Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels   总被引：5，自引：0，他引：5  

Scholz S  Kordes C  Hamann J  Gutzeit HO 《Marine environmental research》2004,57(3):235-244

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.  相似文献   

盐渍土壤的介电特性     

Sree.  K 《海岸工程》1996,15(4):81-85

采用Ｌ波段介电探针，对沙壤，砂质粘土和粘土三种不同性质的土壤作了详尽的实验室实验，结果显示，土壤的盐度同介电常数的实无关，而同虚部相关，并随盐度的增加而增加。还表明，实数部分更多的依赖于土壤的湿度，虚数部分取决地土壤的盐度，土壤的含钠性对介电常数的实部和虚部都无影响。本研究展示了Ｌ波段对土壤盐度的微波响应。同时说明，在湿壤的条件下，采用Ｌ波段，将盐壤从钠壤中分离出来的可能性。  相似文献   

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis   总被引：3，自引：1，他引：3  

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2)

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po  相似文献   

阿拉伯海的扩张历史：某些新的控制条件     

A.K.Chaubey尹延鸿  李延成 《海洋地质前沿》1994,(1):12-13

根据海台、无震海岭．活动洋中脊以及几条破碎带，可把阿拉伯海划分成几个深海盆地．这些盆地的进化历史已从地球物理的研究中推演出来．东阿拉伯海盆(EAB)位于Laxmi-Laceadive海岭及印度的西部大陆坡之间，被认为其下伏是由裂谷和相关过程形成的过渡地壳，  相似文献   

Upper temperature tolerances of exotic brackish-water mussel, Mytilopsis leucophaeata (Conrad): an experimental study   总被引：1，自引：0，他引：1  

Rajagopal S  Van der Gaag M  Van der Velde G  Jenner HA 《Marine environmental research》2005,60(4):512-530

The dark false mussel, Mytilopsis leucophaeata is an important mussel colonising the brackish-water systems of temperate and subtropical regions. Of late it has earned notoriety as a biofouling species in industrial cooling water systems. However, there are no published data on the temperature tolerance of this species. This paper presents data on the upper temperature tolerance of this mussel from the view point of biofouling control using thermal methods. In addition to mortality, response of physiological activities such as oxygen consumption, filtration rate, foot activity and byssus thread production were also studied at temperatures varying from 5 to 35 degrees C. Experiments were also carried out to understand the effect of mussel size, breeding condition, nutritional status and acclimation conditions (temperature and salinity) on the mortality pattern. The physiological activities were significantly reduced at temperatures beyond 27.5 degrees C and ceased at 35 degrees C. In 20 mm size group mussels exposed to 37 degrees C, 50% mortality was observed after 85 min and 100% mortality after 113 min. The effect of mussel size on mortality at different temperatures was significant, with the larger size group mussels showing greater resistance. M. leucophaeata collected during the non-breeding season (December-April) were more tolerant to temperature than those collected during the breeding season (June-October). Nutritional status of the mussel had no significant influence on the thermal tolerance of the mussel: fed and starved (non-fed) mussels succumbed to temperature at comparable rates. The effect of acclimation temperature and acclimation salinity on M. leucophaeata mortality at different temperatures was significant. Survival time increased with increasing acclimation temperature and decreased with increasing salinity. In comparison with other co-occurring species such as Mytilus edulis and Dreissena polymorpha, M. leucophaeata appears to be more tolerant to high temperature stress.  相似文献   

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

An Experi mental Study on Wave-Induced Dynamic Stresses in Seabed     

S.C.Chang  L.K.Chien  J .G.Lin  Y.F.Chiu 《中国海洋工程》2006,20(1)

1 .IntroductionEvery year ,Taiwanis subjectedtotwo or three typhoonintrusions in average which always causedisasters . Most of the disasters which occur around the coastal zone are due to seabed instability andcoastal structure destruction caused bytyphoo…  相似文献   

海洋潜在的能源一天然气水合物     

HarshK．Gupta张明  姚伯初 《海洋地质》2004,(1):48-53

天然气水合物广泛分布在海洋和极地沉积物中。在这种沉积物中具备足够低的温度和足够高的压力使得甲烷气结晶变成水合物。由于水合物的广泛分布和其潜在的能源潜力—据估计其约为地球上所有化石燃料能源的两倍．引起了全世界科学界的关注。然而。至今为止对水合物的性质和分布还了解不够，还没有合适的技术能对水合物进行定量的评价。根据地形、海底温度、沉积条件以及有机碳等资料指出在印度近海具备天然气水合物形成的条件。因此有必要在印度大陆边缘系统开展地质、地球化学和地球物理分析，以识别和估算天然气水合物的储量，评价其资源潜力。类似“气烟囱”或气体溢出持征、地震剖面上的BSR、氯化物或硫化物异常都是识别水合物的有用标志。层析成像、AVO、波形反演是定量分析水合物和游离气含量的重要手段。此外，在地震方法无法识别的地方，电阻率异常也有助干天然气水合物的识别。本文给出一些划出水合物赋存区边界和定量分析水合物及其下伏游离气含量的重要研究成果和技术。  相似文献   

西南日本俯冲带的热状态“俯冲板块年龄史的影响     

Wang.  K 戴佩君 《海洋地质译丛》1996,(2):47-58

  相似文献   

欧亚冰盖的消融     

D．R．Lindstrom王贵彬  龚建明 《海洋地质前沿》1994,(7):18-19

巴巴多斯和新几内亚的海平面变化表明：在过去的15000a中曾有过两次大规模的冰川融水流．同其它环境变化(如北大西洋深水环流)相比，这两次融水流形成的时间显然有助于我们进一步了解从未次冰期到问冰期的气候变迁及新仙女木事件．  相似文献