Terminal‐restriction fragment length polymorphisms (T‐RFLPs) were used to address the question whether there are multiple phylotypes of bacteria within the trophosome of the vestimentiferan tubeworm Ridgeia piscesae in addition to the known endosymbiont. Clone libraries were constructed to aid in the identification of the additional bacterial phylotypes. Individual R. piscesae specimens were collected from Juan de Fuca Ridge, Axial Caldera and Explorer Ridge, Magic Mountain. Clone library analyses revealed only one bacterial phylotype (expected R. piscesae endosymbiont) within the Explorer Ridge trophosomes. However, the Axial Caldera clone library revealed five operational taxonomic units (OTUs). Three of the resulting phylotypes detected (designated RAE) were putative thioautotrophic symbionts within the γ‐Proteobacteria which belonged to the anticipated endosymbiont group (RAE OTU 1), the Maorithyas hadalis symbiont II group (RAE OTU 3), and the Halothiobacillus group (RAE OTU 4). The remaining two phylotypes were most likely opportunistically derived. RAE OTU 5 was an α‐Proteobacterium within the Roseobacter group and RAE OTU 2 was within the Cytophaga–Flavobacterium–Bacteroidetes. T‐RFLP analyses revealed that all 15 trophosomes evaluated contained at least RAE OTUs 1 and 4. Five of the 15 trophosomes contained at least RAE OTUs 1, 3, and 4 and two of the trophosomes contained at least five RAE OTUs. Cluster analysis of the T‐RFLP data revealed three distinct clusters. The number of taxa within the trophosome may be an indication of the general health of the tubeworm. This study strongly suggests that we have discovered and identified more than one bacterial phylotype within the trophosome of vestimentiferan R. piscesae. 相似文献
The relation between the nitrate and phosphate concentrations in the Sea of Okhotsk and the bordering waters of the Pacific Ocean were studied. The surveys were carried out in the autumn, spring, and summer of 2001–2002. For the deepwater part of the sea, the relation [NO?3] = ((14.88 ± 0.07) × [PO3?4] ? 5.46 ± 0.17) was found. The coefficients in the equation given are statistically different from those in the similar equation for the Pacific waters: [NO?3] = (16.05 ± 0.15) × [PO3?4]-(7.23 ± 0.36). In the northern part of the sea; on the shelf; in the slope area; and, especially, in the deep waters of the TINRO Depression, the linear dependence between the phosphate and nitrate concentrations was distorted. This feature was described in terms of nitrate deficiency. The maximum values of this deficiency were found in the near-bottom waters. The principal processes that might cause the nitrate deficiency were considered: the difference in the oxidation rates of the nitrogen and phosphorus organic compounds, the matter transfer between the continent and the sea, the different efficiency of the biogenic burial of nitrogen and phosphorus in the bottom sediments, and the denitrification in the upper layer of the bottom sediments. It was shown that the most probable cause of the nitrate deficiency was the denitrification. The loss of inorganic nitrogen owing to the supply of the waters of the Sea of Okhotsk to the Pacific Ocean was estimated as ~2.5 × 1011 mol N/year. 相似文献
Simultaneous acquisition of water samples, radiance and irradiance measurements were carried out from 40 stations in the Mandovi–Zuari estuaries during February to May 2002. From the samples collected, inherent and apparent optical properties (IOP and AOP) such as absorption coefficient (a), upwelling diffuse attenuation coefficient (ku) and subsurface reflectance (R) were derived. Using these optical properties, radiative transfer at each water column is examined. On the basis of the radiative transfer outcome, band-ratio algorithms are derived for three optically active substances (OAS), viz, chlorophyll-a, suspended sediment and coloured dissolved organic matter (CDOM). The respective algorithms are 670/555, 490/670 and 412/670 nm for chlorophyll-a, suspended sediment and CDOM. These algorithms are applied to Ocean Colour Monitor (OCM), onboard Indian Remote Sensing Satellite (IRS)-Polar Satellite Launch Vehicle (P4), scenes (digital data), to synoptically analyze these OAS. The synoptic analysis of OAS revealed different hydrodynamic characteristics of the estuaries during non-monsoon seasons. 相似文献
Rock-magnetic measurements along with grain size, acid-insoluble residue (AIR), organic carbon (OC), CaCO3 and δ18O of the planktonic foraminifers of the sediments were determined for 15 gravity cores recovered from the western continental margin of India. Magnetic susceptibility (MS) values in the surficial sediments reflect the land-derived input and, in general, are the highest in terrigenous sediment-dominated sections of the cores off Saurashtra–Ratnagiri, followed by the sediments off Indus–Gulf of Kachchh and then Mangalore–Cape Comorin.
