Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant     

Yano  Shin-ichi  Kawata  Yoshikazu  Kojima  Hiroyuki 《中国海洋湖沼学报》1998,16(1):105-108

A unicellular cyanobacteriumSynechococcus sp. strain PCC 7002 was transformed with plasmid pQL1, on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were encoded. The transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This result indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase, and accumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmotic shocks wihout, impairing cell viability. On the other hand, most of the β-galactosidase remained in cytoplasm under the osmotic shock.

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Decreasing precipitation extremes at higher temperatures in tropical regions     

Eduardo Eiji Maeda  Nobuyuki Utsumi  Taikan Oki 《Natural Hazards》2012,64(1):935-941

It has been often accepted that rising troposphere temperatures will lead to higher precipitation intensities. This argument is based on the Clausius?CClapeyron (C?CC) relation, which indicates an increase in atmospheric moisture storage capacity of approximately 7?% K^?1. However, recent studies carried out in mid-latitude regions indicate that changes in precipitation intensity as a function of temperature do not necessarily follow the C?CC relation. This study aimed to evaluate the correlation between precipitation extremes and temperature in tropical regions, using measured data obtained at low latitude ranges over Brazil. The results indicate that, at daily timescale, the C?CC relation alone is unlikely to explain the relation between precipitation extremes and temperatures in tropical regions. Additional aspects, such as moisture availability and the duration of precipitation events, should be further analyzed to allow a comprehensive understanding of the relationship between temperature and precipitation intensity. Moreover, we show that in tropical regions, higher temperatures may reduce the magnitude of extreme precipitation events at daily timescales, independent of the season of the year.  相似文献   

Mira variables in the Galactic bulge with OGLE-II data     

Noriyuki Matsunaga  Hinako Fukushi  Yoshikazu Nakada 《Monthly notices of the Royal Astronomical Society》2005,364(1):117-125

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U–Pb zircon age from the radiolarian‐bearing Hitoegane Formation in the Hida Gaien Belt,Japan     

Manchuk Nuramkhaan  Toshiyuki Kurihara  Kazuhiro Tsukada  Yoshikazu Kochi  Hokuto Obara  Tatsuya Fujimoto  Yuji Orihashi  Koshi Yamamoto 《Island Arc》2013,22(4):494-507

The dating of radiolarian biostratigraphic zones from the Silurian to Devonian is only partially understood. Dating the zircons in radiolarian‐bearing tuffaceous rocks has enabled us to ascribe practical ages to the radiolarian zones. To extend knowledge in this area, radiometric dating of magmatic zircons within the radiolarian‐bearing Hitoegane Formation, Japan, was undertaken. The Hitoegane Formation is mainly composed of alternating beds of tuffaceous sandstones, tuffaceous mudstones and felsic tuff. The felsic tuff and tuffaceous mudstone yield well‐preserved radiolarian fossils. Zircon grains showing a U–Pb laser ablation–inductively coupled plasma–mass spectrometry age of 426.6 ± 3.7 Ma were collected from four horizons of the Hitoegane Formation, which is the boundary between the Pseudospongoprunum tauversi to Futobari solidus–Zadrappolus tenuis radiolarian assemblage zones. This fact strongly suggests that the boundary of these assemblage zones is around the Ludlowian to Pridolian. The last occurrence of F. solidus is considered to be Pragian based on the reinterpretation of a U–Pb sensitive high mass‐resolution ion microprobe (SHRIMP) zircon age of 408.9 ± 7.6 Ma for a felsic tuff of the Kurosegawa belt, Southwest Japan. Thus the F. solidus–Z. tenuis assemblage can be assigned to the Ludlowian or Pridolian to Pragian. The present data also contribute to establishing overall stratigraphy of the Paleozoic rocks of the Fukuji–Hitoegane area. According to the Ordovician to Carboniferous stratigraphy in this area, Ordovician to Silurian volcanism was gradually reduced to change the sedimentary environment into a tropical lagoon in the early Devonian. And the quiet Carboniferous environment was subsequently interrupted, throwing it once more into the volcanic conditions in the Middle Permian.  相似文献   

A numerical investigation of eddy-induced chlorophyll bloom in the southeastern tropical Indian Ocean during Indian Ocean Dipole—2006     

Iskhaq Iskandar  Hideharu Sasaki  Yoshikazu Sasai  Yukio Masumoto  Keisuke Mizuno 《Ocean Dynamics》2010,60(3):731-742

An eddy-resolving coupled physical–biological model is used to study the effect of cyclonic eddy in enhancing offshore chlorophyll-a (Chl-a) bloom in the southeastern tropical Indian Ocean during boreal summer–fall 2006. The results demonstrate that the offshore Chl-a blooms are markedly coincident with the high eddy kinetic energy. Moreover, the vertical variations in Chl-a, nitrate, temperature, and mixed-layer depth (MLD) strongly imply that the cyclonic eddies induce surface Chl-a bloom through the injection of nutrient-rich water into the upper layer. Interestingly, we found that the surface bloom only occurs when the deep Chl-a maximum is located within the MLD. On the other hand, the response of subsurface Chl-a to the eddy pumping is remarkable, although it is hardly observable at the surface.  相似文献   

In situ X-ray observations of the decomposition of brucite and the graphite–diamond conversion in aqueous fluid at high pressure and temperature     

