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941.
942.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
943.
R. S. Bortkovskii B. N. Egorov V. M. Kattsov T. V. Pavlova 《Izvestiya Atmospheric and Oceanic Physics》2007,43(3):378-383
Annual mean fluxes of CO2 and oxygen across the sea surface are estimated with the use of numerical modeling for several regions located in the Gulf Stream and Kuroshio zones. The present-day climatic conditions and the climatic conditions expected in the middle and at the end of the 21st century are considered. Specific features of gas exchange under a strong wind that are associated with gas exchange by bubbles and with changes in the air-water difference of the gas concentrations were taken into account in the calculations. The estimates obtained differ substantially from the results based on the traditional approach, which disregards the above features. A considerable increase in the absorption of CO2 by the ocean, which is mainly caused by the continuing increase in the CO2 concentration in the air during its small changes in the ocean, is expected in the 21st century. At the same time, no trends are revealed in the annual mean fluxes of oxygen across the ocean surface. The conclusion is made that, in calculations of CO2 absorption by the world ocean, it is necessary to take into account both specific features of gas transfer under a strong wind and an increase in the atmospheric concentration of CO2. 相似文献
944.
The results of continuous seismic profiling thermodynamics performed in the northern part of the Japan Basin in the region of the Tarasov Rise and the data of a micropaleontological examination of the diatom remains encountered in the sediment samples from the rise and continental slope are presented. In the area studied, the topography of the acoustic basement features a vast rise (plateau) buried under the sedimentary cover outlined by the depth contour 5.8 s. The plateau has a relatively smooth top surface crossed by a series of rises of the acoustic basement. The two largest rises are represented by the ridges of the Tarasov Rise. The plateau is separated from the continental slope by a depression in the acoustic basement with a depth up to 6.8 s. From the end of the Middle Miocene up to the beginning of the Paleocene, the region of the plateau represented an area of active volcanism; it coincided in time with the stage of subsidence of the floor of the acoustic basement depression. At the end of the Late Miocene, the ridges of the Tarasov Rise started to sink. In the Pliocene, this process accelerated, and, at the beginning of the Pleistocene, it stopped. In the Middle Miocene-Early Pleistocene time, the portion of the continental slope adjacent to the plateau remained stable and suffered no significant vertical movements. 相似文献
945.
O. O. Trusenkova S. V. Stanichnyi Yu. B. Ratner 《Izvestiya Atmospheric and Oceanic Physics》2007,43(5):634-648
The expansion of wind fields observed at fixed times (four times daily) in complex empirical orthogonal functions is performed for the Japan Sea area (34°–53° N, 127°–143° E). The wind fields are taken from the 1998–2004 NCEP/NCAR Reanalysis data with better spatial resolution (1° × 1°) than the standard product, which are publicly available on the Internet. Major modes of wind variability in the Japan Sea area are identified. The modes determine a general direction of air-mass transport throughout a year, zonal and meridional modulation, and a cyclonic and an anticyclonic eddy component. Objective classification of wind fields with respect to the prevailing flow direction is performed, and wind stress and wind-curl patterns are obtained for major events in the cold and warm periods of the year. The pattern obtained can be used in hydrodynamic numerical models of the general circulation of the Japan Sea. 相似文献
946.
Tropospheric lapse rate and its relation to surface temperature from reanalysis data 总被引:2,自引:0,他引:2
Estimates of the tropospheric lapse rate γ and analysis of its relation to the surface temperature T s in the annual cycle and interannual variability have been made using the global monthly mean data of the NCEP/NCAR reanalysis (1948–2001). The tropospheric lapse rate γ is about 6.1 K/km in the Northern Hemisphere (NH) as a whole and over the ocean and about 6.2 K/km over the continents. The value of γ decreases from 6.5 K/km at low latitudes to 4.5 K/km at polar latitudes. The values of dγ/dT s, the parameter of sensitivity of γ to the variation of T s for the NH in the interannual variability, are found to be about 0.04 km?1 (0.041 km?1 for the NH as a whole, 0.042 km?1 over the ocean, and 0.038 km?1 over the continents). This corresponds to an increase in γ of approximately 0.7% when the surface temperature of the NH is increased by 1 K. Estimates of dγ/dT s vary from about 0.05 km?1 in the subtropics to 0.10 km?1 at polar latitudes. When dγ/dT s is positive, the surface and tropospheric warming means a temperature decrease above a certain critical level H cr. The height of the level H cr with constant temperature, which is defined by the inverse value (dγ/dT s)?1, is about 25 km for the NH as a whole, i.e., above the tropopause. In the subtropics, H cr is about 20 km. At polar latitudes, H cr decreases to about 10 km. Positive values of dγ/dT s characterize a positive climatic feedback through the lapse rate and indicate a general decrease in the static stability of the troposphere during global warming. Along with a general tendency of γ to increase with rising T s, there are regional regimes with the opposite tendency, mainly over the ocean. The negative correlation of γ with T s is found over the oceanic tropics and midlatitudes, in particular, over the oceanic belt around Antarctica. 相似文献
947.
This paper describes the simulation of the flow of a viscous incompressible Newtonian liquid with a free surface. The Navier–Stokes equations are formulated using a streamline upwind Petrov–Galerkin scheme, and solved on a Q-tree-based finite element mesh that adapts to the moving free surface of the liquid. Special attention is given to fitting the mesh correctly to the free surface and solid wall boundaries. Fully non-linear free surface boundary conditions are implemented. Test cases include sloshing free surface motions in a rectangular tank and progressive waves over submerged cylinders. 相似文献
948.
本文分析了雷达观测结果,发现近海海面上的水汽向上输送随季节而变化,由于季节的变化和不同的天气情况,海表面大气现象在雷达中有不同的显示,根据这些显示得到了不同的信息,由此而得出春秋两季海表面水汽向上输送量的不同。 相似文献
949.
Xiaobo Chen 《Applied Ocean Research》1994,16(6):337-345
A numerical model based on using a tank Green function, has been developed to compute the side wall effects on first- and second-order loadings upon bodies of arbitrary geometry in wave tanks. This tank Green function (TGF) is composed of a finite series of open-sea Green functions and an asymptotic part represented by two single integrals whose kernels decrease exponentially with the integral variable. This consistent expression of the TGF permits one to highlight the side wall effects and to give some criteria for the choice of tank width and the measurement duration to limit the reflection of diffraction and radiation waves.
The efficiency of the developed model is shown in the application to hemispheres and a box-shaped barge placed in the center of the wave tanks. The numerical results explain well the irregularities in the experimental measurements and show that the side walls have important effects on the first-order quantities. These effects are much more pronounced on the second-order drift loads. 相似文献
950.
M. I. Fortus 《Izvestiya Atmospheric and Oceanic Physics》2007,43(5):555-568
In connection with the problem of revealing cause-effect relations among different climatic characteristics, methods for determining the dependence between time series on the basis of selected auto-and cross-covariance functions and periodograms expressed in terms of the Fourier coefficients of the initial series are discussed. Real time series do not always satisfy the conditions of statistical stationarity, so that their analysis requires a combination of statistical and deterministic approaches. The possibility of using the above numerical characteristics in relation to such series is considered. Characteristic features of describing finite segments of time series with the use of their Fourier coefficients is studied in detail. The main emphasis is on the determination of the time shifts (delays) at which the covariance between the series is maximal. The problems that appear during a practical implementation of the periodogram method for shift estimation are discussed. A previously unknown formula that is necessary for further studies and relates the Fourier transform of a selected correlation function to the periodogram of the series is derived. 相似文献