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Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis 总被引:2,自引:0,他引:2
Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping. 相似文献
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Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were
detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three
linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis
(ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method
in microsatellite genotyping.
Supported by the National Natural Science Foundation of China (NO.40730845, 39825121) 相似文献
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皱纹盘鲍表型性状与肌肉重的相关与通径分析 总被引:2,自引:0,他引:2
选取日本岩手县×中国大连獐子岛的一个杂交家系,随机测定132尾三年龄皱纹盘鲍(Haliotis discus hannai Ino)的壳长(x1)、壳宽(x2)、总重(x3)、壳重(x4)、软体部重(x5)及肌肉重(y)6个指标,计算各性状间的相关系数,采用通径分析方法计算以表型性状为自变量,肌肉重为依变量的通径系数、决定系数,对各性状的影响大小进行剖分,明确影响皱纹盘鲍肌肉重的主要表型性状,为皱纹盘鲍选育提供理论依据和理想的测度指标。结果表明:所测各表型性状与肌肉重之间的相关系数均达到极显著水平(P0.01);软体部重对肌肉重的直接影响最大(为0.512),其次为总重,对肌肉重的直接影响为0.484,二者差异不显著(P0.05),其他性状对肌肉重的直接影响均不大。剔除通径系数检验不显著的自变量,利用逐步回归的方法,建立以肌肉重为依变量的最优多元线性回归方程为:y=-0.381+0.239x3+0.342x5,其调整决定系数为0.989,表明所选性状是影响肌肉重的主要性状。 相似文献
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贝类多倍体技术在细胞 染色体育种技术体系中占有重要地位 ,而倍性的鉴定是多倍体育种中的一个重要环节。以往人们主要用细胞染色体计数技术进行倍性的检测[4]。但该技术操作复杂 ,技术要求高且不稳定 ,耗时长 ,而且在大多情况下还要杀死取样对象或对其造成较大的生理伤害。由于在杂交多倍体育种中四倍体的珍贵性 ,需要保持其健康存活状态。因此 ,其快速的活体倍性检测技术的重要性尤显突出。Allen等1983年用流式细胞法 (FlowCytometry)测定了经人工三倍体诱导的鱼类和贝类的倍性 ,取得了较好的结果。周岭华等[… 相似文献
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从以日本野生皱纹盘鲍(Haliotis discus hannui Ino)为亲本进行群体繁殖所得人工群体的子1代中分别选择大(BI)、中(ME)、小(SM)等3个子群体各20个个体,进行RAPD分析。用11条随机引物及7个双引物组合共扩增出153条DNA片段,其中总群体多态性片段数为110条,多态性片段的比例为72.37%。根据扩增片段的共享度用TFPGA软件进行计算,结果表明:(1)SM与ME的遗传距离较近,而与BI的遗传距离相差较大;(2)子群体间遗传分化系数为7.64%,表明各个子群体间在遗传上存在一定的差异,对子群体进行选择将有一定的意义;(3)各子群体表现出了丰富的遗传变异,子群体BI、ME、SM的遗传多样性分别为0.2063、0.2358和0.2363,表明BI子群体具有相对较高的纯合度,在选择育种中有较高价值。 相似文献
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不规则物体体积测量的传统方法有排液法、称重法和排气法等,不仅操作繁琐,而且准确度及精确度低。本文首次提出了排水称重法,利用该方法能够更加简单、快速、准确地测量出物体的体积和质量。用此法对不同海区26个采样点的1100个牡蛎贝壳密度进行了测定分析,结果表明:大部分牡蛎的左壳密度显著小于右壳密度(P0.05);对于不同海区间的牡蛎,贝壳密度存在极显著的差异(P0.001);分布海区相近的牡蛎间,壳的密度也趋于相近;受环境压力相对较大的潮间带上部的牡蛎,贝壳密度也相对较大。本研究为剖析牡蛎贝壳形成机制以及温度、盐度和海洋酸化等环境因素对牡蛎贝壳形成的影响等研究提供了实验数据和参考。 相似文献
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Yield trait improvement of bay scallops following complete diallel crosses between different scallop stocks 总被引:2,自引:0,他引:2
This study describes the complete diaUel hybridization between newly introduced bay scallop stock (W) from Canada and local commercial stock (D) grown under laboratory conditions, in China. Larval survival and growth during all life stages (larvae, spat, and adult) were compared among hybrid (DW, WD) and purebred (DD, WW) populations. Significant heterosis was detected for survival during the larval stage (〉 1% of the mid-parent values). The mean heterosis (Hm) varied in growth throughout the life span. More than 50% of the Hm values were positive and negative in the DW and WD groups, respectively. The influence of maternal effects and mating types (intrapopulation vs. interpopulation crosses) on growth for all life stages was not consistent. Larval survival did not differ significantly (P〉0.05) with maternal effect or mating type. In the harvest stage, shell length (SL), shell height (SH), shell width (SW), and total weight (TW) were larger in the hybrid compared with the inbred groups. Positive Hm values were observed in SL (1.5%), SW (5.8%), and TW (12.3%), and were more significant in the DW groups (6.1%, 4.5%, 6.8%, and 27.2%). These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study. 相似文献