Transposable genetic elements inSpirulina and potential applications for genetic engineering     

Kojima  Hiroyuki  Song  Qin  Ajith Kumar  Thankappan  Kawata  Yoshikazu  Yano  Shin-ichi 《中国海洋湖沼学报》1998,16(1):30-39

Transposable elements in cyanobacteria are briefly reviewed. Evidence is presented to show that transposable elements inSpirulina platensis is actually reflected on the phenotype change, i e., helical to straight filaments. Transposition intermediates of DNA were isolated from the extrachromosome and the transposition was related to helical variations inSpirulina. Uses of transposable elements for microalgal recombination are discussed based on the transposition mechanism.

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A practical model to estimate photosynthetically active radiation using general meteorological elements in a temperate humid area and comparison among models     

Yasuko Mizoguchi  Yukio Yasuda  Yoshikazu Ohtani  Tsutomu Watanabe  Yuji Kominami  Katsumi Yamanoi 《Theoretical and Applied Climatology》2014,115(3-4):583-589

Although accurately evaluating photosynthetically active radiation is important, much effort is required to measure this radiation using a quantum sensor. We develop a new model that makes estimates using only general meteorological data—solar radiation, atmospheric pressure, air temperature, and relative humidity. Root mean square deviations for eight datasets at five sites in Japan were smaller than 5.2 %, similar to error in other studies and to individual differences of quantum sensors. Most root mean square deviations of nine previous models and our eight datasets are larger than that of the new estimation model, which performed well. This suggests that the model is useful for estimating photosynthetically active radiation in a temperate, humid area of Japan.  相似文献   

Hydrogen sulfide and organic carbon at the sediment–water interface in coastal brackish Lake Nakaumi, SW Japan     

Saburo Sakai  Masaru Nakaya  Yoshikazu Sampei  David L. Dettman  Katsumi Takayasu 《Environmental Earth Sciences》2013,68(7):1999-2006

The relationship among H₂S, total organic carbon (TOC), total sulfur (TS) and total nitrogen contents of surface sediments (0–1 cm) was examined to quantify the relationship between H₂S concentrations and TOC content at the sediment water interface in a coastal brackish lake, Nakaumi, southwest Japan. In this lake, bottom water becomes anoxic during summer due to a strong halocline. Lake water has ample dissolved SO₄ ^2? and the surface sediments are rich in planktic organic matter (C/N 7–9), which is highly reactive in terms of sulfate reduction. In this setting the amount of TOC should be a critical factor regulating the activity of sulfate reduction and H₂S production. In portions of the lake where sediment TOC content is less than 3.5 %, H₂S was very low or absent in both bottom and pore waters. However, in areas with TOC >3.5 %, H₂S was correlated with TOC content (pore water H₂S (ppm) = 13.9 × TOC (%) ? 52.1, correlation coefficient: 0.72). H₂S was also present in areas with sediment TS above 1.2 % (present as iron sulfide), which suggests that iron sulfide formation is tied to the amount of TOC. Based on this relationship, H₂S production has progressively increased after the initiation of land reclamation projects in Lake Nakaumi, as the area of sapropel sediments has significantly increased. This TOC–H₂S relationship at sediment–water interface might be used to infer H₂S production in brackish–lagoonal systems similar to Lake Nakaumi, with readily available SO₄ ^2? and reactive organic matter.  相似文献   

Permian Peri‐glacial Deposits from Central Mongolia in Central Asian Orogenic Belt: A Possible Indicator of the Capitanian Cooling Event     

Tatsuya Fujimoto  Shigeru Otoh  Yuji Orihashi  Takafumi Hirata  Takaomi D. Yokoyama  Masanori Shimojo  Yoshikazu Kouchi  Hokuto Obara  Yasuo Ishizaki  Kazuhiro Tsukada  Toshiyuki Kurihara  Manchuk Nuramkhan  Sersmaa Gonchigdorj 《Resource Geology》2012,62(4):408-422

