Effect of benzo[a]pyrene on detoxification and the activity of antioxidant enzymes of marine microalgae     

Chen Shen  Jingjing Miao  Yun Li  Luqing Pan 《中国海洋大学学报(英文版)》2016,15(2):303-310

The objective of this study was to examine the effect of benzo[a]pyrene(Ba P) on the detoxification and antioxidant systems of two microalgae,Isochrysis zhanjiangensis and Platymonas subcordiformis.In our study,these two algae were exposed to Ba P for 4 days at three different concentrations including 0.5 μg~(L-1)(low),3 μg~(L-1)(mid) and 18 μg~(L-1)(high).The activity of detoxification enzymes,ethoxyresorufin O-deethylase(EROD) and glutathione S-transferase(GST) increased in P.subcordiformis in all Ba P-treated groups.In I.zhanjiangensis,the activity of these two enzymes increased at the beginning of exposure,and then decreased in the groups treated with mid-and high Ba P.The activity of antioxidant enzyme superoxide dismutase(SOD) increased in I.zhanjiangensis in all Ba P-treated groups,and then decreased in high Ba P-treated group,while no significant change was observed in P.subcordiformis.The activity of antioxidant enzyme catalase(CAT) increased in I.zhanjiangensis and P.subcordiformis in all Ba Ptreated groups.The content of malondialdehyde(MDA) in Isochrysis zhanjiangensis increased first,and then decreased in high Ba P-treated group,while no change occurred in P.subcordiformis.These results demonstrated that Ba P significantly influenced the activity of detoxifying and antioxidant enzymes in microalgae.The metabolic related enzymes(EROD,GST and CAT) may serve as sensitive biomarkers of measuring the contamination level of Ba P in marine water.  相似文献   

Tide and current observations in the central Chukchi Sea during the summer of 2012     

Huiwu Wang  Na Liu  Hongxia Chen 《中国海洋大学学报(英文版)》2016,15(2):201-208

Current data from a moored Acoustic Doppler Current Profiler(ADCP) deployed at 69?30.155′N,169?00.654′W in the central Chukchi Sea during 2012 summertime is analyzed in the present paper.Characteristics of tidal and residual currents are ob-tained with Cosine-Lanczos filter and cross-spectral analyses.The main achievements are as follows:1) Along with the local inertial frequency of 12.8 h,two other peaks at ~12-h and ~10-d dominate the time series of raw velocity;2) The M_2 dominates the 6 resolved tide constituents with significant amplitude variations over depth and the ratios of current speed of this constituent to that of the total tidal current are 54% and 47% for u and v components,respectively.All the resolved tidal constituents rotate clockwise at depth with the exception of MM and O1.The constituents of M_2 and S_2 with the largest major semi-axes are similar in eccentricity and orientation at deeper levels;3) The maximum of residual currents varies in a range of 20–30 cms~(-1) over depth and the current with lower velocities flow more true north with smaller magnitudes compared to the current in surface layer.The ~10 d fluctuation of residual current is found throughout the water column and attributed to the response of current to the local wind forcing,with an approximate 1.4 d lag-time at the surface level and occurring several hours later in the lower layer;4) Mean residual currents flow toward the north with the magnitudes smaller than 7 cms~(-1) in a general agreement with previous studies,which suggests a relatively weaker but stable northward flow indeed exists in the central Chukchi Sea.  相似文献   

An Investigation of Digestion Methods for Trace Elements in Bauxite and Their Determination in Ten Bauxite Reference Materials Using Inductively Coupled Plasma‐Mass Spectrometry          下载免费PDF全文

Wen Zhang  Liang Qi  Zhaochu Hu  Cunjiang Zheng  Yongsheng Liu  Haihong Chen  Shan Gao  Shenghong Hu 《Geostandards and Geoanalytical Research》2016,40(2):195-216

Trace elements from samples of bauxite deposits can provide useful information relevant to the exploration of the ore‐forming process. Sample digestion is a fundamental and critical stage in the process of geochemical analysis, which enables the acquisition of accurate trace element data by ICP‐MS. However, the conventional bomb digestion method with HF/HNO₃ results in a significant loss of rare earth elements (REEs) due to the formation of insoluble AlF₃ precipitates during the digestion of bauxite samples. In this study, the digestion capability of the following methods was investigated: (a) ‘Mg‐addition’ bomb digestion, (b) NH₄HF₂ open vessel digestion and (c) NH₄F open vessel digestion. ‘Mg‐addition’ bomb digestion can effectively suppress the formation of AlF₃ and simultaneously ensure the complete decomposition of resistant minerals in bauxite samples. The addition of MgO to the bauxite samples resulted in (Mg + Ca)/Al ratios ≥ 1. However, adding a large amount of MgO leads to significant blank contamination for some transition elements (V, Cr, Ni and Zn). The NH₄HF₂ or NH₄F open vessel digestion methods can also completely digest resistant minerals in bauxite samples in a short period of time (5 hr). Unlike conventional bomb digestion with HF/HNO₃, the white precipitates and the semi‐transparent gels present in the NH₄HF₂ and NH₄F digestion methods could be efficiently dissolved by evaporation with HClO₄. Based on these three optimised digestion methods, thirty‐seven trace elements including REEs in ten bauxite reference materials (RMs) were determined by ICP‐MS. The data obtained showed excellent inter‐method reproducibility (agreement within 5% for REEs). The relative standard deviation (% RSD) for most elements was < 6%. The concentrations of trace elements in the ten bauxite RMs showed agreement with the limited certified (Li, V, Cr, Cu, Zn, Ga, Sr, Zr and Pb) and information values (Co, Ba, Ce and Hf) available. New trace element data for the ten RMs are provided, some of which for the first time.  相似文献   