The down-core variations in mineral magnetic parameters reveal that the glacial sediments off the Indus are characterized by low MS values/S-ratios associated with high AIR-content, low OC/CaCO3 contents and relatively high δ18O values, while those off SW India are characterized by low MS values/high S-ratio% associated with low AIR content, and relatively high OC, CaCO3 and δ18O values. Conversely, the Early Holocene sediments of all cores are characterized by high MS values/S-ratio% associated with high AIR content, low OC, CaCO3 contents and gradually decreased δ18O values. These results imply that during the Last Glacial Maximum (LGM), the cores off northwestern India received abundant continental supply leading to the predominance of eolian/fluvial sedimentation. In the SW region the influence of hinterland flux is less evident during this period, but convective mixing associated with the NE monsoon resulted in increased productivity. During the early Holocene intense SW monsoon conditions resulted in high precipitation on land, which in turn contributed increased AIR content/MS values in the continental margin sediments. A shallow water core off Kochi further suggests that the intense SW monsoon conditions prevailed until about 5 ka. The late Holocene organic-rich sediments of the SW margin of India were, however, subjected to early diagenesis at different intervals in the cores. Therefore, caution is needed when interpreting regional climatic change from down-core changes in sediment magnetic properties. 相似文献
This paper aims to investigate the basic interaction characteristics of side-by-side moored vessels both numerically and experimentally. A higher-order boundary element method (HOBEM) combined with generalized mode approach is applied to analysis of motion and drift force of side-by-side moored multiple vessels (LNG FPSO, LNGC and shuttle tankers). Model tests were carried out for the same floating bodies investigated in the numerical study in regular and irregular waves. Global and local motion responses and drift forces of three vessels are compared with those of calculations. Discussions is highlighted on applicability of numerical method to prediction of sophisticated multi-body interaction problem of which motion behavior is very important to analysis of mooring dynamics of deep sea floating bodies. 相似文献
We study elevations of the level of a homogeneous ocean caused by a large-scale elliptic anomaly of the field of atmospheric pressure and determine the conditions under which Earth's rotation and the asymmetry of the region of disturbances of the baric field lead to significant deviations of the ocean level from its values given by the law of inverted barometer. Our investigation is based on the numerical analysis of the integral solution of the linear problem. It is shown that the deviations from the law of inverted barometer are especially pronounced in shallow-water regions, at high latitudes, and for strongly asymmetric regions of disturbances of atmospheric pressure. 相似文献
Backscatter reflectivity from multibeam echo-sounders provides a powerful tool to efficiently characterize seafloor substrates. A comprehensive EM300 bathymetric and backscatter survey has been completed of Cook Strait, in central New Zealand. This paper presents a detailed analysis of the realtime corrections applied to the raw EM300 multibeam data and additional corrections required to compute angular variations of the backscatter strength. The corrections, including the local absorption coefficient, the influence of seafloor topography and sound refraction in the water column, are determined for different Cook Strait seafloor substrates. Modifying MB-System software code, we extracted the backscatter signal parameters in order to quantify the raw backscatter strength and apply additional processing. Profiles of backscatter strength versus incidence angle were computed for a variety of sites characterized by flat seafloor and homogeneous substrates, and for which ground-truth data were available. For each homogeneous site, different but characteristic backscatter profiles are observed that can be interpreted in terms of sediment facies. To analyze heterogeneous substrates, we present a statistical technique, based on a 3-dimensional distribution of (incidence angle, backscatter strength) couples that preserves geological information of the substrate components. This analysis, using backscatter data acquired on a submarine volcano, north of New Zealand, clearly differentiates soft sediments and lava flows within a heterogeneous substrate. 相似文献
A coupled wave–tide–surge model has been developed in this study in order to investigate the effect of the interactions among tides, storm surges, and wind waves. The coupled model is based on the synchronous dynamic coupling of a third-generation wave model, WAM cycle 4, and the two-dimensional tide–surge model. The surface stress, which is generated by interactions between wind and wave, is calculated by using the WAM model directly based on an analytical approximation of the results using the quasi-linear theory of wave generation. The changes in bottom friction are created by the interactions between waves and currents and calculated by using simplified bottom boundary layer model. In consequence, the combined wave–current-induced bottom velocity and effective bottom drag coefficient were increased in the shallow waters during the strong storm conditions. 相似文献
Ringnet fishing began in the early 20th century and is practised worldwide, mainly to target nearshore pelagic species. The method was introduced to Kenya’s coastal waters by migrant fishers from Tanzania. However, the impacts of this fishing gear remain poorly assessed. We assessed the spatial distribution of ringnet fishing effort and its possible effects on ecosystem components, such as coral reefs, marine megafauna and marine protected areas, on the south coast of Kenya. We tracked 89 ringnet fishing trips made from December 2015 to January 2016 and used spatial multicriteria analysis to determine hotspots of possible environmental risks. The results showed that habitat type and bathymetric profile influenced the spatial distribution of ringnet fishing effort. Mixed seagrass and coral habitats had the highest concentration of the effort. Most of the habitats in the study area were moderately exposed to the impacts of the ringnet fishery. The study identifies high-risk areas that require spatial measures to minimise possible environmental risks of the gear both to habitats and to endangered sea turtles. 相似文献