespread occurrence of waterborne infections of E. coli origin in humans has become one of the major health problems worldwide. To date, several types of enterovirulent E. coli have been recognized as the aetiologic agents of various gastrointestinal infections in humans. The most commonly encountered are those belonging to the enterotoxigenic (ETEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) subtypes. In order to better determine the health risks that are associated with exposure to some of these specific subtypes, we have developed a very sensitive multiplex PCR system for the rapid detection and typing of ETEC, EHEC and possibly EPEC strains of E. coli in the aquatic environment. The target genes chosen for this investigation included: the PHO-A housekeeping gene (present in all E. coli); the LT1, LT2 and ST1 genes of ETEC; the VT1 and VT2 verotoxin, and EAE virulence genes of EHEC and EPEC, respectively. Six pairs of oligonucleotide primers were designed to simultaneously amplify internal fragments of these genes by multiplex PCR to generate PCR products that could be analysed and confirmed with relative ease by gel electrophoresis and HincII enzyme digestion. The results showed that the six sets of PCR primers were highly specific for their target genes and produced specific amplimers of the expected size from several control strains of E. coli – ATCC 35401 (LT1+/ST1+); SA53 (LT2+/VT2+); and O157 (VT1+/VT2+/EAE+). The detection sensitivity of the multiplex PCR system for the six target genes in an E. coli cell mixture was optimized and enhanced by preincubating serially diluted cells in Luria-Bertani broth for 6 h prior to PCR analysis. The results obtained indicated a detection sensitivity of 10° CFU (of each strain) per 100 μl reaction. Multiplex PCR analysis of seawater samples collected from four sewage-polluted sites in Hong Kong indicated the presence in all four samples of E. coli bacteria that were positive for LT1, ST1, VT1 and EAE virulence genes. Overall, the data indicated that the multiplex PCR system described in this study is a potentially very useful and powerful method for routine monitoring and risk assessment of water quality. 相似文献