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31.
Acetylcholinesterase in mussel is potentially a useful biomarker of exposure to organophosphates (OP) in the marine environment. This study looked at cholinesterase activity in subcellular fractions of various tissues from the common mussel, Mytilus edulis. Measurement of enzyme rates demonstrated that although highest specific activity was found in foot 'mitochondrial' fraction, recovery of activity was very low. Gill 'microsomal' fraction had the second highest specific activity with a useful level of recovery and therefore was the most suitable tissue fraction for biomarker applications. Comparative studies of alternative alkylthiocholine substrates and competitive inhibitors suggest there is a single cholinesterase enzyme type present in this fraction. Inhibition of alkylcholine hydrolysis by BW284C51, specific to acetylcholinesterase in vertebrates, showed that cholinesterase activity in gill 'microsomal' fraction is inhibited by this compound but to a lesser extent than in vertebrate AChE. Inhibition of cholinesterase activity by azamethiphos in gill 'microsomal' fraction gave an IC50 of approximately 100 microM and showed both time and concentration dependence. However this indicates a lower potency compared to other animals and it is debatable whether mussel cholinesterase activity is useful as a biomarker of exposure in the field. 相似文献
32.
Gowlan BT McIntosh AD Davies IM Moffat CF Webster L 《Marine environmental research》2002,54(3-5):231-235
An aluminium smelter on the west coast of Scotland discharges an aqueous effluent containing polycyclic aromatic hydrocarbons (PAHs) at the head of Loch Leven. The loch also supports two mussel (Mytilus edulis) farms. Data are presented on burdens of PAHs in the soft tissues of mussels and the effect of these contaminants on glutathione S-transferase (GST) activity in mussel hepatopancreas. GST activity is shown to be correlated with total PAH burden and also with the concentrations of certain individual PAHs. These field data show that high molecular weight PAHs are closely correlated to GST activity, whereas low molecular weight PAHs are not. This suggests that 5- and 6-ring PAHs have a more pronounced role than 2- to 4-ring compounds in inducing GST activity in mussels from Loch Leven. It is proposed that it may be more appropriate to link GST activity with 5- and 6-ring compounds only, rather than with the total PAH burden. 相似文献
33.
The relatively wide KwaZulu-Natal Bight between St Lucia and Durban on the north-east shelf of South Africa is characterised by several circulation features driven by the Agulhas Current, wind and coastal inputs. A large multidisciplinary programme investigated the sources and relative influences of nutrients on the shelf. Within this, and to address a critical knowledge gap, this study describes macrobenthic (<1 mm) composition and frequency from 16 stations, assigned amongst four oceanographic focus areas. The areas were predetermined across the disciplines to represent upwelling, outwelling and a semi-persistent eddy, with nutrients and primary productivity being measured at each. Environmental variables such as sediment distribution, sediment TOC and bottom water physico-chemistry were determined at a significantly larger spatial scale. Our study postulated that oceanographic focus areas support significantly different macrobenthic assemblages, and that composition and relative distribution is due to measurable habitat attributes at each. Macrofauna were relatively abundant and particularly rich at >1 000 taxa. Annelida, Arthropoda, Mollusca, Echinodermata, Sipuncula and Cnidaria (>50 taxa each) were the dominant macrobenthic groups in the bight. Annelida were dominated by the polychaete families Spionidae, Terrebelidae and Cirratullidae, which were generally associated with outwelling and a mud depocentre off the Thukela River. Two unique and distinctive assemblages were found, one in the Thukela Mouth focus area and another on the midshelf between Thukela and Durban. The latter is influenced by poorly sorted, coarse sand and with probable influences from the Durban Eddy. There assemblages were abundant, rich and specific to this habitat. Correlation, PERMANOVA and CAP analyses showed assemblage fidelity to the focus areas. Medium sand, fine sand, mud and the variance of overall sediment type were the habitat drivers underlying macrofaunal abundance distributions. 相似文献
34.
G. M. Hill A. F. J. Moffat † N. St-Louis P. Bartzakos 《Monthly notices of the Royal Astronomical Society》2000,318(2):402-410
We have obtained complete phase coverage of the WC7+O binaries WR 42 = HD 97152 and WR 79 = HD 152270 with high signal-to-noise ratio (S/N), moderate-resolution spectra. Remarkable orbital phase-locked profile variations of the C iii λ 5696 line are observed and interpreted as arising from colliding wind effects. Within this scenario, we have modelled the spectra using a purely geometrical model that assumes a cone-shaped wind–wind interaction region which partially wraps around the O star. Such modelling holds the exciting promise of revealing a number of interesting parameters for WR+O binaries, such as the orbital inclination, the streaming velocity of material in the interaction region and the ratio of wind momentum flux. Knowledge of these parameters in turn leads to the possibility of a better understanding of WR star masses, mass-loss rates and wind region characteristics. 相似文献
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J.W. Moffat 《Astrophysics and Space Science》2003,283(4):505-509
Two variable speed of light models and their physical consequences are investigated.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
38.
