Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin     

Suzan Pantaroto de Vasconcellos  Elaine Crespim  Georgiana Feitosa da Cruz  Diego Barbosa Senatore  Karen Christina Marques Simioni  Eugênio Vaz dos Santos Neto  Anita Jocelyne Marsaioli  Valéria Maia de Oliveira 《Organic Geochemistry》2009,40(5):574-588

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.  相似文献   

Correction to: Spatial distribution and estimation of rainfall trends and erosivity in the Epitácio Pessoa reservoir catchment,Paraíba,Brazil     

da Silva  Richarde Marques  Santos  Celso Augusto Guimarães  Silva  Jorge Flávio Casé Braga da Costa  Silva  Alexandro Medeiros  Brasil Neto  Reginaldo Moura 《Natural Hazards》2020,104(2):1953-1954

Natural Hazards - The article “Spatial distribution and estimation of rainfall trends and erosivity in the Epitácio Pessoa reservoir catchment, Paraíba, Brazil”, written by da...  相似文献   

Tectonic inversion assessed by integration of geological and geophysical data: The intracontinental Rio do Peixe Basin,NE Brazil     

David L. Vasconcelos  Fernando O. Marques  Francisco C. C. Nogueira  Yoe A. R. Perez  Francisco H. R. Bezerra  Rmulo C. Stohler  Jorge A. B. Souza 《Basin Research》2021,33(1):705-728

Common basin models assume that the post‐rift tectonic evolution of most basins is usually associated with tectonic quiescence. However, tectonic inversion during the post‐rift phase has been proposed for several sedimentary basins worldwide, but how and why it happens is still a matter of debate, especially in intracontinental settings where the lithosphere is old and thick. Here, we use geological and geophysical data from the Rio do Peixe Basin in NE Brazil to show evidence that intracontinental sedimentary basins can be tectonically inverted by far‐field compressive stresses acting on pre‐existing weakness zones of lithospheric‐scale where stresses can concentrate and inversion can occur. Geomorphological and field data combined with seismic reflection, gravimetric and borehole data show that: (a) inversion occurred along two main Precambrian lithospheric‐scale shear zones, the Patos (E‐W trending) and Portalegre (NE‐SW trending), which had already been reactivated as basin‐bounding faults during the earlier rift stage; (b) post‐rift reactivation affected (mostly) the original master normal faults with the largest rift displacements, and locally produced new reverse faults; (c) during contraction, deformation was partitioned between fault reactivation and buckling of the incompetent sediment pushed against the hard basement; (d) all these signs of inversion have been observed in the field and can be demonstrated on seismic reflection profiles; and (e) combined gravimetric and seismic data show that the main structures of the basin were followed by an inversion. These data are consistent with the operation of WSW‐ENE horizontal maximum compressive stress as a result of combined pushes of the Mid‐Atlantic Ridge (towards the W) and the Andes (towards the E), responsible for the post‐rift oblique inversion of normal faults inherited from the rift phase and formed with vertical maximum compressive stress.  相似文献   

Urban forest loss using a GIS-based approach and instruments for integrated urban planning:A case study of Jo(a)o Pessoa,Brazil     

Leandro Ismael de Azevedo LACERDA  José Augusto Ribeiro da SILVEIRA  Celso Augusto Guimar 《地理学报(英文版)》2021,31(10):1529-1552

Urban forests play an important role in the thermal comfort and overall life of local populations in large-and medium-sized cities.This study analyzes urban forest loss and maps land use and land cover (LULC) changes between 1991 and 2018 by evaluating the use of urban planning instruments for the mitigation of urban forest loss in Jo～o Pessoa,Brazil.For this purpose,satellite-derived LULC images from 1991,2006,2010 and 2018 and data on urban forest loss areas obtained using the Google Earth Engine were used.In addi-tion,this paper also discusses the instruments used for integrated urban planning,which are(a) the legal sector,responsibility and nature;(b) the urban expansion process;and (c) the elements of urban infrastructure.The results show a clear shift in land use in the study area.The major changes in LULC classes occurred in urban areas and herbaceous vegetation,while the greatest loss was in arboreal/shrub vegetation.Thus,an increase in the pressure to occupy zones intended for environmental preservation could be estimated.Our results showed similar accuracies with other studies and more spatial details.The characteristics of the patterns,traces,and hotspots of urban expansion and forest cover loss were explored.We highlighted the potential use of this proposed framework to be applied and validated in other parts of the world to help better understand and quantify various aspects of ur-ban-related problems such as urban forest loss mapping using instruments for integrated urban planning and low-cost approaches.  相似文献   

Dynamics of Microbiological Contamination at a Marine Recreational Site     

M.P Pires Coelho  M Eugnia Marques  J Carlos Roseiro 《Marine pollution bulletin》1999,38(12):59-1246

