Cytochrome P450 differences in normal and imposex-affected female whelk Buccinum undatum from the open North Sea     

Santos MM  ten Hallers-Tjabbes CC  Vieira N  Boon JP  Porte C 《Marine environmental research》2002,54(3-5):661-665

Normal and imposex-affected female Buccinum undatum were sampled from the open North Sea at three locations, one with low, and two with high shipping densities. Cytochrome P450 components and P450 aromatase activity were determined in the microsomal fractions isolated from pooled digestive gland/gonads. Cytochrome P450 aromatase activity was significantly higher (P < 0.05) in normal females collected in the low shipping density area (1,325 +/- 295 fmol/h/mg protein) than levels from imposex animals from a high shipping density area (620 +/- 287 fmol/h/mg protein). A negative correlation was found between aromatase activity and organotin body burden (r = -0.99). Levels of CYP450, cytochrome b5 and NADPH cytochrome c reductase activity did not show differences among groups. This is the first field evidence of depressed aromatase activity in imposex affected females, although additional research under laboratory controlled conditions is required to fully understand the mechanisms underlying the development of imposex in this species.  相似文献   

Longshore drift estimation using fluorescent tracers: New insights from an experiment at Comporta Beach, Portugal   总被引：1，自引：0，他引：1  

Ana Silva  Rui Taborda  Aurora Rodrigues  Joo Duarte  Joo Cascalho 《Marine Geology》2007,240(1-4):137-150

A fluorescent sand-tracer experiment was performed at Comporta Beach (Portugal) with the aim of acquiring longshore sediment transport data on a reflective beach, the optimization of field and laboratory tracer procedures and the improvement of the conceptual model used to support tracer data interpretation.

The field experiment was performed on a mesotidal reflective beach face in low energetic conditions (significant wave height between 0.4 and 0.5 m). Two different colour tracers (orange and blue) were injected at low tide and sampled in the two subsequent low tides using a high resolution 3D grid extending 450 m alongshore and 30 m cross-shore. Marked sand was detected using an automatic digital image processing system developed in the scope of the present experiment.

Results for the two colour tracers show a remarkable coherence, with high recovery rates attesting data validity. Sand tracer displayed a high advection velocity, but with distinct vertical distribution patterns in the two tides: in the first tide there was a clear decrease in tracer advection velocity with depth while in the second tide, the tracer exhibited an almost uniform vertical velocity distribution. This differing behaviour suggests that, in the first tide, the tracer had not reached equilibrium within the transport system, pointing to a considerable time lag between injection and complete mixing. This issue has important implications for the interpretation of tracer data, indicating that short term tracer experiments tend to overestimate transport rates. In this work, therefore, longshore estimates were based on tracer results obtained during the second tide.

The estimated total longshore transport rate at Comporta Beach was 2 × 10^− 3 m³/s, more than four times larger than predicted using standard empirical longshore formulas. This discrepancy, which results from the unusually large active moving layer observed during the experiment, confirms the idea that most common longshore transport equations under-estimate total sediment transport in plunging/surging waves.  相似文献   

Microphytobenthos vertical migratory photoresponse as characterised by light-response curves of surface biomass     

Joo Serdio  Helena Coelho  Snia Vieira  Snia Cruz 《Estuarine, Coastal and Shelf Science》2006,68(3-4):547

