酶解条件对条斑紫菜单细胞成活率影响的比较研究     

杨震  唐学玺  戴继勋  沈颂东  宫相忠  贾鹏举 《中国海洋大学学报(自然科学版)》2001,31(3):407-410

该文分别选用不同浓度的海螺酶 ;葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠 5种渗透剂 ;维生素 C和甘露醇 2种抗氧化剂 ;对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明 :用浓度为 10 %的海螺酶酶解条斑紫菜 ,成活率最高 ;选用 2 mol/ L葡萄糖作渗透剂 ,效果最好 ,成活率达85.2 % ;加入抗氧化剂对细胞成活率没有影响  相似文献   

Underice Operation of SJT-5 ROV     

Tang Rongqing  Wang Daoyan Zhang Xixian Egnineer  Shanghai Jiao Tong University  Shanghai  Associate Professor  Shanghai Jiao University  Shanghai 《中国海洋工程》1993,(3)

- The authors have used a low-cost ROV of observation type for underice surveying of the Nen-Jiang Cross-river oil pipe line in the Da Qing oil field. The operation method, process and the safety measures for severe cold areas are presented. The fruitful tries and experience may be useful to ROV operators in severe cold areas.  相似文献   

烧伤病人应用CHIMEHERB人工皮的临床评价     

唐晓熹  许伟石 《中国海洋大学学报(自然科学版)》1989,(Z3)

本研究初步评价CHIMEHERB人工皮的治疗效果.74例,105个创面应用人工皮:供皮区47个创面,愈合时间平均为9±1.94d(天),油纱布对照为11.7±1.93d(P<0.01);14个浅Ⅱ°新鲜创面,70％在3～5d内逐渐干燥与创面紧密粘附,平均愈合时间为9.8±3.09d;11个存留上皮组织的削痂深Ⅱ°创面,平均5.7±1.7d干燥,平均愈合时间为10.55±3.09d,不需植自体皮;8个削痂刨面至脂肪组织或切痂创面4～9d更植自体皮,其存活率近乎100％,2个创面因残留坏死组织而失败;人工皮应用于16个自体皮间隙创面,除三个网状植皮和一个条状植皮创面失败外,所植自体皮都在人工皮下扩展融合,未观察到占位现象;对后期残余小创面有良好的效果.本研究结果提示人工皮对创面愈合具有有利影响.主要适用于供皮区、浅Ⅱ°新鲜创面、深Ⅱ°脱痂和削痂后存留上皮组织较多的创面、自体皮间隙创面和后期残余小创面,不适宜于深Ⅲ°新鲜创面.  相似文献   

Processes Causing the Temporal Changes in Si/N Ratios of Nutrient Consumptions and Export Flux during the Spring Diatom Bloom     

Naoki?YoshieEmail author  Yasuhiro?Yamanaka 《Journal of Oceanography》2005,61(6):1059-1073

Two processes are generally explained as causes of temporal changes in the stoichiometric silicon/nitrogen (Si/N) ratios of sinking particles and of nutrient consumption in the surface water during the spring diatom bloom: (1) physiological changes of diatom under the stress of photosynthesis of diatom and (2) differences of regeneration between silicon and nitrogen. We investigated which process plays an important role in these changes using a one-dimensional ecosystem model that explicitly represents diatom and the other non-silicious phytoplankton. The model was applied to station A7 (41°30′ N, 145°30′ E) in the western North Pacific, where diatom regularly blooms in spring. Model simulations show that the Si/N ratios of the flux exported by the sinking particles at 100 m depth and of nutrient consumptions in the upper 100 m surface water have their maxima at the end of the spring diatom bloom, the values and timings of which are significantly different from each other. Analyses of the model results show that the differences of regeneration between silicon and nitrogen mainly cause the temporal changes of the Si/N ratios. On the other hand, the physiological changes of diatoms under stress can hardly cause these temporal changes, because the effect of the change in the diatom's uptake ratio of silicon to nitrogen is cancelled by that in its sinking rate.  相似文献   

A Zonal Pathway for NADW in the South Atlantic     

Nelson?G.?HoggEmail author  Andreas?M.?Thurnherr 《Journal of Oceanography》2005,61(3):493-507

Several large deployments of neutrally buoyant floats took place within the Antarctic Intermediate (AAIW), North Atlantic Deep Water (NADW), and the Antarctic Bottom Water (AABW) of the South Atlantic in the 1990s and a number of hydrographic sections were occupied as well. Here we use the spatially and temporally averaged velocities measured by these floats, combined with the hydrographic section data and various estimates of regional current transports from moored current meter arrays, to determine the circulation of the three major subthermocline water masses in a zonal strip across the South Atlantic between the latitudes of 19°S and 30°S. We concentrate on this region because the historical literature suggests that it is where the Deep Western Boundary Current containing NADW bifurcates. In support of this notion, we find that a net of about 5 Sv. of the 15–20 Sv that crosses 19°S does continue zonally eastward at least as far as the Mid-Atlantic Ridge. Once across the ridge it takes a circuit to the north along the ridge flanks before returning to the south in the eastern half of the Angola Basin. The data suggest that the NADW then continues on into the Indian Ocean. This scheme is discussed in the context of distributions of dissolved oxygen, silicate and salinity. In spite of the many float-years of data that were collected in the region a surprising result is that their impact on the computed solutions is quite modest. Although the focus is on the NADW we also discuss the circulation for the AAIW and AABW layers.  相似文献   