Levels of bacterial indicators of pollution are related with marine salinity and turbidity at both high tide (HT) and low tide (LT). The salinity varied from values around 26.9 ppm at the LT and 28.6 ppm at the high tide but affected total and faecal coliform (FC) estimates. Salinity readings of 25–30 ppm produced microbial counts below 10⁻² MPN/100 ml total coliforms (TCs) whereas salinity of 15–22 ppm produced a TC level of 4.6×10⁻⁴ MPN/100 ml. Turbidity peaks in the samples are accompanied by peaks of microbial contamination of the seawater indicating that the contamination is normally deposited at the marine sediment rather than in the water column. In fact, samples collected under heavy stormy weather, in which the water agitation resulted in turbidity values up to 68.3 NTU, produced maximum microbial counts.  相似文献   

An Application of HFE-D for Evaluating Sea Water Intrusion in Coastal Aquifers of Southern Vietnam     

Thi Thuy Van Le  Rungruang Lertsirivorakul  Tran Vuong Bui  Marcia K. Schulmeister 《Ground water》2020,58(6):1012-1022

The coastal aquifers and inland waters of the Long Xuyen Quadrangle and Ca Mau Peninsula of southern Vietnam have been significantly impacted by sea water intrusion (SI) as a result of recent anthropogenic activities. This study identified the evolution and spatial distribution of hydrochemical conditions in coastal aquifers at this region using Hydrochemical Facies Evolution Diagram (HFE-D) and Geographical Information System mapping. Hydraulic heads and water chemistry were measured at 31 observation wells in four layered aquifers during dry and rainy seasons in early (2005), and more recent (2016), stages of agricultural development. Hydrochemical facies associated with intrusion or freshening stages were mapped in each aquifer after assigning mixing index values to each facies. The position of groundwater freshening and SI phases differed in Holocene, Upper Pleistocene, Middle Pleistocene, and Lower Pleistocene aquifers. The geographic position of freshening and intrusion fronts differ in dry and rainy seasons, and shifted after 11 years of groundwater abstraction in all four aquifers. The spatial and temporal differences in hydrochemical facies distributions according to HFE-D reflect the relative impact of SI in the four aquifers. The study results provide a better understanding of the evolution of groundwater quality associated with SI in a peninsular coastal aquifer system, and highlight the need for improving groundwater quality and management in similar coastal regions.  相似文献   

A new U–Pb zircon age dating and palynological data from a Lower Permian section of the southernmost Paraná Basin,Brazil: Biochronostratigraphical and geochronological implications for Gondwanan correlations     

Ana Luisa Outa Mori  Paulo Alves de Souza  Juliana Charão Marques  Ricardo da Cunha Lopes 《Gondwana Research》2012,21(2-3):654-669

Index species useful for correlations with the International Stratigraphical Column are rare or absent in the Pennsylvanian–Permian strata of the Paraná Basin in Brazil, preventing accuracy in geochronologic assignments. Besides, absolute datings are very scarce in comparison with other Gondwana basins. This paper presents palynological data from an outcrop on the surroundings of the Candiota coal mine, southmost Brazil, from several levels of the Rio Bonito and Palermo formations. The presence of certain index species of spore–pollen allowed the recognition of two Permian palynozones: the Vittatina costabilis and the Lueckisporites virkkiae zones. Furthermore, U–Pb in zircons from a volcaniclastic level interbedded in the coal strata of the former unit was analyzed through LA-MC-ICP-MS method, providing a new absolute age dating of 281.4 ± 3.4 Ma (Cisuralian, Early Permian). This dating is assumed as the oldest occurrence of the L. virkkiae Zone in Paraná Basin, which contains index species that are widespread in other Gondwana basins. A well distributed surface boundary occurs in this section also, allowing local and regional correlations. These new biostratigraphical and geochronological data are integrated, in order to offer a deep analysis on the stratigraphical significance for correlations across the Occidental Gondwana.  相似文献   

Response of single benthic metrics and multi-metric methods to anthropogenic pressure gradients, in five distinct European coastal and transitional ecosystems   总被引：4，自引：0，他引：4  

Borja A  Barbone E  Basset A  Borgersen G  Brkljacic M  Elliott M  Garmendia JM  Marques JC  Mazik K  Muxika I  Magalhães Neto J  Norling K  Rodríguez JG  Rosati I  Rygg B  Teixeira H  Trayanova A 《Marine pollution bulletin》2011,62(3):499-513

