Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR     

Yang N  Chu DL  Wong MM  Qi H  Wu RS  Kong RY 《Marine pollution bulletin》2011,62(12):2654-2658

Marine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5′-noncoding region (5′-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichiacoli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters.  相似文献   

A whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish     

Han XB  Lei EN  Lam MH  Wu RS 《Marine pollution bulletin》2011,63(5-12):160-165

Polybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5 ng/L, 1 μg/L and 50 μg/L of DE-71 for the duration of the whole life cycle (120 days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined. In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50 μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated. Serum estradiol (E2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E2 as well as the ratio of 11-KT/E2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones.  相似文献   

汶川地震前后地震波速比和视速度变化特征的研究   总被引：5，自引：0，他引：5       下载免费PDF全文

王林瑛  李艳娥  李芳  李广平  张天中  包淑娴  苏金蓉 《地震学报》2011,33(1):1-14

2008年5月12日在中国四川省境内龙门山断裂带发生了8级巨大地震．在其主震前后随着震源区附近应力状态的改变,地壳介质的物性是否也存在相应的异常变化过程？该问题成为汶川地震研究的热点科学问题之一．本研究搜集和整理了中国地震局四川数字地震台网2001年1月1日-2010年5月31日产出的震相观测报告,和中国地震局地球物理研究所流动数字地震台网2008年5月12日——9月30日产出的震相观测报告,采用多台和达法和多地震联合测定法,根据入选的直达波Pg和Sg的震相数据,重点研究了龙门山断裂及其附近地区的P波和S波视速度的时间变化特征,并与波速比的时间变化特征进行了对比分析．为保证计算结果的可靠性和稳定性,对所收集到的数据进行了较为严格的筛选和限定．研究发现,龙门山断裂及其附近地区,在汶川地震前P波和S波视速度存在4年左右的明显降低过程,到震前约半年出现快速恢复．在震前P波和S波的视速度明显降低的过程中,波速比的低值异常过程仅为2年多,且幅度小持续时间较短．在波速比和视速度异常发展的进程中,进入发震中短期异常快速恢复是两者的共同特征．   相似文献   

Spatio-temporal changes of marine macrobenthic community in sub-tropical waters upon recovery from eutrophication. I. Sediment quality and community structure   总被引：1，自引：0，他引：1  

Shin PK  Lam NW  Wu RS  Qian PY  Cheung SG 《Marine pollution bulletin》2008,56(2):282-296

A two-year study was conducted in the vicinity of a harbour in sub-tropical Hong Kong, to examine the progress of improvement in sediment quality and recovery of macrobenthic community upon cessation of organic pollution caused by sewage discharge. Median sediment particle diameter (Mdphi) and levels of total organic carbon (TOC), total Kjeldahl nitrogen (TKN), ammonia-nitrogen (NH(3)-N) and total phosphorus (TP), as well as macrobenthic species composition and abundance were determined bi-monthly at three inside-harbour and two outside-harbour locations. At the two inside-harbour locations, significantly higher levels of TOC, TKN, NH(3)-N and TP in sediments were observed than the outside-harbour locations. However, no significant temporal change of nutrient levels was detected at all sampling locations during the two-year study, except a significant decrease in TKN and NH(3)-N levels at one outside-harbour location and a decline in NH(3)-N content at another outside-harbour location. Spatially, the highest in mean total species number, individual number, species diversity H' and lowest evenness J was found at one outside-harbour location, whereas the other four locations had relatively similar values. H' was negatively correlated with TOC, TKN, NH(3)-N and TP content in sediments while J was positively correlated with MDphi. Across the study period, the patterns of macrobenthic community were significantly different among all samplings and that the spatial difference of benthic community was best correlated with MDphi, TOC, TKN and water depth. A weak sign of recovery in macrobenthic community structure was detected at inside-harbour locations, with replacement of opportunistic by ubiquitous species over the two-year study. The slow recovery of macrobenthic community was related to sediment characteristics. Results of a larval settlement bioassay using the polychaete Capitella sp. I also demonstrated that the inside-harbour sediments were still unfavourable for colonization and larval settlement of species sensitive to pollution. The slow biodegradation of the organic pollutants and continuous presence of heavy metals in the sediment may hinder settlement and colonization of benthic animals. However, increases of H' and J were observed in a longer time scale when comparing the present data with those obtained four years ago. This suggested that a detectable recovery of benthic community in the harbour may take at least three years and a complete recovery may even take longer duration.  相似文献   

Temporal vP/vS variation characteristics in different zones of China’s Capital Circle area before and after Wen’an earthquake     

王林瑛  郭永霞  刘芳  蒋长胜 《地震学报(英文版)》2008,21(3):243-257

On July 4, 2006, an earthquake of MS5.1 took place in Wen’an, Hebei Province, just at the south center of China’s Capital Circle area digital seismograph network. It is the strongest event recorded ever since the network went into operation in 2002. We processed the vast amounts of phase data yielded by the 107 digital seismic stations between 2002~2007 using Wadati method. In order to improve the precision and stability of shear and compressional wave velocities (vP/vS) calculation, we impose a number of restrictions on the computation environment and condition, e.g., the earthquakes are densely concentrated, selected stations are limited in range, the number of stations in- volved in the computation is larger than 5 and linear fitting features high precision and small error. Under these restrictions, the study shows that vP/vS in and around Wen’an and Tangshan underwent a normal-low-normal proc- ess one year before Wen’an earthquake, vP/vS became obviously low and the low ratio lasted for about one year, meanwhile, little variation of vP/vS was seen in Xingtai, northwest of Beijing, southwest of Beijing, Beijing-Tianjin and Beijing; after the quake, the vP/vS returned normal in Wen’an and Tangshan. Error and stability analysis of the calculated result for vP/vS shows it is convincible that anomaly appeared in and around Wen’an and Tangshan be- fore Wen’an earthquake.  相似文献   

美国海军生物污损研究的历程及目标     

Albe.  RS 李静 《南海研究与开发》2000,(1):70-73

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由前次地震引起的应力触发1994年加州北岭M=6.7地震     

Stein  RS 耿杰 《地震地质译丛》1996,18(1):47-50

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新的洋壳地震图像     

Detr.  RS 李士范 《海洋地质译丛》1994,(1):72-77

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展望未来：新的观测系统及其对中尺度研究和业务的影响     

Sera.  RS 苏振生 《气象科技》1992,(4):32-35

1.引言过去10年间,发表了许多有关未来观测系统的文章和令人鼓舞的新技术的描述,如Serafin,Lhermitte和Atlas(1981);Serafin,Debberdt和Cooper(1987);Serafin,Szejwach和Phillips(1986);Strauch(1988);以及其他人的文章.把过去10年取得的进步同许多论文中对未来技术方法重大发展的预  相似文献   

波罗的地盾地区的地震     

Sluu.  RS 钱竞阳 《地震地质译丛》1992,14(5):37-41

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