分光光度法测定海洋河弧菌510脂多糖     

张运涛  顾谦群  方玉春  吕元萌  管华诗 《中国海洋大学学报(自然科学版)》2003,33(3):355-360

本文研究了直接测定海洋河弧菌 (vibrio fluvailis) 5 10脂多糖的分光光度法。利用 1,9-二甲基亚甲蓝 (DMB) ,在适当条件下同脂多糖发生染色反应 ,在 5 5 0 nm波长下 ,反应体系的吸光值同脂多糖含量呈线性相关。相关系数为 0 .9993,最低检出限为 10 μg/m L,相对标准偏差为 1.6 2 ,平均回收率为 (10 5± 2 .18) %。  相似文献   

A combined numerical tidal model for the Hangzhou Bay and Qiantang River   总被引：2，自引：0，他引：2  

Cao Deming  Fang Guohong 《海洋学报(英文版)》1989,8(4):485-496

-In order to avoid prescribing open boundary condition on the upstream side of the Hangzhou Bay, in numerical simulation of the tides and residual currents of the Bay, a 1-D model for the Qiantang River is connected to the 2-D model for the Hangzhou Bay. The harmonic constants of diurnal constituent [ (K1 O1)/2],semidiurnal constituent (M2) and shallow water constituent (M4) are obtained. The results produced by the combined model are in better agreement with the observed ones than those produced solely by the original 2-D model. The combined model gives much more reliable results for tide-induced residual water level and current.  相似文献   

鲢鱼(Hypophthalmichthys molitrix)摄食强度和节律的实验研究     

王芳  董双林  李德尚  邴旭文 《中国海洋大学学报(自然科学版)》1993,(2)

报道用室内实验方法对鲢鱼摄食强度和摄食节律的研究结果,探讨了影响它们的因子。提出鲢鱼在不同水温、不同花粉密度及不同鱼规格下的摄食量的统计分析。指出:食粒密度和水温与摄食量正相关,而鱼规格与摄食量负相关;食粒密度对摄食量影响最大(P<0.01),其次为水温;水温和食粒密度在影响摄食量的过程中可能有一定的交互作用。鲢鱼摄食强度在室内条件下也具明显的昼夜节律。在食粒规格较小、密度较低且溶氧充足条件下,鲢鱼夜晚非但不停食且出现摄食率次高峰。在本实验条件下,鲢鱼的日摄食节律与水温和光照无关,可能受饵料可得性和自身的生物钟节律等制约。  相似文献   

On the long-term joint distribution of characteristic wave height and period and its application     

Fang Zhongsheng  Dai Shunsun  Jin Chengyi 《海洋学报(英文版)》1989,8(3):315-325

By analysing the scatter diagrams of characteristic the wave height H and the period T on the basis of instrumental data from various ocean wave stations, we found that the conditional expectation and standard deviation of wave period for a given wave height can be better predicted by using the equations of normal linear regression rather than by those based on the log- normal law. The latter was implied in Ochi' s bivariate log-normal model(Ochi. 1978) for the long-term joint distribution of H and T. With the expectation and standard deviation predicted by the normal linear regression equations and applying proper types of distribution, we have obtained the conditional distribution of T for given H. Then combining this conditional P(T / H) with long-term marginal distribution of the wave height P(H) we establish a new parameterized model for the long-term joint distribution P(H,T). As an example of the application of the new model we give a method for estimating wave period associated with an extreme w  相似文献   

潮汐分析和预报的准调和分潮方法 Ⅱ.短期观测的分析     

方国洪 《海洋科学集刊》1976,(11):33-56

目前,调和方法仍然是潮汐的分析和预报中的一种主要的基本方法,其关键在于求得尽可能准确的调和常数。在湖汐分析中,经常遇到的是对于中、长期(半个月以上)记录的处理,对于这样的资料,已有许多良好的分析方法可以采用。但是,有时我们也会遇到需要处理的短期观测资料,特别对于潮流,由于对它进行观测比水位观测要困难,常常不能获得较长期的记录,而需要从短期记录中分析出调和常数来。处理短期记录的方法也有不少,其中较为常用的是英国海军部的几种方法。这些方法只能处理一次或二次周日观测记录,当观测天数较多时,只能分组进行计算得到几组调和常数,然后再求平均值由于各组的天文条件的差別,调和常数的准确度常常有相当大的差异,而计算平均值时却没有加权,这就影响了结果的可靠性。本文建议的方法将可以直接分析任意多次周日观测记录,因而简化了计算并能得到更准确的结果。  相似文献   

文昌鱼哈氏窝匀浆使蟾蜍足细胞释精的超微结构研究     

方永强  王龙 《台湾海峡》1991,(1)

本文报道了文昌鱼哈氏窝匀浆刺激蟾蜍足细胞释放精子过程所发生的超微结构变化,进一步证实这种匀浆含有类似脊椎动物促黄体生成素活性物质。  相似文献   

Studies on ultrastructure of oocyte in process of maturing in pen shell     

Fang Yongqiang  Qi Xiang 《海洋学报(英文版)》1988,7(3):459-472

The present paper deals with the ultrastructural changes of oocyte in the process of maturing in pen shell Atrinapectinata Linne. When it develops from the antecedent growth period to the accelerating growth period the annulate lamellae emerge, the RER better develop, the yolk granules with single and double layer membrane and are visible. In the well-developed mitochondria there is yolk stored. The Golgi vacuoles are brewing yolk granules. At the mature period the annulate lamellae and the Golgi bodies disappear, and there are only a few RER and mitochondria with poor cristae in the cytoplasm. All these might be well used for judging the maturity of the ootids in the process of the artificial propagation.  相似文献   

Study of zooplankton ecology in Zhejiang coastal upwelling system-Zooplankton biomass and abundance of major groups   总被引：1，自引：0，他引：1  

He Dehu  Yang Guanming  Fang Shaojin  Shen Weilin  Liu Hongbin  Gao Aigen  Huang Shuxin 《海洋学报(英文版)》1988,7(4):607-620

The studied area is a shallow water area that belongs to the East China Sea continent shelf. The distribution of zooplankton biomass is higher inshore than offshore. The maximum abundance is in the inshore edge of the centre of upwelling, which is the superposed area of the salinity front and temperature front, due to the fact that the mixture of three different waters has brought about a concentration of nutrients. The herbivorous Euphausia, Copepoda and Tunicata are the major groups of the abundant area. Tunicata possess the possibility to compete against other kinds of herbivorous zooplankton. This means that there is negative correlation between the distribution of Tunicata and that of Copepoda and Euphausia. The positions of maximum areas of zooplankton biomass, phytoplankton individual and the concentration of phosphates and chlorophyll-a overlap one another. In the centre of upwelling, zooplankton can not adapt itself to the environment of lower temperature and less oxygen even with rich nutrient  相似文献   

海带的一个自然突变型   总被引：1，自引：0，他引：1  

方宗熙  欧毓麟  崔竞进 《海洋学报》1982,4(2):201-208

在多细胞藻的遗传学研究中,被发现的突变型很少[1].绿藻中有一些突变型,其中较多的是某种石莼(Ulva mutabilis)[2].三浦昭雄等在紫菜中发现突变型.在褐藻中未见有突变型的报道.这里所报道的是海带(Laminaria japonica)的一个突变型.  相似文献   

海坛岛海岸风沙特征及其发育   总被引：7，自引：0，他引：7  

陈方 《海洋科学》1994,18(6):46-50

论述了海坛岛海岸风沙堆积物的沉积特征，对风沙地貌的基本类型进行了划分，分析其发育的基本条件和触发因素，研究其发育历史。  相似文献