Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence     

Canesi L  Scarpato A  Betti M  Ciacci C  Pruzzo C  Gallo G 《Marine environmental research》2002,54(3-5):547-551

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.  相似文献   

海平面     

Woodr.  C 温家洪 《海洋地质译丛》1994,(4):78-80,F003

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Improved form of wind wave frequency spectrum   总被引：5，自引：0，他引：5  

Wen Shengchang 《海洋学报(英文版)》1989,8(4):467-483

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit  相似文献   

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels   总被引：5，自引：0，他引：5  

Scholz S  Kordes C  Hamann J  Gutzeit HO 《Marine environmental research》2004,57(3):235-244

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.  相似文献   

An Experi mental Study on Wave-Induced Dynamic Stresses in Seabed     

S.C.Chang  L.K.Chien  J .G.Lin  Y.F.Chiu 《中国海洋工程》2006,20(1)

1 .IntroductionEvery year ,Taiwanis subjectedtotwo or three typhoonintrusions in average which always causedisasters . Most of the disasters which occur around the coastal zone are due to seabed instability andcoastal structure destruction caused bytyphoo…  相似文献   

The concentration and distribution of dimethyl sulfide in the marine atmospheric boundary layer near the equator     

Li Xingsheng  Li Zhe  F. Parungo  C. Nagamoto 《海洋学报(英文版)》1995,14(3):355-369

Ｔｈｅｃｏｎｃｅｎｔｒａｔｉｏｎａｎｄｄｉｓｔｒｉｂｕｔｉｏｎｏｆｄｉｍｅｔｈｙｌｓｕｌｆｉｄｅｉｎｔｈｅｍａｒｉｎｅａｔｍｏｓｐｈｅｒｉｃｂｏｕｎｄａｒｙｌａｙｅｒｎｅａｒｔｈｅｅｑｕａｔｏｒ￥ＬｉＸｉｎｇｓｈｅｎｇ；ＬｉＺｈｅ；Ｆ．Ｐａｒｕｎｇｏ...  相似文献   

封闭,半封闭虾池的饲养管理技术（摘要）     

Limsu.  C 《台湾海峡》1996,15(A00):126-127

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赤道太平洋的环流     

SP·Hayes  郭洪寿 《海洋预报》1988,(Z1)

一.引 言 赤道太平洋上层海洋环流的主要特征已被建立十多年了。1942年斯维尔德鲁普等人描述了中太平洋的表面流:往西流的北和南赤道流以及往东流的北赤道逆流。随后,南赤道逆流也被用表面动力高度图表示出来(Reia,1961),Cromwell等人(1954)发现了赤道潜流,从而对上述环流的描述进行了主要修正。Hisard和Rual(1970)发现了赤道潜流下面  相似文献   

杭州湾南岸潮滩的~(210)Pb分布及其沉积学意义   总被引：1，自引：0，他引：1  

李炎  G.W.Berger  Tj.C.E.vanWeering 《海洋学研究》1993,(1)

杭州湾南岸潮滩的~(210)Pb垂向分布具有随深度波动的特征。湖滩颗粒物在吸附模拟系统中的~(210)Pb分配系数,主要受颗粒物含量的影响。本文从潮滩沉积~(210)Pb初始比度在低于平衡点一侧波动的机理,以及被沉积间断所分隔的有效封闭段的存在出发,提出选择常态沉积层的高~(210)Pb比度窗口,建立以CIC模式估计潮滩沉积速率的方法。  相似文献   

Marine mammals from northeast Atlantic: relationship between their trophic status as determined by delta13C and delta15N measurements and their trace metal concentrations     

Das K  Beans C  Holsbeek L  Mauger G  Berrow SD  Rogan E  Bouquegneau JM 《Marine environmental research》2003,56(3):349-365

The relationship between trophic position through delta13C and delta15N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the Northeast Atlantic: striped dolphin Stenella coeruleoalba, common dolphin, Delphinus delphis, Atlantic white-sided dolphin Lagenorhynchus acutus, harbour porpoise Phocoena phocoena, white beaked-dolphin Lagenorhynchus albirostris, grey seal Halichoerus grypus stranded on French Channel and Irish coasts. White-beaked dolphins, harbour porpoises, white-sided dolphins, common and striped dolphins display the same relative and decreasing trophic position, as measured by delta15N values, along both the Irish and French channel coasts, reflecting conservative trophic habits between these two places. Hepatic and renal Cd concentrations were significantly correlated to muscle delta13C and delta15N values while Hg, Zn and Cu did not. These results suggest that Cd accumulation is partly linked to the diet while other factors such as age or body condition might explain Hg, Zn or Cu variability in marine mammals. Combined stable isotope and trace metal analyses appear to be useful tools for the study of marine mammal ecology.  相似文献