The fate of dumped sediments monitored by a high-resolution multibeam echosounder system,Weser Estuary,German Bight     

Claudia?WienbergEmail author  Jens?Dannenberg  Dierk?Hebbeln 《Geo-Marine Letters》2004,24(1):22-31

With the development of high-resolution multibeam echosounder systems (MBES) for surveying shallow-water areas a new tool is available to monitor rapid changes in seabed morphology as, e.g., caused by the dumping of dredge spoil in coastal waters. In this study, four data sets of repeated bathymetric surveys with a MBES were processed and analyzed. The data were collected in a 1.94-km² dumping site in the outer Weser Estuary (German Bight). Between June and December 1998, 2.6 million m³ of dredged sediment were deposited there. The bathymetric maps generated in the course of this study reveal features such as subaqueous dunes, scour holes, and mounds of dumped dredge spoil. The mean water depth decreased by about 1 m during the dumping period. Furthermore, difference grids showing changes in sediment volume allowed a calculation of the sediment budget for the monitored area. After a time period of only 5 months, 0.5 million m³ of the originally dumped 2.6 million m³ of dredge spoil had already been removed from the dumping site.  相似文献   

玻璃钢渔船船体材料切片之弯曲试验研究     

李文涛  唐衍力  黄六一 《海洋湖沼通报》2001,(1):18-25

玻璃钢渔船有很多优异的性能，因而有逐渐取代木质渔船的趋势。为推进玻璃钢渔船的产业化进程，作者在查阅了大量国内外有关资料的基础上，进行了玻璃钢渔船体材料切片的弯曲试验，最终提出了我国玻璃钢渔船船体材料切片的弯曲试验规程，以供我国今后制定玻璃钢渔船材料试验标准作参考。  相似文献   

东海温度锋的分布特征及其季节变异   总被引：8，自引：4，他引：8  

汤毓祥 《海洋与湖沼》1996,27(4):436-444

根据１９３４－１９８８年东海水文观测资料，重点分析东海温度锋的分布特征及其季节变异，并结合近期中日黑潮合作调查研究成果，初步探讨温度锋季节变异和水团演变的关系，所得主要结论是：（１）东海不仅常年存在浙闽沿岸锋，东海北部陆架锋和黑潮锋，而且、春、夏两季，在东海南部还出现一条东海中部出架锋。（２）江海温度锋季节变化的特点是：冬季，锋的宽度和强度皆是表层最强，夏季，表层温度锋仅出现在浙江近岸小范围海域。  相似文献   

Characterization of interface roughness of rippled sand off Fort Walton Beach, Florida   总被引：1，自引：0，他引：1  

Briggs  K.B. Dajun Tang Williams  K.L. 《Oceanic Engineering, IEEE Journal of》2002,27(3):505-514

As part of the environmental characterization to model acoustic bottom scattering during the high-frequency sediment acoustics experiment (SAX99), fine-scale sediment roughness of a medium sand was successfully measured within a 600 /spl times/ 600-m area by two methods: stereo photography and a technique using a conductivity system. Areal coverage of the two methods, representing approximately 0.16 m/sup 2/ of the sea floor, was comparable, resulting in the depiction and quantification of half-meter wavelength sand ripples. Photogrammetric results were restricted to profiles digitized at 1-mm intervals; sediment conductivity results generated gridded micro-bathymetric measurements with 1- to 2-cm node spacing. Roughness power spectra give similar results in the low-spatial-frequency domains where the spectra estimated from both approaches overlap. However, spectra derived from higher resolution photogrammetric results appear to exhibit a multiple-power-law fit. Roughness measurements also indicate that spectrum changes as a function of time. Application of statistical confidence bounds on the power spectra indicates that roughness measurements separated by only 1-2 m may be spatially nonstationary.  相似文献   

南极半岛西北部海域辫螅属(水螅虫类)一新种     

唐质灿 《海洋科学集刊》1991,(32):1-6

1984-1985年，中国首次南大洋考察队“向阳红10号”调查船，在南极半岛西北部海域进行海洋综合考察时，用底栖生物拖网采获了许多水螅虫标本，经整理鉴定，发现其中有辫螅属(桧叶螅科)的一个种，形态特异，与该属的已知种均不相同，确认为新种。  相似文献