In recent times many benthic indices have been proposed to assess the ecological quality of marine waters worldwide. In this study we compared single metrics and multi-metric methods to assess coastal and transitional benthic status along human pressure gradients in five distinct environments across Europe: Varna bay and lake (Bulgaria), Lesina lagoon (Italy), Mondego estuary (Portugal), Basque coast (Spain) and Oslofjord (Norway). Hence, 13 single metrics (abundance, number of taxa, and several diversity and sensitivity indices) and eight of the most common indices used within the European Water Framework Directive (WFD) for benthic assessment were selected: index of size spectra (ISS), Benthic assessment tool (BAT), Norwegian quality index (NQI), Multivariate AMBI (M-AMBI), Benthic quality index (BQI), (Benthic ecosystem quality index (BEQI), Benthic index based on taxonomic sufficiency (BITS), and infaunal quality index (IQI). Within each system, sampling sites were ordered in an increasing pressure gradient according to a preliminary classification based on professional judgement. The different indices are largely consistent in their response to pressure gradient, except in some particular cases (i.e. BITS, in all cases, or ISS when a low number of individuals is present). Inconsistencies between indicator responses were most pronounced in transitional waters (i.e. IQI, BEQI), highlighting the difficulties of the generic application of indicators to all marine, estuarine and lagoonal environments. However, some of the single (i.e. ecological groups approach, diversity, richness) and multi-metric methods (i.e. BAT, M-AMBI, NQI) were able to detect such gradients both in transitional and coastal environments, being these multi-metric methods more consistent in the detection than single indices. This study highlights the importance of survey design and good reference conditions for some indicators. The agreement observed between different methodologies and their ability to detect quality trends across distinct environments constitutes a promising result for the implementation of the WFD’s monitoring plans. Moreover, these results have management implications, regarding the dangers of misclassification, uncertainty in the assessment, use of conflicting indices, and testing and validation of indices.  相似文献   

Patterns of rare earth and other trace elements in different size fractions of clays of Campanian–Maastrichtian deposits from the Portuguese western margin (Aveiro and Taveiro Formations)     

Rosa Marques  M. Isabel Prudêncio  M. Isabel Dias  Fernando Rocha 《Chemie der Erde / Geochemistry》2011,71(4):337-347

The Upper Cretaceous (Campanian–Maastrichtian) Taveiro and Aveiro Formations belong to the northern sector of the Lusitanian basin (Portuguese western margin). The Taveiro Formation was deposited in alluvial fans, including mud flow beds, lakes and sinuous rivers. The Aveiro Formation was deposited in a flat region with low hydrodynamics channels, with the formation of a barrier island-tidal system. The reconstruction of this sedimentary basin may be difficult due to its complex architecture. This work aims a methodology to be used in the reconstruction of Cretaceous sedimentary environments of the Lusitanian basin, through the establishment of geochemical patterns of different size fractions of those deposits. Chemical analysis was performed by instrumental neutron activation analysis (INAA), and the mineralogical composition obtained by X-ray diffractometry (XRD). The whole rock and different size fractions (? ≥ 125 μm, 63–125 μm, 20–63 μm, 2–20 μm and ? < 2 μm) of selected samples were studied aiming the rare earth elements (REE) and other trace elements distribution and its correlation with the grain size and mineralogy.The results obtained for the studied Cretaceous sediments showed that REE patterns and other trace elements distribution in the different size fractions may be used as a tool to differentiate deposits within and between sedimentary formations. Within the Taveiro Formation, REE are concentrated in the silt fractions (20–63 μm and 2–20 μm) of the Reveles deposit, and in the clay size fraction of S. Pedro deposit, which is richer in kaolinite. The 1st transition elements, particularly Zn, are correlated with the presence of smectite in the clay fraction of Reveles deposit. In the sand and silt size fractions (>2 μm) of samples from Taveiro Formation correlations were found between: Ga and 1st transition elements, and phyllosilicates; Cs and mica; and Rb and Ba, and K–feldspars.The Bustos deposit (Aveiro Formation) samples are very fine-grained and with a high proportion of the fine silt fraction where REE are concentrated, especially the heavy ones. Incorporation of MREE, Co and U in carbonates of the coarser fraction appears to occur. The abundances of the 1st transition elements, Ga and As, are correlated with phyllosilicates.Significant differences were found in the trace elements patterns of the various analysed size fractions of the Lusitanian basin of Cretaceous sediments, between and even within formations, which can be used as a methodological approach for a fine paleogeographic reconstruction.  相似文献   

Differences in the neighborhood: Structural variations in the carapace of shore crabs Carcinus maenas (Decapoda: Portunidae)     

Allan T. Souza  Martina I. Ilarri  Joana Campos  João C. Marques  Irene Martins 《Estuarine, Coastal and Shelf Science》2011

The present study compared the carapace structure of Carcinus maenas in two nearby sites (2 km apart) within Minho estuary, submitted to different physicochemical and ecological conditions (water temperature, pH, crabs’ density and sex ratio). The carapace structure of the carapace and chelae of the crabs presented significant differences between sampling sites (t-test; p < 0.01). The SIMPER analysis revealed that the Weight/CW and Thickness/CW ratios explained all the dissimilarities found among sites. Overall, the male carapace was proportionally thicker at station 2 (t-test; p < 0.01), while the female carapace was proportionally thicker at station 1 (t-test; p < 0.001). A thicker carapace can be advantageous when competing for food or a sexual partner. We hypothetized that, since at station 2, the density of individuals was twice higher than at station 1, it is likely that agonistic encounters are more frequent, thus favouring a thicker carapace.  相似文献