Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

青藏高原隆升的非线性动态有限元仿真研究   总被引：7，自引：4，他引：3  

杨红心  王乘  张秋文  郑文衡 《大地测量与地球动力学》2002,22(2):75-81

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。  相似文献   

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）     

Juei-Hwa Hu  Wing-Huen Ip 《中国天文和天体物理学报》2005,5(4):356-362

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.  相似文献   

Multi-wavelength Diagnostics of Bombardment by Non-thermal Particles in Solar Flares     

Zhi Xu  Cheng Fang  Wei-Qun Can Astronomy Department of Nanjing University  Nanjing  Purple Mountain Observatory  Chinese Academy of Sciences  Nanjing 《中国天文和天体物理学报》2005,5(5):519-530

Chromospheric lines, including Ha, Lyα, Lyβand CaⅡK, CaⅡ8542, are systemically and quantitatively investigated with respect to the non-thermal excitation and ionization due to particle beam bombardment for a series of solar semi-empirical atmospheric models. As a result we propose to use the contrast in the integrated intensity of hydrogen lines to estimate the total energy flux of the bombarding beam during the solar flare impulsive phase. Partial frequency redistribution is considered in the Lyαline calculation and a smaller intensity enhancement in the Ha line-centers is found than in the previous results of Fang et al.  相似文献   

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes     

Zhang Shicui  Wu Xianhan  Zhou Jing  Wang Renxue  Wu Shangqin 《中国海洋湖沼学报》1990,8(3):263-272

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica  相似文献   

A numerical model for Type II supernovae — The collapse stage     

Cong Xu  Zong-wei Li  Yun-zao Ge 《Chinese Astronomy and Astrophysics》1985,9(2):154-158

We use the following numerical model for the collapse stage of a Type II supernova of 15 M_⊙. Our electron capture rate includes the effects of the inverse reaction and the neutron-proton mass difference. This decreases the electron density at the collapse stage and led to rather large values of the maximum inward velocity and of the corresponding mass (Umax = 3.06 × 10⁹cm/s, Mmax=0.76 M_⊙). These larger values are more favourable for the propagation of shock after the rebounce and the triggering-off of a Type-II supernova explosion. For neutrino transport, we use a leakage model and an equilibrium diffusion model, respectively, for the thin and thick stages and a grey atmosphere model to assess the effect of neutrino precipitation on the collapse. We found this effect to be small, the energy precipitation to be not more than 10^?5 the neutrino energy loss and the momentum precipitation not more than 10^?6 the gravitational acceleration.  相似文献   

Evolution of Electric Currents Associated with Two M-Class Flares     

Baolin Tan  Haisheng Ji  Guangli Huang  Tuanhui Zhou  Qiwu Song  Yu Huang 《Solar physics》2006,239(1-2):137-148

Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare.  相似文献   

Observational constraints on the radio and γ-ray emission regions of PSR B1055−52     

H. G. Wang  G. J. Qiao  R. X. Xu  Y. Liu 《Monthly notices of the Royal Astronomical Society》2006,366(3):945-952

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The formation and stability of the configuration of the planetary system HD 126611     

Zhang Xu-dong  Zhou Ji-lin 《Chinese Astronomy and Astrophysics》2006,30(4):420-430

Recent Doppler velocity measurements have revealed the existence of two planets orbiting the star HD 12661 on medium-eccentricity orbits. The inner planet has a period of 263.6 d and a mass of 2.3 Jupiter masses, and the outer planet has a period of 1444.5d and a mass of 1.57 Jupiter masses. The stability of this system requires the two planets to be in a state of mean-motion orbit resonances. By numerical method we have studied the orbit migration and stability of the system in its early ages under the action of the proto-stellar disk, and calculated the probabilities of the planets being captured into the mean -motion resonances during their migrations. It is found that at present the two planets are probably situated at the edge of the 11:2 mean-motion resonance and are in chaotic motions. This result may be helpful to clarify the arguments on the present configuration. Besides, it is indicated that very probably, after the formation of the system, the gaseous disk has almost disappeared before the planets migrated to the present configuration.  相似文献   

Observational evidence for solar coronal decimetric radio pulsations with very short periods     

Xie Rui-xiang  Wang Min  Shi Shuo-biao  Xu Chun  Li Wei-hua  Yan Yi-hua 《Chinese Astronomy and Astrophysics》2003,27(4):426-434

Using the decimetric (700–1500 MHz) radio spectrometer and the synchronous observational system with high temporal resolution at four frequencies (1420, 2130, 2840 and 4260 MHz) of Yunnan Observatory, two rare events were observed on 2001 June 24 and 1990 July 30. The former was a small radio burst exhibiting pulsations with short periods (about 29, 40 and 100 ms) in the impulsive phase. The latter was a large radio burst, which at 2840 MHz produced radio pulsations with period of about 30 ms. This paper focuses on pulsations with very short periods in the range of 29–40 ms. The mechanism of generation of such pulsations may be modulation of radio radiation by the periodic trains of whistler packets originating in unstable regions of the corona. Alternatively, these pulsations can be attributed to wave-wave non-linear interactions of electrostatic upper hybrid waves driven by beams of precipitating electrons in flaring loops.  相似文献