Zinc concentration effect at the organismal,cellular and subcellular levels in the eastern oyster     

Meiller JC  Bradley BP 《Marine environmental research》2002,54(3-5):401-404

The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc.  相似文献   

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants   总被引：1，自引：0，他引：1  

Stentiford GD  Longshaw M  Lyons BP  Jones G  Green M  Feist SW 《Marine environmental research》2003,55(2):137-159

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.  相似文献   

Phototoxicity of pyrene and benzo[a]pyrene to embryo-larval stages of the Pacific oyster Crassostrea gigas     

Lyons BP  Pascoe CK  McFadzen IR 《Marine environmental research》2002,54(3-5):627-631

There is a growing body of evidence to suggest that certain polycyclic aromatic hydrocarbons (PAHs) pose a greater hazard to aquatic organisms than previously demonstrated, due to their potential to cause photo-induced toxicity when exposed to ultraviolet (UV) radiation. The consequences of photo-induced toxicity are reported here for embryo-larval stages of the pacific oyster Crassostrea gigas, following exposure to pyrene and benzo[a]pyrene. During laboratory investigations, significant increases in toxicity were observed in the presence of environmentally attainable levels of UV-radiation, compared with embryos exposed to PAH alone, at levels previously deemed to have little acute biological effect. The phototoxicity of pyrene and benzo[a]pyrene completely inhibited the development to the D-shell larval stage when embryos were simultaneously exposed to 5 microg l(-1) PAH and ultraviolet light (UVB = 6.3 +/- 0.1 microW/cm2 and UVA = 456.2 +/- 55 microW/cm2). A linear relationship was also demonstrated for benzo[a]pyrene phototoxicity with decreasing UV light intensity.  相似文献   

On the applicability of KirchofPs law and the principle of heat balance in thermal infrared remote sensing:A non-isothermal system     

ZHANG Renhua  LI Zhaoliang  SUN Xiaomin  ZHU Zhilin & WANG Weimin Institute of Geographical Sciences  Natural Resources Research  Chinese Academy of Sciences  Beijing  China  LSIIT 《中国科学D辑(英文版)》2005,(1)

This paper proposes a basic equation of thermal radiation interaction between surface objects on the basis of the principle of heat balance in the interface. The solution of this equation takes account of the contribution of sensible heat flux and latent heat flux more completely, compared with traditional solution for surface cooling and heating processes. By the aid of the experimental data conducted in the Xiaotangshan experimental site, Beijing, both the non-applicability of Kirchoff's law and the measurability of surface emissivity in a non-isothermal system have been highlighted. Two methods called ventilation and time-delay compensations have been proposed to reduce the error induced by change of surface temperatures of non-isothermal objects during the measurement of emissivity. Based on the solution of the basic equation, this paper has analyzed and pointed out the misunderstanding in comprehension and application of Kirchoff's law published in literature.  相似文献   

BULROC反循环潜孔锤     

刘海翔 《国外地质勘探技术》1990,(12):14-15

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UTB—1型超深钻机推进了自动化的发展     

Wehli.  B 刘海翔 《探矿工程译丛》1993,(4):23-27

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从解析摄影测量到数字摄影测量的进展     

Wrob.  BP 周月琴 《测绘译丛》1993,(6):21-27,20

本文概括了从解析摄影测量到数字摄影测量的进展，部分地考虑了所采用的方法，但主要强调数学和物理原理。解析摄影测量的原始信息量测数据是影像坐标，而数字摄影测量原始信息量测数据是数字影像灰度值。从这两种原始数据的本质可以明显地看出解析摄影测量与数字摄影测量的基本区别。对这两种技术，比较了它们的高斯－马尔柯夫估计过程，提出了影像空间和目标空间之间的相互关系；对辐射的考虑比以前起了更重要的作用。数字摄影测量  相似文献   

非开挖行业中的安全问题     

PaulHayward  刘海翔 《岩土钻凿工程》2001,(2):140-142

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A Measurement of Omega from the North American Test Flight of Boomerang     

Melchiorri A  Ade PA  de Bernardis P  Bock JJ  Borrill J  Boscaleri A  Crill BP  De Troia G  Farese P  Ferreira PG  Ganga K  de Gasperis G  Giacometti M  Hristov VV  Jaffe AH  Lange AE  Masi S  Mauskopf PD  Miglio L  Netterfield CB  Pascale E  Piacentini F  Romeo G  Ruhl JE  Vittorio N 《The Astrophysical journal》2000,536(2):L63-L66

We use the angular power spectrum of the cosmic microwave background, measured during the North American test flight of the Boomerang experiment, to constrain the geometry of the universe. Within the class of cold dark matter models, we find that the overall fractional energy density of the universe Omega is constrained to be 0.85相似文献   

Long-Term Landscape Evolution of the Northparkes Region of the Lachlan Fold Belt, Australia: Constraints from Fission Track and Paleomagnetic Data     

O'Sullivan PB  Gibson DL  Kohn BP  Pillans B  Pain CF 《The Journal of geology》2000,108(1):1-16

Apatite fission track thermochronology (AFTT) and paleomagnetic (PM) results have been used to constrain the Late Paleozoic to Cenozoic landscape evolution of the Lachlan Fold Belt (LFB) around the Northparkes copper-gold deposit in east-central New South Wales. The present-day landscape of this region of the LFB is relatively flat with little expression of the underlying rock and has previously been interpreted to indicate long-term stability of the region since the end of LFB orogenesis in the Early Carboniferous. This was presumably borne out by PM analyses from thick weathered horizons within open pits at the mine, which suggested that significant periods of weathering, and hence relative landscape stability, prevailed during the Early to middle Carboniferous and at some time during the Cenozoic. Results from AFTT analyses, however, indicate that the region must have experienced significant episodes of cooling/denudation during the mid-Permian to mid-Triassic and during the early Cenozoic, as well as episodes of heating/burial during the Late Carboniferous to mid-Permian and during the late Mesozoic. When combined, the AFTT and PM results are in fact consistent and indicate that since the late Paleozoic the landscape of the LFB around the Northparkes deposit has evolved through multiple episodes of denudation and deposition as well as periods of relative stability during which the thick weathering horizons formed. Together these results establish a complementary chronological framework that constrains the Late Palaeozoic to Cenozoic landscape evolution of the Northparkes region and highlights the importance of using dual data sets in elucidating the long-term landscape evolution of similar "stable" terranes.  相似文献