Seasonal variation in lysosomal destabilization in oysters,Crassostrea virginica     

Ringwood AH  Hoguet J  Keppler CJ 《Marine environmental research》2002,54(3-5):793-797

Lysosomal destabilization assays have been used as valuable biomarkers of pollutant exposures in a variety of bivalve and fish species. The responses of oysters, Crassostrea virginica, deployed at and native to various reference and degraded sites were evaluated for lysosomal destabilization during both summer and winter seasons. In both native and deployed oysters, lysosomal destabilization rates tended to be higher during the winter at both reference and polluted sites. There are at least two hypothetical explanations. Greater lysosomal destabilization rates may be related to physiological changes associated with mobilization of nutrient reserves during the winter and gametogenesis. However, lysosomal destabilization in deployed oysters was correlated with tissue metal concentrations. These data also support a second hypothesis that seasonal differences in physico-chemical factors (such as reduced levels of acid volatile sulfides) may increase the bioavailability of metals during the winter so that adverse effects are more pronounced.  相似文献   

Voltammetric estimation of iron(III) thermodynamic stability constants for catecholate siderophores isolated from marine bacteria and cyanobacteria     

Brent L. Lewis  Pamela D. Holt  Steven W. Taylor  Steven W. Wilhelm  Charles G. Trick  Alison Butler  George W. Luther  III 《Marine Chemistry》1995,50(1-4)

Thermodynamic stability constants have been estimated for the complexation of iron(III) with catecholate-type siderophores isolated from the marine bacterium Alteromonas luteoviolacea and from the marine cyanobacterium Synechococcus sp. PCC 7002. Stability constants were determined utilizing the “chelate scale” of Taylor et al. (1994). The scale is based upon a linear relationship between the reduction potentials and the pH-independent thermodynamic stability constants for known iron(III) complexes. Log K values for the alterobactin B ferric iron complex are 43.6 ± 1.5 at pH 8.2 and 37.6 ± 1.2 at pH 6, consistent with a shift from bis-catecholate to monosalicylate/monocatecholate iron coordination with decreasing pH. Synechococcus isolates PCC 7002 Nos. 1 and 3 formed iron(III) complexes with stability constants of approximately 38.1 ± 1.2 and 42.3 ± 1.5, respectively. The binding strengths of the iron(III) complexes examined in this study are quite high, suggesting that catecholate siderophores may play a role in the solubilization and biological uptake of iron in the marine environment.  相似文献   

Spatially explicit dispersal dynamics and equilibrium population sizes in marine harvest refuges     

Acosta  Charles A. 《ICES Journal of Marine Science》2002,59(3):458-468

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用纤维素乙酸平板电泳测定中国卤虫的同功酶变异(英文)     

张雷  CharlesE.king 《中国海洋大学学报(自然科学版)》1991,(4)

本文报导了应用纤维素乙酸平板电泳方法,测定采于中国山东省东方红盐场卤虫(Artemia)的同功酶。经测定,8种同功酶其中有6种是多态的,即:苹果酸脱氢酶(MDH)、异柠檬酸酶(IDH)、谷氨酸草醋酸转移酶(GOT)、葡糖-6-磷酸盐脱氢酶(D6PHD)、6-磷酸萄糖脱氢酶(6PGDH)和磷酸葡糖异构酶(PGI)。本文所提供的研究方法,不仅适用于基础研究,而且在水产养殖业上有助于对卤虫的筛选和确证。  相似文献   

Code of Practice For Ocean Mining: An International Effort to Develop a Code For Environmental Management of Marine Mining     

Anthony T. Jones  Charles L. Morgan 《Marine Georesources & Geotechnology》2003,21(2):105-114

A code of practice for ocean mining has been developed by the International Marine Minerals Society. The code provides general guiding Principles for development of marine mineral resources and delineates Operating Guidelines for application, as appropriate, at specific mining sites. In total 11 operating guidelines are provided to serve as benchmarks for industry and targets for regulatory agencies and other stakeholders. They include the following commitments: •To sustainable development, •To environmentally responsible corporate ethics, •To the development of effective community partnership, •To implementation of environmental risk management, •To the integration of environmental management into all phases of development, •To the establishment of corporate environmental performance targets, •To timely environmental improvements and upgrading, •To responsible rehabilitation of operations sites and decommissioning of facilities, and •To accurate and timely, reporting and documentation, archiving, and performance review.  相似文献   

Indirect economic indicators in bio-economic fishery models: agricultural price indicators and fish stocks in Lake Victoria   总被引：2，自引：0，他引：2  

Simonit  Silvio; Perrings  Charles 《ICES Journal of Marine Science》2005,62(3):483-492

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Influence of relative sea level changes on the construction of the Mississippi Fan     

Arnold H. Bouma  James M. Coleman  Charles E. Stelting  Barry Kohl 《Geo-Marine Letters》1989,9(3):161-170

A conceptual sea-level-driven depositional model for individual fanlobes (channel-overbank systems) of the Mississippi Fan does not permit direct application of the sequence stratigraphic principles of Vail and colleagues. Deep Sea Drilling Project Leg 96 results suggest that, during initial relative lowering of sea level, the canyon and upper fan channel were formed; excavated fine-grained slope sediments may have formed a debris flow deposit base for the fanlobe. Continued lowering produced constructional channel-levee-overbank deposits. Rising relative sea level inhibited input of coarse clastics, and channel depressions filled with muds. A blanket of (hemi)pelagics represents relative high sea level stand.  相似文献   

