Wind-driven interannual variability over the northeast Pacific Ocean     

Patrick F. Cummins  Gary S.E. Lagerloef 《Deep Sea Research Part I: Oceanographic Research Papers》2004,51(12):365

Interannual variability of the sea surface height (SSH) over the northeast Pacific Ocean is hindcast with a reduced-gravity, quasi-geostrophic model that includes linear damping. The model is forced with monthly Ekman pumping fields derived from the NCEP reanalysis wind stresses. The numerical solution is compared with SSH observations derived from satellite altimeter data and gridded at a lateral resolution of 1 degree. Provided that the reduced gravity parameter is chosen appropriately, the results demonstrate that the model has significant hindcast skill over interior regions of the basin, away from continental boundaries. A damping time scale of 2 to 3 years is close to optimal, although the hindcast skill is not strongly dependent on this parameter.A simplification of the quasi-geostrophic model is considered in which Rossby waves are eliminated, yielding a Markov model driven by local Ekman pumping. The results approximately reproduce the hindcast skill of the more complete quasi-geostrophic model and indicate that the interannual SSH variability is dominated by the local response to wind forcing. There is a close correspondence the two leading empirical orthogonal modes of the local model and those of the observed SSH anomalies. The latter account for over half of the variance of the interannual signal over the region.  相似文献   

Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)     

Blum JL  Knoebl I  Larkin P  Kroll KJ  Denslow ND 《Marine environmental research》2004,58(2-5):565-569

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.  相似文献   

Contaminant concentrations and toxicity of sea-surface microlayer near Los Angeles, California     

Jeffrey N. Cross  John T. Hardy  Jo Ellen Hose  G.Patrick Hershelman  Liam D. Antrim  Richard W. Gossett  Eric A. Crecelius 《Marine environmental research》1987,23(4)

Sea-surface microlayer samples were collected from six nearshore areas receiving different amounts of anthropogenic inputs. The samples were analyzed for selected trace metals, chlorinated hydrocarbons, and polycyclic aromatic hydrocarbons. The relative toxicities of the samples were determined with fish embryo bioassays.Contaminant concentrations generally increased from offshore to the inshore stations. Contaminant concentrations were several orders of magnitude higher in microlayer samples from the highly industrialized Los Angeles and Long Beach harbors compared to samples from a site 15km offshore. Microlayer samples from the inshore stations were significantly more toxic, and induced significantly more developmental abnormalities and chromosome aberrations, than samples from the offshore stations.  相似文献   

Swath mapping of the seafloor and its application to deep-bottom fisheries in New Caledonia     

Patrick Lehodey  René Grandperrin 《Marine Geophysical Researches》1996,18(2-4):449-458

A bottom longline fishery operated in the EEZ of New Caledonia from 1988 to 1991. Fishing focused on five seamounts with summits at depths ranging from 500 to 750 m. The target species was alfonsino, Beryx splendens. As the soundings available from marine charts were not detailed enough, the fishing masters had to make their own charts in order to set the gear in the right location. A series of 11 scientific cruises devoted to a research program on alfonsino was launched in late 1991. During the first scientific cruises, several days were spent mapping the seabed to improve the existing knowledge of the topography of three seamounts by coupling the EDO echosounder depth measurements to the GPS positions. As this procedure is slow, it is applicable to limited areas or, if a wider grid is used, to large zones to locate major structures such as ridges and seamounts. The emergence of the multibeam echosounder has greatly improved seabed mapping performance. This tool seems to fit the requirement of exploratory deep-bottom fishing particularly well, as it covers large areas while providing details of the bottom. The EM 12 was used to obtain bathymetry and imagery of the SE portion of the EEZ of New Caledonia, covering an approximate area of 70,000 km² in two weeks. For depths ranging from 500 to 4,500 m, the results were impressive. They confirmed the known major features and provided greater topographical detail, revealing fine unknown structures. They also provided information on the type of substratum, information which might influence the fishing strategy. Finally, they made it possible to obtain an accurate estimate of the exploitable area which in turn led to new stock estimates.  相似文献   

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Respiratory Rate and Assessment of Secondary Production in the Holothuroid Holothuria tubulosa (Echinodermata) from Mediterranean Seagrass Beds     

Pierre  Coulon  Michel  Jangoux Patrick  Bulteel 《Marine Ecology》1992,13(1):63-68