A detailed analysis of the 2006 November 15 data release X-ray surface density Σ-map and the strong and weak gravitational lensing convergence κ-map for the Bullet Cluster 1E0657-558 is performed and the results are compared with the predictions of a modified gravity (MOG) and dark matter. Our surface density Σ-model is computed using a King β-model density, and a mass profile of the main cluster and an isothermal temperature profile are determined by the MOG. We find that the main cluster thermal profile is nearly isothermal. The MOG prediction of the isothermal temperature of the main cluster is T = 15.5 ± 3.9 keV , in good agreement with the experimental value T = 14.8+2.0 −1.7 keV . Excellent fits to the 2D convergence κ-map data are obtained without non-baryonic dark matter, accounting for the 8σ spatial offset between the Σ-map and the κ-map reported in Clowe et al. The MOG prediction for the κ-map results in two baryonic components distributed across the Bullet Cluster 1E0657-558 with averaged mass fraction of 83 per cent intracluster medium (ICM) gas and 17 per cent galaxies. Conversely, the Newtonian dark matter κ-model has on average 76 per cent dark matter (neglecting the indeterminant contribution due to the galaxies) and 24 per cent ICM gas for a baryon to dark matter mass fraction of 0.32, a statistically significant result when compared to the predicted Λ-cold dark matter cosmological baryon mass fraction of 0.176+0.019 −0.012 . 相似文献
39.
P. Bartzakos A. F. J. Moffat †‡ V. S. Niemela ‡§ 《Monthly notices of the Royal Astronomical Society》2001,324(1):18-32
A nearly complete sample of 24 Magellanic Cloud WC/WO subclass Wolf–Rayet stars is studied spectroscopically and photometrically to determine its binary frequency. Theory predicts the Roche lobe overflow produced Wolf–Rayet binary frequency to be 52±14 per cent in the Large Magellanic Cloud and 100 per cent in the Small Magellanic Cloud, not counting non-Roche lobe overflow Wolf–Rayet binaries. Lower ambient metallicity ( Z ) leads to lower opacity, preventing all but the most massive (hence luminous) single stars from reaching the Wolf–Rayet stage. However, theory predicts that Roche lobe overflow even in binaries of modest mass will lead to Wolf–Rayet stars in binaries with periods below approximately 200 d, for initial periods below approximately 1000 d, independent of Z . By examining their absolute continuum magnitudes, radial velocity variations, emission-line equivalent widths and full widths at half-maximum, a WC/WO binary frequency of only 13 per cent, significantly lower than the prediction, is found in the Large Magellanic Cloud. In the unlikely event that all of the cases with a less certain binary status actually turn out to be binary, current theory and observation would agree. (The Small Magellanic Cloud contains only one WC/WO star, which happens to be a binary.) The three WC+O binaries in the Large Magellanic Cloud all have periods well below 1000 d. The large majority of WC/WO stars in such environments apparently can form without the aid of a binary companion. Current evolutionary scenarios appear to have difficulty explaining either the relatively large number of Wolf–Rayet stars in the Magellanic Clouds, or the formation of Wolf–Rayet stars in general. 相似文献
40.
G. Stowasser R. McAllen G.J. Pierce M.A. Collins C.F. Moffat I.G. Priede D.W. Pond 《Deep Sea Research Part I: Oceanographic Research Papers》2009,56(5):812-826
To investigate the trophic ecology of two of the dominant families of deep-sea fish (Macrouridae and Moridae) fatty acid and stable isotope analyses were applied to liver and muscle samples of five abundant species from the NE Atlantic. In conjunction with stomach content data these methods made it possible to identify differences in feeding strategies between the five study species as well as variation in feeding in relation to increasing depth and body size. Biomarkers identified strong similarities between Coryphaenoides armatus and Antimora rostrata though differences were found associating C. armatus more with the benthic food web whereas A. rostrata showed stronger links to the pelagic food web. While Lepidion eques was classified as a species linking benthic and benthopelagic food webs, both fatty acid and stable isotope data suggested that Coryphaenoides guentheri fed on an exclusively benthic diet. Coryphaenoides rupestris on the other hand were largely dependent on a copepod-based food web. Ontogenetic changes in feeding were found for both A. rostrata and C. armatus with the indication of a switch from active predation to scavenging occurring with increasing body size. Biomarkers also reflected the seasonal influx from the photic zone though changes were species-specific and probably reflected the variation in prey availability and abundance in response to these inputs. Our findings have thus demonstrated that the combined use of these biomarkers can elucidate trophic specialisations in situations where conventional methods alone previously provided insufficient data. 相似文献