T. Okada  W. Utsumi  H. Kaneko  M. Yamakata  O. Shimomura 《Physics and Chemistry of Minerals》2002,29(7):439-445

 An experimental technique to make real-time observations at high pressure and temperature of the diamond-forming process in candidate material of mantle fluids as a catalyst has been established for the first time. In situ X-ray diffraction experiments using synchrotron radiation have been performed upon a mixture of brucite [Mg(OH)₂] and graphite as starting material. Brucite decomposes into periclase (MgO) and H₂O at 3.6 GPa and 1050 °C while no periclase is formed after the decomposition of brucite at 6.2 GPa and 1150 °C, indicating that the solubility of the MgO component in H₂O greatly increases with increasing pressure. The conversion of graphite to diamond in aqueous fluid has been observed at 7.7 GPa and 1835 °C. Time-dependent X-ray diffraction profiles for this transformation have been successfully obtained. Received: 17 July 2001 / Accepted: 18 February 2002  相似文献   

Growth of twinned magnetic crystals in talc and talc-magnesite rock at Torika,Nagasaki Prefecture,Japan     

Reiko Iwasaki  Yoshikazu Aoki 《Lithos》1983,16(2):125-133

Spinel-type twins of magnetite in a talc rock and a talk-magnesite rock at Torika, Nagasaki Prefecture, Japan have been studied by means of optical microscopy, differential interference microscopy, microprobe analysis and size distribution analysis. The growth processes are discussed in detail. The number of magnetite twins in the talc-magnesite rock is larger than in the talc rock, and the percentage of the frequency number increases rapidly with increase in grain size. It is suggested that magnetite crystals in the talc-magnesite rock have grown by rapid crystallization in the earlier stage and by a layer-growth mechanism in the later stage in the carbonation-dehydration reaction that occurred within the rock. As large number of twin nuclei formed by rapid crystallization in the earlier stage of magnetite formation. As the dehydration reaction proceeded, the number of twin nuclei gradually decreased with the lowering of supersaturation by addition of some water. Magnetite crystals in the talc rock formed after talc crystallization by silicification and dehydration reactions in the process of steatitization. The twin nuclei here must have formed with difficulty, governed by the distribution of talc which crystallized before the magnetite and at relatively low supersaturation.  相似文献   

Essential cellular modules for the proliferation of the primitive cell     

Koichi Higashi  Yoshikazu Kawai  Tomoya Baba  Ken Kurokawa  Taku Oshima 《地学前缘(英文版)》2018,9(4):1155-1161

Bacteria appeared early in the evolution of cellular life on planet Earth, and therefore the universally essential genes or biological pathways found across bacterial domains may represent fundamental genetic or cellular systems used in early life. The essential genes and the minimal gene set required to support bacterial life have recently been experimentally and computationally identified. It is, however,still hard to estimate the ancient genes present in primitive cells compared to the essential genes in contemporary bacteria, because we do not know how ancestral primitive cells lived and proliferated, and therefore cannot directly evaluate the essentiality of the genes in ancestral primitive cells. The cell wall is normally essential for bacterial proliferation and cellular division of walled bacterial cells is normally highly controlled by the essential FtsZ cell division machinery. But, bacteria are capable of reverting to their cell wall deficient ancestral form, called the "L-form". Unlike "normal" cells, L-forms divide by a simple physical mechanism based on the effects of membrane dynamics, suggesting a mode of primitive proliferation before the appearance of the cell wall. In this review, we summarize the experimental and computational investigations of minimal gene sets and discuss the minimal cellular modules required to support the proliferation of primitive cells, based on L-form proliferation.  相似文献   

Kinetics of the graphite–diamond transformation in aqueous fluid determined by in-situ X-ray diffractions at high pressures and temperatures     

T.?OkadaEmail author  W.?Utsumi  H.?Kaneko  V.?Turkevich  N.?Hamaya  O.?Shimomura 《Physics and Chemistry of Minerals》2004,31(4):261-268

The graphite-diamond transformation was investigated by in situ time-resolved X-ray diffraction experiments using aqueous fluid containing dissolved MgO as the diamond-forming catalyst under conditions of 6.6–8.9 GPa and 1400–1835 °C. The transformed volume fractions of diamond as a function of time under various pressure-temperature conditions were obtained and analyzed using the JMAK rate equation. Variations in the nucleation and growth processes during diamond formation as a function of pressure and temperature were clarified.  相似文献   

Vertical vibration isolator having piecewise‐constant restoring force     

Yoshikazu Araki  Takehiko Asai  Takeshi Masui 《地震工程与结构动力学》2009,38(13):1505-1523

We present a vertical vibration isolator having a piecewise‐constant restoring force, which belongs to a class of passive and nonlinear vibration isolators. In vertical vibration isolation, direct use of low‐stiffness elements leads to unacceptably large deformations due to self‐weight. To overcome the difficulty, we apply a combination of constant‐force springs, each of which sustains a constant load regardless of its stretch. By arranging the constant‐force springs, so that the isolator has a piecewise‐constant restoring force, we alleviate the problem of the excessive deformation caused by self‐weight, provide stability at the static equilibrium state along with the self‐centering capability, and realize a large stroke while keeping the mechanism simple and compact. Further, we attempt to limit the response acceleration within a tolerance regardless of the frequency spectrum and the magnitude of earthquake ground motions. We demonstrate the effectiveness of the present isolator through shaking table tests and numerical simulations. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献