Abstract. Respiration in Holothuria tubulosa was investigated in individuals from the Posidonia oceanica meadow off Lacco Ameno (Ischia Island, Italy). Respiratory rates increase with increasing body weight and increasing sea water temperature. Oxygen consumption of an average individual (7g dw body wall) ranges from 0.409 (14 °C) to 1.300 (26 °C) mg O₂· h^-1. Data on population density, mean size of individuals, and annual sea water temperature variations allow an assessment of holothuroid production. Values of 45.65 and 13.75 kJ · m^-2· y^-1 were calculated for shallow (3 to 10 m) and deep (25 to 33 m) areas of the Posidonia meadow, respectively. Holothuroid production shows a bathymetric pattern similar to primary production of the Posidonia -epiphytes complex and the production of Posidonia litter.  相似文献   

Nonparametric Estimates of the Sea State Bias for the Jason-1 Radar Altimeter   总被引：3，自引：0，他引：3  

Sylvie Labroue  Philippe Gaspar  Joel Dorandeu  Oz Zanif    F. Mertz  Patrick Vincent  Denis Choquet 《Marine Geodesy》2004,27(3):453-481

The Jason-1 sea state bias (SSB) is analyzed in depth from the first year of GDR products. Compared to previous missions, this work benefits from two aspects of the empirical determination of the SSB from the altimetric data themselves. First, from a methodological point of view, a nonparametric technique (NP) has been developed and largely tested on TOPEX/Poseidon 1, GFO and Envisat data. The NP estimator has proven to be a useful tool in the SSB estimation, and it is now mature enough to be used for a refined analysis. On the other hand, the SSB can be extracted from three different data sets (crossovers, collinear data, and residuals) with different characteristics. It is then possible to cross calibrate various estimations of the SSB models and to determine the most accurate one. A systematic comparison is made between these different estimates for the Jason-1 altimeter. The collinear and crossover data sets yield very similar estimates despite their difference of spatial and temporal distributions. These SSB models assure consistency with the TOPEX mission when comparing Jason-1 and TOPEX residuals during the tandem phase. Thanks to the present work, the impact of the short wavelengths filtering on the SSB estimation is evidenced. More generally, our understanding of potential errors affecting the sea surface height and their impact onto the SSB estimation is also improved.  相似文献   

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels   总被引：5，自引：0，他引：5  

Scholz S  Kordes C  Hamann J  Gutzeit HO 《Marine environmental research》2004,57(3):235-244

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.  相似文献   

Investigation of the stable isotope fractionation in speleothems with laboratory experiments     

E. Wiedner  D. Scholz  A. Mangini  D. Polag  C. Mühlinghaus  M. Segl 《Quaternary International》2008,187(1):15

Many speleothems show evidence for calcite precipitation under disequilibrium conditions. To improve the understanding of these kinetic processes, several laboratory experiments were performed to study the fractionation of stable oxygen and carbon isotopes during the precipitation of calcite. Carbonate was precipitated under controlled conditions from both a body of standing water (beaker experiments) and a solution flowing along a channel (channel experiments) at a relative humidity of 100%. Slow degassing of CO₂, simulated by the beaker experiments, results in δ¹⁸O values in equilibrium with the solution. In contrast, the δ¹³C values show a significant enrichment, inversely proportional to the height of the solution in the beakers. Fast degassing of CO₂, simulated by the channel experiments, showed an enrichment of both δ¹³C and δ¹⁸O and a slope of Δδ¹³C/Δδ¹⁸O of 1.4±0.6. These results represent experimental evidence for the Hendy effect, which is manifested in (i) a progressive increase in δ¹⁸O and δ¹³C away from the growth axis and (ii) a positive correlation between δ¹⁸O and δ¹³C along a single growth layer of a stalagmite.  相似文献   

New age constraints on emplacement of the Cévenol granitoids, South French Massif Central     

Stéphanie Brichau  Jean-Patrick Respaut  Patrick Monié 《International Journal of Earth Sciences》2008,97(4):725-738

During the development of the Variscan orogeny, large amounts of granitic melt were produced, giving rise to the intrusion of granitoids at different structural levels. Despite numerous studies, ages available from previous work on the Cévennes granites remain largely imprecise. In order to better constrain the age and emplacement mode of these granites, we have combined U–Pb dating on monazites and zircons and ⁴⁰Ar/³⁹Ar dating on biotites with petrological observations, major element chemical analysis and SEM zircon imaging on five samples from the Aigoual–St Guiral–Liron and Mont Lozère granitic massifs. The results revealed that granitic intrusions and cooling in Southern Cévennes occurred in a short time span at ∼306 Ma after the main episode of regional metamorphism. Petrological and chemical data suggest that they result from a mixing between mantle-derived basic magmas (lamprophyres) and lower crust acid magmas. At a regional scale the production of these melts occurred at the end of crustal thickening induced by nappe stacking, at the same time as the late anatectic events recorded further north in the Velay dome and the granulite facies metamorphism recorded in metasedimentary granulite enclaves brought up by Tertiary volcanoes of the Velay area (Bournac).  相似文献