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21.
We propose a novel pragmatic approach of in situ 15N and 13C isotope labelling of trees for subsequent litter decomposition and turnover studies under field conditions. Using this method the labelling of even large trees under natural conditions is possible and compared to tree labelling under artificial conditions in greenhouses the in situ approach is less expensive. 13C and 15N labelling were carried out simultaneously via photosynthesis by tree gassing with 13CO2 and by stem injection of 15NH415NO3. The aims of this study were: (i) to produce a sufficient quantity of labelled plant material for subsequent field incubation studies and (ii) to investigate the effectiveness and distribution of in situ 15N (15NH415NO3) and 13C (13CO2) labelling of Podocarpus falcatus, Croton macrostachys, Prunus africana and Cupressus lusitanica. The following targets need to be achieved: (i) Assuming almost natural litter fall conditions, enough labelled plant material must be produced in situ for the turnover experiment; (ii) intra-plant tracer enrichment shall be homogeneous; (iii) tracer enrichment should be comparable for different tree species; and (iv) tracer enrichment must be sufficient for subsequent litter turnover studies using the stable isotope approach. Our results clearly demonstrated that several kilograms of labelled plant material can be produced in situ. For many ecosystems, this amount is sufficient for a long term litter turnover experiment on a field scale under almost natural litter fall conditions. However, intra-plant label uptake of 13C and 15N was heterogeneous so that only leaves (litter) should be used for the turnover study. It could be shown that only a part of the labile C and N fraction in the leaves was labelled. Nevertheless, label uptake was sufficient for subsequent litter turnover studies.  相似文献   
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The ctenophore Mnemiopsis leidyi is an ecologically important predator in temperate coastal environments. Their populations fluctuate seasonally, serving as sinks of nutrients during periodic blooms, but as sources via excretion and during population collapse. Ctenophores were analyzed for elemental composition (C, N, and P) during 2008 and 2009 in Great South Bay, NY, USA. Salt-free weight percent C, N, and P correlated positively with ctenophore sizes and zooplankton prey abundances. Nitrogen and P were higher at the onset of blooms than during collapse when prey were substantially fewer. Ctenophores collected during average to high zooplankton densities had atomic ratios averaging C/N ~6:1 and C/P ~66:1, but became C- and P-depleted (C/N ~5:1, C/P ~128:1) with decreasing zooplankton. Incubations demonstrated rapid remineralization of ctenophore biomass (as NH4 +, HPO4 2?), following first order kinetics (e.g., k ~0.1–0.4 day?1) with enriched stoichiometric N and P fractionation relative to biomass under both oxic and anoxic conditions. Based on reported excretion rates, nutrient regeneration from excretion by active populations greatly exceeds nutrients remineralized during population crashes. To our knowledge, this is the first study documenting natural seasonal patterns in ctenophore elemental stoichiometry as a function of ctenophore size and prey availability.  相似文献   
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In February 2008, cruise P362/2 was undertaken aboard R/V Poseidon to the Giza and North Alex mud volcanoes (MVs) on the upper slope of the western Nile deep-sea fan. Emitted fluids were strongly depleted in chloride and rich in hydrocarbons, predominantly of thermogenic origin. In-situ sediment temperature measurements indicate extremely high and moderate levels of activity for the North Alex MV and Giza MV, respectively, and suggest rapid changes from dormant to active stages. Both the physical properties of core sediments (e.g., color and magnetic susceptibility), and their assemblages of micro- and nannofossils point to different sources for the two mud volcanoes. Biostratigraphic dating suggests source depths of 2,100–2,450 mbsf for the Giza MV and 1,150–1,550 mbsf for the North Alex MV. Very high temperatures of up to 70°C in shallow sediments at the North Alex MV can be explained only if the fluid source were warmer and deeper than the sediment source.  相似文献   
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The European Research Project AquaDEB (2007–2011, http://www.ifremer.fr/aquadeb/) is joining skills and expertise of some French and Dutch research institutes and universities to analyse the physiological flexibility of aquatic organisms and to link it to ecological and evolutionary processes within a common theoretical framework for quantitative bioenergetics [Kooijman, S.A.L.M., 2000. Dynamic energy and mass budgets in biological systems. Cambridge University Press, Cambridge]. The main scientific objectives in AquaDEB are i) to study and compare the sensitivity of aquatic species (mainly molluscs and fish) to environmental variability of natural or human origin, and ii) to evaluate the related consequences at different biological levels (individual, population, ecosystem) and temporal scales (life cycle, population dynamics, evolution). At mid-term life, the AquaDEB collaboration has already yielded interesting results by quantifying bio-energetic processes of various aquatic species (e.g. molluscs, fish, crustaceans, algae) with a single mathematical framework. It has also allowed to federate scientists with different backgrounds, e.g. mathematics, microbiology, ecology, chemistry, and working in different fields, e.g. aquaculture, fisheries, ecology, agronomy, ecotoxicology, climate change. For the two coming years, the focus of the AquaDEB collaboration will be in priority: (i) to compare energetic and physiological strategies among species through the DEB parameter values and to identify the factors responsible for any differences in bioenergetics and physiology; and to compare dynamic (DEB) versus static (SEB) energy models to study the physiological performance of aquatic species; (ii) to consider different scenarios of environmental disruption (excess of nutrients, diffuse or massive pollution, exploitation by man, climate change) to forecast effects on growth, reproduction and survival of key species; (iii) to scale up the models for a few species from the individual level up to the level of evolutionary processes.  相似文献   
25.