Degradation of dimethyl sulfoxide through fluidized-bed Fenton process: kinetic analysis     

T.-C. Chen  E. M. Matira  M.-C. Lu  M. L. P. Dalida 《International Journal of Environmental Science and Technology》2016,13(4):1017-1028

In this study, fluidized-bed Fenton process (FBF) was used to degrade dimethyl sulfoxide (DMSO), one of the most widely used solvents. Oxidation by Fenton’s reagent, Fe⁺² and H₂O₂, is one of the cheapest advanced oxidation processes due to the high availability of the reagents. FBF is a modified approach that reduces the large amount of iron oxide sludge formed in conventional Fenton process. The optimal treatment efficiencies by FBF with 2 h of reaction were 95.22 % of DMSO degradation and 34.38 % of COD removal at the conditions of 5 mM DMSO, 68.97 g/L SiO₂ carrier, pH_initial 3.0, 5 mM Fe²⁺, and 32.5 mM H₂O₂. The kinetic study was also done to investigate the two stages involved in the oxidation. The first stage fitted the zero reaction order with overall initial rate’s apparent rate constant, k ₁, of ?0.099. The second stage fitted the first order of DMSO degradation, with rate constant, k ₂, of ?0.0005.  相似文献   

Effects of key components of <Emphasis Type="Italic">S. triqueter</Emphasis> root exudates on fractions and bioavailability of pyrene–lead co-contaminated soils     

Y. Hou  X. Liu  X. Zhang  X. Chen  K. Tao 《International Journal of Environmental Science and Technology》2016,13(3):887-896

The key components of S. triqueter root exudates involved 4-oxo-pentanoic acid, succinic acid, glutaric acid, phthalate acid, citric acid, vanillic acid, myristic acid, pentadecanoic acid, decanoic acid, 14-methyl-pentadecanoic acid, hexadecanoic acid, octadecanoic acid and oleic acid, and the content of the water-soluble organic acids (citric acid, succinic acid and glutaric acid) significantly increased in pyrene and lead co-contaminated rhizosphere soil. These three water-soluble organic acids including citric acid, succinic acid and glutaric acid were detected as the specific root exudates of S. triqueter under stress of pollutants for pyrene and lead, so they were chosen as the research objects, and they were added into the bioremediation systems of pyrene and lead co-contaminated wetland soils. Compared with the control, the treatments added the three organic acids always improved the quantity of the bioavailable fraction of pyrene and lead in wetland soils and greatly influenced other chemical states of pyrene and lead fractions in the test concentration range. Under the 50 g kg^?1 of organic acids concentration, the amount of the bioavailable fraction of pyrene and lead increased 41.0 and 872.7 % by citric acid, respectively. The enhancement of bioavailability of pyrene and lead in the wetland soil by adding organic acids generally decreased in the following order: citric acid > succinic acid > glutaric acid. Enhancing effects of organic acids on the bioavailability improvement of pyrene and lead is remarkable.  相似文献   

区域地球化学特征及其主控因素——对内蒙古珠斯楞地区找矿的意义   总被引：1，自引：1，他引：0  

王嘉伟  李小强  陈清石  牛广智 《地质通报》2016,35(9):1544-1549

在1∶5万水系沉积物测量的基础上,对内蒙古额济纳旗珠斯愣地区的地球化学特征及其主控因素进行初步分析,并对元素分布特征、元素相关性分析、单元素异常与构造之间关系、综合异常与构造之间关系进行了探讨。研究发现,Cu、Au、Sb为区内具一定潜力的找矿元素,与呼伦西白-珠斯楞海尔罕反"S"形构造带南端内、外旋面的转换的珠斯楞海尔罕一带形成一个与断裂构造吻合度较高的、半环性的成矿成晕中心。因而推断,区内具有形成与中酸性侵入岩有关的热液铜多金属矿的可能,其中珠斯楞海尔罕铜金矿和道布青乌苏南北金锑矿2个找矿靶区严格受呼伦西白-珠斯楞海尔罕反"S"形构造的控制。  相似文献   

Adsorption of aqueous Pb(II), Cu(II), Zn(II) ions by amorphous tin(VI) hydrogen phosphate: an excellent inorganic adsorbent     