A dropstone‐bearing, Middle Permian to Early Triassic peri‐glacial sedimentary unit was first discovered from the Khangai–Khentei Belt in Mongolia, Central Asian Orogenic Belt. The unit, Urmegtei Formation, is assumed to cover the early Carboniferous Khangai–Khentei accretionary complex, and is an upward‐fining sequence, consisting of conglomerates, sandstones, and varved sandstone and mudstone beds with granite dropstones in ascending order. The formation was cut by a felsic dike, and was deformed and metamorphosed together with the felsic dike. An undeformed porphyritic granite batholith finally cut all the deformed and metamorphosed rocks. LA‐ICP‐MS, U–Pb zircon dating has revealed the following ²⁰⁶Pb/²³⁸U weighted mean igneous ages: (i) a granite dropstone in the Urmegtei Formation is 273 ± 5 Ma (Kungurian of Early Permian); (ii) the deformed felsic dike is 247 ± 4 Ma (Olenekian of Early Triassic); and (iii) the undeformed granite batholith is 218 ± 9 Ma (Carnian of Late Triassic). From these data, the age of sedimentation of the Urmegtei Formation is constrained between the Kungurian and the Olenekian (273–247 Ma), and the age of deformation and metamorphism is constrained between the Olenekian and the Carnian (247–218 Ma). In Permian and Triassic times, the global climate was in a warming trend from the Serpukhovian (early Late Carboniferous) to the Kungurian long and severe cool mode (328–271 Ma) to the Roadian to Bajocian (Middle Jurassic) warm mode (271–168 Ma), with an interruption with the Capitanian Kamura cooling event (266–260 Ma). The dropstone‐bearing strata of the Urmegtei Formation, together with the glacier‐related deposits in the Verkhoyansk, Kolyma, and Omolon areas of northeastern Siberia (said to be of Middle to Late Permian age), must be products of the Capitanian cooling event. Although further study is needed, the dropstone‐bearing strata we found can be explained in two ways: (i) the Urmegtei Formation is an autochthonous formation indicating a short‐term expansion of land glacier to the central part of Siberia in Capitanian age; or (ii) the Urmegtei Formation was deposited in or around a limited ice‐covered continent in northeast Siberia in the Capitanian and was displaced to the present position by the Carnian.  相似文献   

Shaking table tests of steel frame with superelastic Cu–Al–Mn SMA tension braces          下载免费PDF全文

Yoshikazu Araki  Kshitij C. Shrestha  Nao Maekawa  Yuji Koetaka  Toshihiro Omori  Ryosuke Kainuma 《地震工程与结构动力学》2016,45(2):297-314

Shaking table tests are performed on a one‐bay one‐story steel frame with superelastic Cu–Al–Mn shape memory alloy (SMA) tension braces. The frame is subjected to a series of scaled ground motions recorded during the 1995 Kobe earthquake, Japan. The test results demonstrate that the SMA braces are effective to prevent residual deformations and pinching. It is also shown that the time history responses observed from the shaking table tests agree well with the numerical predictions using a rate‐independent piecewise‐linear constitutive model calibrated to the quasi‐static component tests of the SMA braces. This suggests that the loading rate dependence of Cu–Al–Mn SMAs as well as the modeling error due to the piecewise linear approximation can be neglected in capturing the global response of the steel frame. Numerical simulations under a suite of near‐fault ground motion records are further performed using the calibrated analytical models to demonstrate the effectiveness of the SMA braces when the variability of near‐fault ground motions is taken into account. A stopper, or a deformation restraining device, is also proposed to prevent premature fracture of SMA bars in unexpectedly large ground motions while keeping the self‐centering capability in moderate to large ground motions. The effectiveness of the stopper is also demonstrated in the quasi‐static component and shaking table tests. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant     

Yano  Shin-ichi  Kawata  Yoshikazu  Kojima  Hiroyuki 《中国海洋湖沼学报》1998,16(1):105-108

A unicellular cyanobacteriumSynechococcus sp. strain PCC 7002 was transformed with plasmid pQL1, on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were encoded. The transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This result indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase, and accumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmotic shocks wihout, impairing cell viability. On the other hand, most of the β-galactosidase remained in cytoplasm under the osmotic shock.