Simplified limit solutions for the capacity of suction anchors under undrained conditions   总被引：1，自引：0，他引：1  

Charles Aubeny  J. Donald Murff   《Ocean Engineering》2005,32(7):864-877

Upper bound plastic limit analyses (PLA) can provide a useful framework for estimating the load capacity of suction caisson anchors in purely cohesive soils. Since arbitrary assumptions regarding the soil stress state are not required in the PLA formulation, it may be used with greater consistency compared to other simplified approaches such as limit equilibrium methods. While PLA methods do not attempt to include all of the complexities of anchor behavior, they can provide a relatively simple framework for visualizing anchor kinematics leading to an understanding of the relative importance of various parameters on suction anchor load capacity. The most rigorous PLA formulations involve postulating a three-dimensional anchor-soil failure mechanism and deriving expressions for internal energy dissipation throughout the mechanism. This approach can involve extensive numerical integrations and a relatively complex scheme for optimizing the failure mechanism to obtain a least upper bound collapse load. Considerable simplification is possible if the problem is formulated in terms of ultimate unit resistances (lateral, axial, and their interaction) that can be exerted by the soil on the caisson. In this case, the caisson failure mechanism can be characterized in terms of one or two optimization variables. Simple expressions for the ultimate unit resistances acting on the caisson can be obtained from several sources including rigorous PLA solutions, finite element techniques, or experimental measurements. General expressions are possible by limiting consideration to common, idealized strength profiles such as uniform or constant gradient. Such simplified formulations are particularly valuable for providing an analysis tool accessible to practicing engineers. Suction caisson anchors can be subjected to a variety of load orientations including nearly vertical uplift forces imposed by the vertical tendons of tension leg platforms, horizontal loads imposed by catenary mooring systems, and inclined loads imposed by taut moorings. Recently, PLA methods have been applied to the analysis of suction caissons subjected to this range of loading conditions. This paper reviews the formulation of these analyses and summarizes the most significant findings.  相似文献   

Automated Kerogen Classification in Microscope Images of Dispersed Kerogen Preparation     

L. I. Kuncheva  J. J. Charles  N. Miles  A. Collins  B. Wells  I. S. Lim 《Mathematical Geosciences》2008,40(6):639-652

We develop the classification part of a system that analyses transmitted light microscope images of dispersed kerogen preparation. The system automatically extracts kerogen pieces from the image and labels each piece as either inertinite or vitrinite. The image pre-processing analysis consists of background removal, identification of kerogen material, object segmentation, object extraction (individual images of pieces of kerogen) and feature calculation for each object. An expert palynologist was asked to label the objects into categories inertinite and vitrinite, which provided the ground truth for the classification experiment. Ten state-of-the-art classifiers and classifier ensembles were compared: Naïve Bayes, decision tree, nearest neighbour, the logistic classifier, multilayered perceptron (MLP), support vector machines (SVM), AdaBoost, Bagging, LogitBoost and Random Forest. The logistic classifier was singled out as the most accurate classifier, with an accuracy greater than 90. Using a 10 times 10-fold cross-validation provided within the Weka software, we found that the logistic classifier was significantly better than five classifiers (p<0.05) and indistinguishable from the other four classifiers. The initial set of 32 features was subsequently reduced to 6 features without compromising the classification accuracy. A further evaluation of the system alerted us to the possible sensitivity of the classification to the ground truth that might vary from one human expert to another. The analysis also revealed that the logistic classifier made most of the correct classifications with a high certainty.  相似文献   

Bayesian estimation of seismic hazard for two sites in Switzerland     

Erik Rüttener  Juan José Egozcue  Dieter Mayer-Rosa  Stephan Mueller 《Natural Hazards》1996,14(2-3):165-178

Seismic hazard analysis is based on data and models, which both are imprecise and uncertain. Especially the interpretation of historical information into earthquake parameters, e.g. earthquake size and location, yields ambiguous and imprecise data. Models based on probability distributions have been developed in order to quantify and represent these uncertainties. Nevertheless, the majority of the procedures applied in seismic hazard assessment do not take into account these uncertainties, nor do they show the variance of the results. Therefore, a procedure based on Bayesian statistics was developed to estimate return periods for different ground motion intensities (MSK scale).Bayesian techniques provide a mathematical model to estimate the distribution of random variables in presence of uncertainties. The developed method estimates the probability distribution of the number of occurrences in a Poisson process described by the parameter . The input data are the historical occurrences of intensities for a particular site, represented by a discrete probability distribution for each earthquake. The calculation of these historical occurrences requires a careful preparation of all input parameters, i.e. a modelling of their uncertainties. The obtained results show that the variance of the recurrence rate is smaller in regions with higher seismic activity than in less active regions. It can also be demonstrated that long return periods cannot be estimated with confidence, because the time period of observation is too short. This indicates that the long return periods obtained by seismic source methods only reflects the delineated seismic sources and the chosen earthquake size distribution law.  相似文献