The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta     

Isa D.L. Gomes  Marco F.L. Lemos  Amadeu M.V.M. Soares  Carlos Barata  Melissa Faria 《Marine pollution bulletin》2014

The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta’s ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC₅₀ = 60.72 nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.  相似文献   

Short‐ and Long‐Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata          下载免费PDF全文

Manuela D. Machado  Eduardo V. Soares 《洁净——土壤、空气、水》2016,44(11):1578-1583

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Microbiological monitoring of bivalves from the Ria Formosa Lagoon (south coast of Portugal): a 20 years of sanitary survey   总被引：2，自引：0，他引：2  

Almeida C  Soares F 《Marine pollution bulletin》2012,64(2):252-262

The microbiological pollution of coastal waters is a major problem, especially in shellfish areas. This article shows the faecal contamination in bivalves from the Ria Formosa Lagoon (south coast of Portugal) along 20 years (1990-2009). The highest values of Escherichia coli in bivalves were obtained during the 90s, related with the discharge of untreated wastewaters and agricultural runoff. In the 2000s contamination levels decreased, with 83% of the population already served by new or remodelled sewage treatment plants. The highest levels were found in bivalves close to the largest city, where punctual and diffuse contamination sources still exist. Bivalves from the less impacted site showed the lowest contamination, an area with more water renewal. Seasonally, the highest levels were in autumn and winter, due to the runoff of waters from rainfall. These were opposite to those in spring and summer, when the highest temperatures and salinity showed a bactericidal effect.  相似文献   

Ras gene in marine mussels: a molecular level response to petrochemical exposure     

Lima I  Peck MR  Rendón-Von Osten J  Soares AM  Guilhermino L  Rotchell JM 《Marine pollution bulletin》2008,56(4):633-640

Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species.  相似文献   

On the Climate Impact of the Local Circulation in the Itaipu Lake Area   总被引：1，自引：0，他引：1  

Sônia M. S. Stivari  Amauri P. De Oliveira  Jacyra Soares 《Climatic change》2005,72(1-2):103-121

The impact of the Itaipu Lake on the climate and local circulation is investigated here using the meteorological information available in the area. The Itaipu Lake is an artificial water reservoir of 1460 km² (approximately 170 km by 7.5 km), formed in 1982 as part of the Itaipu Power Plant. It is situated on the Brazil-Paraguay frontier, in the central portion of Parana River Valley. The analysis of the available meteorological data (air and water temperatures, air relative humidity, precipitation and radiosonde soundings) provides observational evidences supporting the hypothesis that the Itaipu Lake presence has an important impact in the local circulation, inducing a local circulation with lake breeze characteristics showing horizontal wind divergence over the lake during daytime and convergence during nighttime. From the regional point of view, the Itaipu Lake formation has reduced the thermal amplitude of the diurnal air temperature cycle. The precipitation data, investigated here, has not indicated any systematic effect associated to the lake formation. The reason for the apparent inconsistency is that others phenomena (e.g., valley-mountain circulation and El Niño events) could be masking the impact of the lake formation on the rain deficit in the region.  相似文献   

Geostatistical Seismic Inversion with Self-Updating of Local Probability Distributions     

Azevedo  Leonardo  Narciso  João  Nunes  Rúben  Soares  Amílcar 《Mathematical Geosciences》2021,53(5):1073-1093

Mathematical Geosciences - Three-dimensional subsurface elastic models inverted from seismic reflection data are the basis of the geo-modeling workflow. These models are often used to predict the...  相似文献   

Geostatistics for Environmental Applications     

Maria João Pereira  Amilcar Soares 《Mathematical Geosciences》2018,50(2):123-125

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Vertical deformation partitioning across a collapsing large and hot orogen     

F. A. Temporim  Ricardo Ivan Ferreirae da Trindade  Marcos Egydio-Silva  Tiago Valim Angelo  Eric Tohver  Caroline Cibele Soares  Lucas Pequeno Gouvêa  Julio Cesar Mendes  Silvia Regina Medeiros  Antônio Carlos Pedrosa-Soares  Gabriel Gomes Silva 《地学学报》2023,35(1):23-31

Featuring 3 000-km-long large and hot orogen, the Mantiqueira Province provides a rare opportunity to study the process of gravitational collapse at mid to deep crustal levels. Distinct but contemporary (~500 Ma) post-collisional intrusions show structures and anisotropy of magnetic susceptibility (AMS) fabrics related to their emplacements, recording different flow patterns. In southern deep-seated intrusions, ellipsoidal-shaped roots with gabbroic-to-hybrid cores surrounded by granitic rocks show concentric patterns of AMS fabrics that cut across the NE-trending regional foliation. In contrast, northern intrusions, exposed as the upper sections of batholith-size bodies of coarse-grained granite emplaced at the shallow to mid-crust, show general NS-trending magnetic fabrics roughly parallel to strike of the orogen and the regional foliation of host rocks. These contrasting magnetic patterns from shallow to deeper crust suggest vertical magma migration from the overthickened orogenic core to be emplaced across its thinner stretched flanks during the gravitational collapse of the orogenic edifice.  相似文献