Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

游客-环境互动视角下旅游者目的地环境恢复性效应     

朱芳  苏勤  陶云  沈惊宏 《热带地理》2020,40(4):636-648

日常生活环境和旅游目的地环境都可以为个体提供恢复的机会，不同环境的属性特征导致不同程度的恢复。为了探究环境类型与恢复性效应之间的关系及停留时间的调节作用，文章采用问卷调查方式，利用配对样本t检验和混合设计方差分析相结合的方法，探讨游客在不同环境中的恢复性效应，并对这一现象提供心理上的解释。结果表明：1）恢复体验的4个维度（放松体验、心理脱离、掌握体验、控制体验）在旅游目的地环境中的得分均高于日常生活环境。2）掌握和控制体验维度在城市旅游目的地中的变化程度高于乡村旅游目的地，两种类型目的地的放松和心理脱离维度差异较小。3）随着在目的地停留时间的延长，乡村旅游目的地放松体验和心理脱离维度变化程度高于城市旅游目的地，两种类型目的地环境恢复性效应会相互靠近。因此，游客的环境恢复性效应不仅受到环境和目的地类型的影响，同时还与游客的适应程度有关，且受到其停留时间的调节作用。  相似文献   

观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

  相似文献   

An approximate method for evaluating the shear band thickness in saturated soils     

X. B. Lu  Wang Shuyun  Wang Yihua  Peng Cui 《国际地质力学数值与分析法杂志》2004,28(15):1533-1541

A formula for the thickness of a shear band formed in saturated soils under a simple shear or a combined stress state has been proposed. It is shown that the shear band thickness is dependent on the pore pressure properties of the material and the dilatancy rate, but is independent of the details of the combined stress state. This is in accordance with some separate experimental observations. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

GPS/LEO掩星观测的变分同化技术   总被引：6，自引：0，他引：6  

刘敏  郭鹏 《天文学进展》2006,24(1):27-42

在简单介绍GPS/LEO掩星探测大气的发展历史和科学意义之后,详细阐述了反演的基本原理;分析了标准反演中存在的问题,并说明一维变分同化(1DVAR)在反演方法中的重要性;给出了一维变分同化中价值函数的求解,以及各种同化因子;简单介绍了对当前气象学中普遍使用的四维变分同化(4DVAR);重点讨论了各种同化方法,以及使用各种同化因子的优缺点。最后,通过CHAMP卫星的观测实例分析,验证了GPS数据在数值天气预报(NWP)中的作用,以及相对于标准反演法一维变分对气象要素的改进。  相似文献   

Approach to Mountain Hazards in Tibet, China   总被引：1，自引：1，他引：0  

MADongtao TUJianjun CUIPeng LURuren 《山地科学学报》2004,1(2):143-154

Tibet is located at the southwest boundary of China. It is the main body of the Qinghai-Tibet Plateau, the highest and the youngest plateau in the world. Owing to complicated geology, Neo-tectonic movements, geomorphology, climate and plateau environment, various mountain hazards, such as debris flow, flash flood, landslide, collapse, snow avalanche and snow drifts, are widely distributed along the Jinsha River (the upper reaches of the Yangtze River), the Nu River and the Lancang River in the east, and the Yarlungzangbo River, the Pumqu River and the Poiqu River in the south and southeast of Tibet. The distribution area of mountain hazards in Tibet is about 589,000 km^2, 49.3% of its total territory. In comparison to other mountain regions in China, mountain hazards in Tibet break out unexpectedly with tremendously large scale and endanger the traffic lines, cities and towns, farmland, grassland, mountain environment, and make more dangers to the neighboring countries, such as Nepal, India, Myanmar and Bhutan. To mitigate mountain hazards, some suggestions are proposed in this paper, such as strengthening scientific research, enhancing joint studies, hazards mitigation planning, hazards warning and forecasting, controlling the most disastrous hazards and forbidding unreasonable human exploring activities in mountain areas.  相似文献   

Microwave Type III Burst Group in the 1993 October 2 Flare     

Qijun Fu  Zhihai Qin  Yuying Liu  Guangli Huang 《Astrophysics and Space Science》1997,250(2):189-204

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.  相似文献   

Optical monitoring of the quasar 4C 38.41     

B. Peng  J. Wu  X. Zhou 《Monthly notices of the Royal Astronomical Society》2003,346(2):483-488

The results of a photometric monitoring of the quasar 4C 38.41, performed at the optical R and B bands in 2002 February–March, are presented. With a 60/90 cm Schmidt telescope at the Xinglong station of the National Astronomical Observatories of China, we observed the source exhibiting amplitude variations of up to 0.78 mag in both bands during the whole campaign. Intraday and even intranight variations are detected as well. A typical variability time-scale of about 36 d is derived from our 2-month observations at the optical bands, which is identical to that found at a radio wavelength of 92 cm, suggesting a common origin for the variations in 4C 38.41 from optical to radio bands.  相似文献   

SIMULATION AND PREDICTION OF DEBRIS FLOW USING ARTIFICIAL NEURAL NETWORK     

WANGXie-kang HUANGEr CUIPeng 《中国地理科学(英文版)》2003,13(3):262-266

Debris flow is one of the most destructive phenomena of natural hazards. Recently, major natural haz-ard, claiming human lives and assets, is due to debris flow in the world. Several practical methods for forecasting de-bris flow have been proposed, however, the accuracy of these methods is not high enough for practical use because of the stochastic and non-linear characteristics of debris flow. Artificial neural network has proven to be feasible and use-fill in developing models for nonlinear systems. On the other hand, predicting the future behavior based on a time se-ries of collected historical data is also an important tool in many scientific applications. In this study we present a three-layer feed-forward neural network model to forecast surge of debris flow according to the time series data collect-ed in the Jiangjia Ravine, situated in north part of Yunnan Province of China. The simulation and prediction of debris flow using the proposed approach shows this model is feasible, however, further studies are needed.  相似文献