Sulfur and oxygen dynamics in the seagrasses Thalassia testudinum and Syringodium filiforme and their sediments were studied in the US Virgin Islands (USVI) in order to explore sulfide intrusion into tropical seagrasses. Four study sites were selected based on the iron concentration in sediments and on proximity to anthropogenic nutrient sources. Meadow characteristics (shoot density, above- and below-ground biomass, nutrient content) were sampled along with sediment biogeochemistry. Sulfide intrusion was high in T. testudinum, as up to 96% of total sulfur in the plant was derived from sediment-derived sulfides. The sulfide intrusion was negatively correlated to the turnover of sulfides in the sediments regulated by both plant parameters and sediment sulfur pools. Sediment iron content played an indirect role by affecting sulfide turnover rates. Leaf production was negatively correlated with sulfide intrusion suggesting that active growth reduced sulfide intrusion. Sulfide intrusion was lower in S. filiforme (up to 44%) compared to T. testudinum consistent with a higher internal nighttime oxygen concentrations found for S. filiforme. When S. filiforme can take advantage of its ability to maintain high internal oxygen concentrations, as was the case on the USVI, it could increase its success in colonizing unvegetated disturbed sediments with potentially high sulfide concentrations.  相似文献   
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The Betics are a key area to study an orogenic landscape disrupted by late‐orogenic extension. New low‐temperature thermochronology (LTT) data (AHe and AFT) coupled with geomorphic constraints in the Sierra de Gador (Alpujarride complex) are used to reconstruct the cooling history and evolution of relief during the Neogene. We document three stages: (1) a fast cooling event between 23 and 16 Ma associated with the well‐known extensive tectonic exhumation of the Alpujarride unit, (2) a period of slow cooling between 16 and 7.2 Ma related to a planation event and (3) a post‐7.2 Ma surface uplift associated with the inversion of the Alboran domain undetected by LTT. The planation event followed by this late uplift can explain the occurrence of inherited low‐relief surfaces overlain by Tortonian–Messinian platform deposits at the top of the range. Finally, we propose that the Sierra de Gador is a more transient landscape than the nearby Sierra Nevada.  相似文献   
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Establishing the age and crustal nature of exotic terranes and their underlying basements helps to determine their paleogeographic origin and tectonic histories. We present U–Pb ages of zircons and Sm–Nd whole rock isotopic data for volcanic and plutonic rocks of the Carolina terrane, one of several peri-Gondwanan terranes that were accreted to the margins of the circum-Atlantic continents during the Paleozoic. Volcanism in this subduction-related arc culminated in the eruption of the Morrow Mountain rhyolite, at ca. 540 Ma; thus, magmatism in the Carolina terrane ceased at the beginning of the Cambrian. The presence of inherited zircons and non-juvenile depleted mantle model ages of Carolina slate belt rocks favor a basement that is, at least in part, composed of evolved continental crust. Ages of inherited xenocrystic zircons cluster at ca. 1000, 2100 and 2500 Ma. These ages, in addition to volcanism at ca. 618–540 Ma, correlate best with well-known tectonic events in present-day northern South America. Specifically, the Orinoquian-Sunsas, the Trans-Amazonian and the Central Amazonian orogenic zones are likely candidates for potential basement correlatives to the Carolina terrane. Sm–Nd isotopic signatures vary significantly, but permit assimilation of Orinoquian age (1000 Ma) crust by magmas derived from the depleted mantle in a subduction (arc-related) setting. Our findings are also consistent with proposed correlations between the Carolina terrane and Avalonia which is likewise believed to have formed along the northern margin of present-day South America.  相似文献   
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