The migratory response of intertidal microphytobenthos to changes in irradiance was studied on undisturbed estuarine sediments. Two non-destructive optical techniques were used to trace variations in vivo of surface biomass: PAM fluorometry, for measuring the minimum fluorescence level (F_o); and spectral reflectance analysis, for quantifying the normalized difference vegetation index (NDVI). Following the formation of a dense biofilm at the surface, replicated sediment samples were simultaneously exposed to six different irradiance levels, ranging from 50 to 1500 μmol m⁻² s⁻¹, during a period of 120 min. The migratory photoresponse of the biofilms was characterised by constructing biomass vs. light curves (BLC), relating the accumulation of microalgal biomass after that period (estimated by F_o or NDVI) to the irradiance level incident on the surface. BLCs allow characterising the main features of the migratory photoresponse of intact biofilms. Typical BLC showed a clear biphasic pattern, with an increase in microalgal accumulation under irradiances below 100 μmol m⁻² s⁻¹, maximum values under 100–250 μmol m⁻² s⁻¹, and a gradual decrease of surface biomass under higher irradiances, indicating a strong photophobic downward migratory response. Similar BLC patterns were obtained when measuring F_o or NDVI. The construction of BLCs for biofilms from intertidal sites with distinctive sediment characteristics and diatom taxonomic composition allowed to detected significant differences in the migratory photoresponse. Biofilms from a muddy sediment exhibited considerably larger amplitude in the migratory photoresponse than the biofilms from a sandy mud site, especially under high irradiances. The photophobic migratory response to high light was found to vary among diatom species, particularly in the case of the biofilms from the muddy sediments.  相似文献   

Measuring lysosomal stability as an effective tool for marine coastal environmental monitoring     

Castro M  Santos MM  Monteiro NM  Vieira N 《Marine environmental research》2004,58(2-5):741-745

The use of lysosomal stability in the mussel, Mytilus galloprovincialis, as a potential biomarker of environmental contamination has been evaluated along the Portuguese coast. To this end, the neutral red retention (NRR) time was measured in mussel haemocytes gathered from nine different locations reflecting different degrees of anthropogenic contamination. Mussels collected in the vicinity of industrial and urban areas showed the lowest lysosomal stability. Additionally, no significant seasonal variability (winter-spring/summer) for NRR time was observed. In order to further support the usefulness of this method as an integrated tool for monitoring marine coastal environments, we compared the levels of xenobiotics in mussel tissues with the obtained NRR values. The results highlighted a consistent pattern, with the lowest lysosomal stability intimately correlated with the higher contaminant concentrations. In summary, this integrated approach further demonstrated that the NRR assay can provide useful and objective indications of the real health status of organisms subjected to different stress agents, being a valid option for environmental monitoring.  相似文献   

Modelling linear wave transformation induced by dissipative structures—Random waves     

R. Silva  M.A. Losada  P. Salles 《Ocean Engineering》2006,33(16):2174-2194

The relevant theory is presented and numerical results are compared with the analytical solution for the interaction of non-breaking waves with an array of vertical porous circular cylinders on a horizontal bed. The extension to the cases of unidirectional and multidirectional waves is obtained by means of a transfer function. The influence of the mechanical properties of porous structures and wave irregularity on wave transformation is analysed. Results for unidirectional and multidirectional wave spectra are compared to those obtained for regular waves. The model presented reproduces well the analytical results and provides a tool for analysing several engineering problems.  相似文献   

Influence of initial plastic anisotropy of overconsolidated clays on ground behaviour during tunneling     

J. R. Maranha  A. Vieira 《Acta Geotechnica》2008,3(4):259-271

In order to evaluate the influence of the initial plastic anisotropy in the excavation of a tunnel, a “bubble” bounding surface model for structured soils, formulated by Kavvadas and Belokas (Proc. 10th IACMAG Conf., 2001), was implemented in the explicit finite difference code FLAC. Two different initial stress (K ₀) conditions were considered. The size and shape of the initial bounding surfaces were specified to be consistent with the initial stress field. The distorted and rotated shape of the bounding surface, supported by experimental results, defines the anisotropy of shear strength, which is shown to have a significant influence on the displacements. There is also considerable sensitivity of the soil model to the initial stress field.  相似文献   

Extension of the solution of Kepler's equation to high eccentricities     

Sandro Da Silva Fernandes 《Celestial Mechanics and Dynamical Astronomy》1994,58(3):297-308

