我国的鱼类细胞核移植??     

李荔??  张士璀??  王洪海??  张福玲 《海洋科学》2001,25(3):25-27

鱼类作为水产养殖业的一大支柱品种 ,其良种培育对提高鱼类产量至关重要。我国是鱼类养殖历史最悠久的国家之一 ,向来重视应用生物技术对养殖品种进行改良 ,其中最好的例子之一就是利用细胞核移植技术来创造鱼类优良品种。即使在生物技术不断发展的今天 ,这一细胞核移植技术在鱼类新品种培育方面仍是一项很有潜在应用价值的技术。特别是把细胞核移植技术与现代生物技术相结合 ,有望创造出具有巨大经济价值的鱼类新品种。１我国鱼类细胞核移植的成果细胞核移植是一种应用精细的显微操作 ,将一种动物的细胞核移植到同种或异种动物的去核成熟卵…  相似文献   

湄洲湾表层沉积物中重金属的地球化学特征   总被引：6，自引：2，他引：6  

许爱玉  陈松 《台湾海峡》1993,12(1):16-20

根据1984年湄洲湾表层沉积物中重金属的分析资料,讨论了它们的地球化学特征和主要来源。沉积物中重金属的含量(Fe、Mn、Cu、Ni 的质量分数均值分别为3.21%、730×10~(-6)、19.4×10~(-6)、44×10~(-6)分布比较均匀,Pb、Zn 和Co 含量(质量分数均值分别为47.0×10~(-6)、25.8×10~(-6)和243×10~(-6))偏高,微量金属含量不受Fe-Mn 氧化物和有机物控制,本地区的金属来源受陆源输入的影响明显。  相似文献   

1986—1987年厄尔尼诺-南方涛动事件形成发展过程分析   总被引：1，自引：0，他引：1  

武建平  李若钝 《热带海洋学报》1988,(4)

本文利用近期热带太平洋大气—海洋实测资料,对起始于1986年9月的厄尔尼诺(EI-Ni(?)o)-南方涛动(ENSO)事件作了初步分析,发观亦道太平洋月际STS距平、纬向风指数、平均海平面高度距平、南方涛动指数、地球射出长波辐射、大气环流遥相关型和西太平洋副热带高压等在这次ENSO事件发生前后均有显著变化。作者指出,这次ENSO事件中,赤道太平洋海温异常增暖不是象通常那样发生在春季和由南美沿岸向西发展,而是发生在秋季,且首先出现在中赤道太平洋日界线区,而后迅速向东发展,从海温异常增暖的强度和持续时间来看,这次ENSO事件基本属一次中等强度过程。  相似文献   

DYNAMIC ANALYSIS OF TYPHOON SURGES ALONG THE COASTS OF ZHEJIANG AND JIANGSU PROVINCES   总被引：1，自引：0，他引：1  

陈长胜  秦曾灏 《海洋学报(英文版)》1985,4(4):515-526

Based on the numerical model shown in Ref. [1], a dynamic analysis is given for the storm surges caused by the four typhoons (i. e. typhoons Nos. 5310, 5612, 6126 and 6207) on the east coast of Zhejiang and Jiangsu Provinces, in which the contributions of various dynamic factors to local surge elevation are calculated numerically, including the Coriolis force, the atmospheric pressure force, the bottom friction, the local topography and the couple nonlinear interactions between surge current and elevation as well as between surge currents themselves. Moreover, the responses of these factors to different kinds of typhoon tracks and water depths are discussed in more detail, and the effects of grid size on the computed results demonstrated.  相似文献   

利用ARC／lNFO建立S-57数据模型的研究   总被引：2，自引：2，他引：2  

纪宏宇黄忠刚  陈高兴顾锦华 汪海 《海洋测绘》2002,22(1):11-17

数字海图的标准化、规模化生产，是发展电子海图显示与信息系统(ECDIS)、在世界范围内提供安全可靠的商业化数字海图产品的重要前提。S-57是国际海道测量组织(IHO)颁布的国际通用的数字海道测量数据传输标准，该标准已为越来越多的国家所采用，用来进行数字海图数据的传输。本文介绍了海图数据库建设中对S-57的一些研究情况，提出了如何利用ARC/INFO)平台建立S-57数据体系的参考模式，并对未来电子航海图(ENC)生产和质量检测流程的建立模式加以探讨。  相似文献   

Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans     

Minutoli R  Fossi MC  Guglielmo L 《Marine environmental research》2002,54(3-5):799-804

Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.  相似文献   

南黄海盆地油气地质研究进展     

陈建文  吴志强 《海洋地质前沿》2002,18(1):22-22

青岛海洋地质研究所近年来广泛收集了国内外在南黄海海域的地质与地球物理资料 ,对南黄海盆地的石油地质特征进行了系统的研究 ,并应用海洋“863”高科技成果软件——《海上油气资源综合快速评价系统》进行了盆地模拟和资源评价 ,取得了多项创新性的进展。( 1 )广泛应用国内外在南黄海海域及邻区的重磁资料和地震资料 (包括新采集的多道地震资料 ) ,采用综合地球物理解释技术 ,首次编制了全盆地 1∶ 50万的基底构造图、深度构造图、厚度图及沉积相图 ,对盆地的构造区划进行了重新划分 ,为南黄海油气勘查部署打下了良好基础。( 2 )在南黄海北…  相似文献   

An aromatase inhibitor and tamoxifen decrease plasma levels of o,p'-DDT and its metabolites in Nile tilapia (Oreochromis niloticus)     

Leaños-Castañeda O  Gold-Bouchot G  Van Der Kraak G  Lister A  Ceja-Moreno V  Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342

The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT.  相似文献   

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

生产率的测定     

EdBoyle陈秀澄  周立君 《海洋地质前沿》1994,(3):15-17

冰芯记录表明，在过去的冰期期间大气二氧化碳的浓度减小了．为什么会这样?在极地大洋生长的浮游生物是主要怀疑对象，因为CO2通过光合作用被吸收，而在有机物沉到洋底时停止了循环．对于气候来说，非常需要对浮游生物生产率进行一次很好的测定．  相似文献