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81.
Katayama Y Oura T Iizuka M Orita I Cho KJ Chung IY Okada M 《Marine pollution bulletin》2003,47(1-6):85-90
Effects of spilled oil on microbial communities in tidal flats were examined by use of a simulator for a tidal flat ecosystem. The simulator is composed of a wave generator, a tide control device, and a tidal flat. Sediment for the tidal flat was obtained at a natural tidal flat in Hiroshima Bay, Japan. After stabilizing the benthic organisms, fuel oil C was added to the surface of the flat at 1 lm(-2). Although the total number of micro-organisms remained at 1.5-3.5 x 10(9) cells g(-1) dry sediment irrespective of the addition of oil, bacterial communities which were analyzed based on the 16S rDNA showed clear changes after the addition of fuel oil C and after a subsequent recovery period. Bacterial colonies were randomly isolated from the oil-supplemented sediment during the experiments, and the isolates were examined for susceptibility to hydrocarbons in order to screen the oil-susceptible bacteria. The proportion of oil-susceptible bacteria in the isolates decreased with the addition of the oil. Oil-susceptible bacteria showed an inability to assimilate petroleum compounds as well as an inhibition of growth. The possibility of using oil-susceptible bacteria as an indicator of bioremediation in tidal flats was discussed. 相似文献
82.
Experimental batch and miscible-flow cultures were studied in order to determine the mechanistic pathways of microbial Fe(III)
respiration in ferruginous smectite clay, NAu-1. The primary purpose was to resolve if alteration of smectite and release
of Fe precedes microbial respiration. Alteration of NAu-1, represented by the morphological and mineralogical changes, occurred
regardless of the extent of microbial Fe(III) reduction in all of our experimental systems, including those that contained
heat-killed bacteria and those in which O2, rather than Fe(III), was the primary terminal electron acceptor. The solid alteration products observed under transmission
electron microscopy included poorly crystalline smectite with diffuse electron diffraction signals, discrete grains of Fe-free
amorphous aluminosilicate with increased Al/Si ratio, Fe-rich grains, and amorphous Si globules in the immediate vicinity
of bacterial cells and extracellular polymeric substances. In reducing systems, Fe was also found as siderite. The small amount
of Fe partitioned to the aqueous phase was primarily in the form of dissolved Fe(III) species even in the systems in which
Fe(III) was the primary terminal electron acceptor for microbial respiration. From these observations, we conclude that microbial
respiration of Fe(III) in our laboratory systems proceeded through the following: (1) alteration of NAu-1 and concurrent release
of Fe(III) from the octahedral sheets of NAu-1; and (2) subsequent microbial respiration of Fe(III). 相似文献
83.
Natural Hazards - Although children are exposed to a high mortality risk during disasters, what determines their disaster response, especially during earthquakes, remains largely unexplored. The... 相似文献