C. Zhu  X. Dong  Z. Chen  R. Naidu 《International Journal of Environmental Science and Technology》2016,13(5):1257-1268

Amorphous tin(VI) hydrogen phosphate (ATHP) was synthesized using the liquid phase precipitation method and served as an adsorbent to remove Pb(II), Cu(II), and Zn(II) from aqueous solutions. The ATHP was characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and nitrogen adsorption–desorption techniques. Adsorption properties were evaluated as a function of pH, reaction time, concentration of reactants, and salinity. Their equilibrium adsorption data were modeled using Freundlich, Langmuir, and Dubinin–Kaganer–Radushkevich isotherms, respectively. The results revealed that adsorption equilibrium reached within 180 min. ATHP indicated good adsorption even below the pH_ZPC, and best adsorption at pH 5 for Pb(II) and Cu(II) and at pH 5.5 for Zn(II) was observed. Equilibrium data fitted better to the Langmuir model for Pb(II) and Cu(II) and fitted better to the Freundlich model for Zn(II). The saturated adsorption capacities deduced from the Langmuir model were 2.425, 1.801, and 0.600 mmol/g for Cu(II), Pb(II), and Zn(II), respectively, indicating an adsorption affinity order of Cu > Pb > Zn. There is a negative correlation between the concentration of NaCl and adsorption capacity of ATHP, yet ATHP still exhibited excellent adsorption having an adsorption capacity of 19.35, 15.16, 6.425 mg/g when the concentration of NaCl was 0.6 mol/L. The free energy (E) was 12.33, 10.70, and 14.74 kJ/mol for Pb(II), Cu(II), and Zn(II), respectively. An adsorption mechanism based on ion exchange between heavy metal ions and H⁺ in the ATHP is proposed. Furthermore, the used ATHP was regenerated by HCl solution and the adsorbent was used repeatedly.  相似文献   

Autonomic visiting in digital 3D scenes     

Song Chen  Liang Cheng 《Earth Science Informatics》2016,9(3):383-400

Human-machine interactive visiting and fixed-route visiting are currently the main roaming modes in digital three-dimensional (3D) scenes. However, in general, when a person visits an attraction area, s/he does not follow a fixed path, but instead wander about according to his/her interests. Here, we propose a new roaming mode, called autonomic visiting. That is, in a digital 3D scene, a user selects several interest spots, then a route connecting these spots can be automatically determined and 3D scene can be seen along this route. This study presents a technical approach that enables the realization of autonomic visiting in 3D scenes. Firstly, Delaunay triangular meshes for the terrain in 3D scene are established. Secondly, a plane-growth algorithm and a line-connection algorithm are introduced to automatically mend the broken parts of these triangular meshes. Thirdly, the triangular meshes are then merged and differently weighted according to different layers. Finally, a progress-zone transmission algorithm is presented to optimal the shortest route, which is derived from A-Star (A*) algorithm. Digital 3D campus of Nanjing University, China, is taken as the experimental materials. The experimental results prove the effect of the proposed approach.  相似文献   

Sand eddies induced by cyclic tilt of a retaining wall     

Chien-Hsun Chen  Dimitrios Kolymbas 《Acta Geotechnica》2016,11(2):269-280

The cyclic tilt of a retaining wall induces a peculiar motion in the backfill (sand), which exhibits closed trajectories (eddies). In this paper, the motion of the backfill has been optically traced and analyzed by means of particle image velocimetry, also known as digital image correlation. The results are of importance for cyclically loaded structures (e.g, piles for off-shore structures) and can also serve to test numerical simulations of large deformation.  相似文献   

Immobilization of copper under an acid leach of colloidal pyrite waste rocks by a fixed-bed column     

Yan Yang  Tianhu Chen  Ping Li  Chengsong Qing  Qiaoqin Xie  Xinmin Zhan 《Environmental Earth Sciences》2016,75(3):205

Colloidal pyrite waste rocks (CPWR) which are mainly composed of colloidal pyrite and siderite widely deposit in sulfide mines in the middle and lower reaches of the Yangtze River belt, China, especially in Tongling City, Anhui Province, China. In this paper, a fixed-bed column was used to investigate the weathering and oxidation of CPWR and its role in immobilizing low-concentration Cu (10 mg L^?1) under weakly acidic leach (pH = 5.0). The experimental results indicated that the breakthrough capacity was around 14.0 mg Cu g^?1 CPWR when Cu²⁺ breakthrough concentration was 0.5 mg L^?1. Sequential extraction of Cu and dithionite–citrate–bicarbonate extraction of free iron in the used CPWR after the column breakthrough indicated that Cu removal by CPWR consisted of the following processes: oxidation of pyrite and dissolution of siderite in CPWR, ferrous oxidation, and adsorption of Cu on ferric (hydr)oxides. This study shows the potential application of CPWR as an effective sorbent for the removal of low-concentration Cu from acid mine drainage.  相似文献