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Mira variables in the Galactic bulge with OGLE-II data     

Noriyuki Matsunaga  Hinako Fukushi  Yoshikazu Nakada 《Monthly notices of the Royal Astronomical Society》2005,364(1):117-125

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U–Pb zircon age from the radiolarian‐bearing Hitoegane Formation in the Hida Gaien Belt,Japan     

Manchuk Nuramkhaan  Toshiyuki Kurihara  Kazuhiro Tsukada  Yoshikazu Kochi  Hokuto Obara  Tatsuya Fujimoto  Yuji Orihashi  Koshi Yamamoto 《Island Arc》2013,22(4):494-507

The dating of radiolarian biostratigraphic zones from the Silurian to Devonian is only partially understood. Dating the zircons in radiolarian‐bearing tuffaceous rocks has enabled us to ascribe practical ages to the radiolarian zones. To extend knowledge in this area, radiometric dating of magmatic zircons within the radiolarian‐bearing Hitoegane Formation, Japan, was undertaken. The Hitoegane Formation is mainly composed of alternating beds of tuffaceous sandstones, tuffaceous mudstones and felsic tuff. The felsic tuff and tuffaceous mudstone yield well‐preserved radiolarian fossils. Zircon grains showing a U–Pb laser ablation–inductively coupled plasma–mass spectrometry age of 426.6 ± 3.7 Ma were collected from four horizons of the Hitoegane Formation, which is the boundary between the Pseudospongoprunum tauversi to Futobari solidus–Zadrappolus tenuis radiolarian assemblage zones. This fact strongly suggests that the boundary of these assemblage zones is around the Ludlowian to Pridolian. The last occurrence of F. solidus is considered to be Pragian based on the reinterpretation of a U–Pb sensitive high mass‐resolution ion microprobe (SHRIMP) zircon age of 408.9 ± 7.6 Ma for a felsic tuff of the Kurosegawa belt, Southwest Japan. Thus the F. solidus–Z. tenuis assemblage can be assigned to the Ludlowian or Pridolian to Pragian. The present data also contribute to establishing overall stratigraphy of the Paleozoic rocks of the Fukuji–Hitoegane area. According to the Ordovician to Carboniferous stratigraphy in this area, Ordovician to Silurian volcanism was gradually reduced to change the sedimentary environment into a tropical lagoon in the early Devonian. And the quiet Carboniferous environment was subsequently interrupted, throwing it once more into the volcanic conditions in the Middle Permian.  相似文献   

A numerical investigation of eddy-induced chlorophyll bloom in the southeastern tropical Indian Ocean during Indian Ocean Dipole—2006     

Iskhaq Iskandar  Hideharu Sasaki  Yoshikazu Sasai  Yukio Masumoto  Keisuke Mizuno 《Ocean Dynamics》2010,60(3):731-742

An eddy-resolving coupled physical–biological model is used to study the effect of cyclonic eddy in enhancing offshore chlorophyll-a (Chl-a) bloom in the southeastern tropical Indian Ocean during boreal summer–fall 2006. The results demonstrate that the offshore Chl-a blooms are markedly coincident with the high eddy kinetic energy. Moreover, the vertical variations in Chl-a, nitrate, temperature, and mixed-layer depth (MLD) strongly imply that the cyclonic eddies induce surface Chl-a bloom through the injection of nutrient-rich water into the upper layer. Interestingly, we found that the surface bloom only occurs when the deep Chl-a maximum is located within the MLD. On the other hand, the response of subsurface Chl-a to the eddy pumping is remarkable, although it is hardly observable at the surface.  相似文献   

Growth of twinned magnetic crystals in talc and talc-magnesite rock at Torika,Nagasaki Prefecture,Japan     

Reiko Iwasaki  Yoshikazu Aoki 《Lithos》1983,16(2):125-133

Spinel-type twins of magnetite in a talc rock and a talk-magnesite rock at Torika, Nagasaki Prefecture, Japan have been studied by means of optical microscopy, differential interference microscopy, microprobe analysis and size distribution analysis. The growth processes are discussed in detail. The number of magnetite twins in the talc-magnesite rock is larger than in the talc rock, and the percentage of the frequency number increases rapidly with increase in grain size. It is suggested that magnetite crystals in the talc-magnesite rock have grown by rapid crystallization in the earlier stage and by a layer-growth mechanism in the later stage in the carbonation-dehydration reaction that occurred within the rock. As large number of twin nuclei formed by rapid crystallization in the earlier stage of magnetite formation. As the dehydration reaction proceeded, the number of twin nuclei gradually decreased with the lowering of supersaturation by addition of some water. Magnetite crystals in the talc rock formed after talc crystallization by silicification and dehydration reactions in the process of steatitization. The twin nuclei here must have formed with difficulty, governed by the distribution of talc which crystallized before the magnetite and at relatively low supersaturation.  相似文献