The classic Lagrange's expansion of the solutionE(e, M) of Kepler's equation in powers of eccentricity is extended to highly eccentric orbits, 0.6627 ... <e<1. The solutionE(e, M) is developed in powers of (e–e*), wheree* is a fixed value of the eccentricity. The coefficients of the expansion are given in terms of the derivatives of the Bessel functionsJ _n (ne). The expansion is convergent for values of the eccentricity such that |e–e*|<(e*), where the radius of convergence (e*) is a positive real number, which is calculated numerically.  相似文献   

Fluctuation in the zooplankton community in two solar salt ponds,Aveiro, Portugal     

N. Vieira  F. Amat 《International Journal of Salt Lake Research》1995,4(4):327-333

The zooplankton of two salt ponds at Aveiro was studied to evaluate its density and diversity. Samples were collected biweekly from the salt ponds Esmolas and Tanoeiras. Samples were first separated into Holoplankton (Copepoda, nauplii,Acartia, Ostracoda and Anostraca) and Meroplankton (Mollusca, Insecta, annelidan larvae and Ichthyoplankton). The Holoplankton was mainly composed of:Acartia tonsa, Acartia sp.,Eurytemora velox, Artemia sp., and harpacticoids and calanoids. In both salt ponds, species diversity was identical, but total zooplankton density was higher in the Tanoeiras salt pond, probably because its physical and chemical characteristics allowed the development of stable communities.  相似文献   

Holocene record of productivity in the NW Iberian continental shelf     

V. Alves Martins  C. Patinha  E. Ferreira da Silva  J.-M. Jouanneau  O. Weber  F. Rocha   《Journal of Geochemical Exploration》2006,88(1-3):408

A multiproxy approach (textural characteristics, heavy metal concentrations and benthic foraminifera data) was adopted to study the OMEX core KSGX 40, collected at the Galicia Mud Deposit (NW Iberian outer continental shelf) and recording the last ca. 4.8 ka cal BP. Geochemical profiles of Fe, Mn, Zn, Co, Cr, Cu, Ni, Pb, Al and Ca show significant temporal variations. Benthic foraminifera productivity proxy suggests that the recorded muddy events were contemporaneous of a higher C_org flux to the marine benthic environment. The higher flux of organic matter, following a long-term intensification of the upwelling and coinciding with a finer sedimentation, led to depressed levels of oxygen beneath the water-sediments interface and to early diagenetic processes of several redox-sensitive elements.  相似文献   

Study of geothermal water intrusion due to groundwater exploitation in the Puebla Valley aquifer system, Mexico   总被引：1，自引：1，他引：1  

E. Leticia Flores-Márquez  Gabriel Jiménez-Suárez  Raymundo G. Martínez-Serrano  René E. Chávez  Daniel Silva Pérez 《Hydrogeology Journal》2006,14(7):1216-1230

Significant intrusion of geothermal water into fresh groundwater takes place in the Puebla Valley aquifer system, Mexico. The decline in the potentiometric surface due to the overexploitation of the groundwater induces this intrusion. This hydrological system comprises three aquifers located in Plio-Quaternary volcanic sediments and Mesozoic calcareous rocks. The hydraulic balance of the aquifer shows that the annual output exceeds the natural inputs by 12 million m³. Between 1973 and 2002, a drop in the potentiometric surface, with an 80 m cone of depression, was identified in a 5-km-wide area located southwest of the city of Puebla. Chemical analyses performed on water samples since 1990 have shown an increase in total dissolved solids (TDS) of more than 500 mg/L, coinciding with the region showing a cone of depression in the potentiometric surface. A three-dimensional flow and transport model, based on the hydrogeological and geophysical studies, was computed by using the MODFLOW and MT3D software. This model reproduces the evolution of the aquifer system during the last 30 years and predicts for 2010 an additional drawdown in the potentiometric surface of 15 m, and an increase in the geothermal water intrusion.  相似文献