Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant     

Yano  Shin-ichi  Kawata  Yoshikazu  Kojima  Hiroyuki 《中国海洋湖沼学报》1998,16(1):105-108

A unicellular cyanobacteriumSynechococcus sp. strain PCC 7002 was transformed with plasmid pQL1, on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were encoded. The transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This result indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase, and accumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmotic shocks wihout, impairing cell viability. On the other hand, most of the β-galactosidase remained in cytoplasm under the osmotic shock.

  相似文献   

The fast multipole method for integral equations of seismic scattering problems     

Hiroyuki Fujiwara 《Geophysical Journal International》1998,133(3):773-782

  相似文献   

Deep and Bottom Currents in the Challenger Deep,Mariana Trench,Measured with Super-Deep Current Meters   总被引：3，自引：0，他引：3  

Keisuke?TairaEmail author  Shoji?Kitagawa  Toru?Yamashiro  Daigo?Yanagimoto 《Journal of Oceanography》2004,60(6):919-926

The bottom currents in the Challenger Deep, the deepest in the world, were measured with super-deep current meters moored at 11°22′ N and 142°35′ E, where the depth is 10915 m. Three current meters were set at 9687 m, 10489 m and 10890 m at the station in the center of the Challenger Deep for 442 days from 1 August 1995 to 16 October 1996. Although rotor revolutions in 60 minutes of recording interval were zero for 37.5% of the time, the maximum current at the deepest layer of 10890 m was 8.1 cm s⁻¹, being composed of tidal currents, inertia motion and long period variations. Two current meters were set at 6608 m and 7009 m at a station 24.9 km north of the center for 443 days from 31 July 1995 to 16 October 1996, and two current meters at 6214 m and 6615 m at a station 40.9 km south of the center for 441 days from 2 August 1995 to 16 October 1996. The mean flow at 7009 m depth at the northern station was 0.7 cm s⁻¹ to 240°T, and that at 6615 m depth at the southern station was 0.5 cm s⁻¹ to 267°T. A westward mean flow prevailed at the stations, and no cyclonic circulation with mean flows of the opposite directions was observed in the Mariana Trench at a longitude of 142°35′ E. Power spectra of daily mean currents showed three spectral peaks at periods of 100 days, 28–32 days and 14–15 days. The peak at 100 day period was common to the power spectra.  相似文献   

Storage-efficient reconstruction framework for planar contours     

Hiroyuki Goto  Yoichi Shimakawa 《地球空间信息科学学报》2017,20(1):14-28

A storage-efficient reconstruction framework for cartographic planar contours is developed. With a smaller number of control points, we aim to calculate the area and perimeter as well as to reconstruct a smooth curve. The input data forms an oriented contour, each control point of which consists of three values: the Cartesian coordinates (x, y) and tangent angle θ. Two types of interpolation methods are developed, one of which is based on an arc spline while the other one is on a cubic Hermite spline. The arc spline-based method reconstructs a G¹ continuous curve, with which the exact area and perimeter can be calculated. The benefit of using the Hermite spline-based method is that it can achieve G² continuity on most control points and can obtain the exact area, whereas the resulting perimeter is approximate. In a numerical experiment for analytically defined curves, more accurate computation of the area and perimeter was achieved with a smaller number of control points. In another experiment using a digital elevation model data, the reconstructed contours were smoother than those by a conventional method.  相似文献   

Measurements of ocean bottom pressure with a quartz sensor     

Keisuke Taira  Toshihiko Teramoto  Shoji Kitagawa 《Journal of Oceanography》1985,41(3):181-192

Year long measurements of bottom pressure were made at 2,036 m depth in Sagami Trough, at 2,538 m depth in Suruga Trough, and at 32 m depth in the south of Minami-Daitojima Island. Amplitudes and phase lags of the major constituents of tides were estimated by the response method, and they were compared with the observational results at several tide stations operated by the Japan Meteorological Agency. A comparison with Schwiderski's global models for the eight tidal constituents showed that the amplitudes were in good accordance to one another within 3 cm, and that the differences of phase lags were less than 15°. The largest portion of the variations of the bottom pressure was caused by the tides: the variance of the major eight constituents was more than 98.5% as large as the total variance. The measurements show that tidal waves can be recorded offshore with a sufficient accuracy by the quartz sensors. Drifts of indication of the pressure gauges were significant and they prevented detection of a long-term variation which might be caused by fluctuations of the ocean currents or by the eddies.  相似文献   

Preferential dissolution of SiO<Subscript>2</Subscript> from enstatite to H<Subscript>2</Subscript> fluid under high pressure and temperature     

Ayako Shinozaki  Hiroyuki Kagi  Hisako Hirai  Hiroaki Ohfuji  Taku Okada  Satoshi Nakano  Takehiko Yagi 《Physics and Chemistry of Minerals》2016,43(4):277-285

Stability and phase relations of coexisting enstatite and H₂ fluid were investigated in the pressure and temperature regions of 3.1–13.9 GPa and 1500–2000 K using laser-heated diamond-anvil cells. XRD measurements showed decomposition of enstatite upon heating to form forsterite, periclase, and coesite/stishovite. In the recovered samples, SiO₂ grains were found at the margin of the heating hot spot, suggesting that the SiO₂ component dissolved in the H₂ fluid during heating, then precipitated when its solubility decreased with decreasing temperature. Raman and infrared spectra of the coexisting fluid phase revealed that SiH₄ and H₂O molecules formed through the reaction between dissolved SiO₂ and H₂. In contrast, forsterite and periclase crystals were found within the hot spot, which were assumed to have replaced the initial orthoenstatite crystals without dissolution. Preferential dissolution of SiO₂ components of enstatite in H₂ fluid, as well as that observed in the forsterite H₂ system and the quartz H₂ system, implies that H₂-rich fluid enhances Mg/Si fractionation between the fluid and solid phases of mantle minerals.  相似文献   

Landslide Hazard and Risk Assessment on the Northern Slope of Mt. Changbai, China   总被引：1，自引：0，他引：1  

LIU Zhenghu  ZHANG Yanbin  Yoshiharu ISHIKAWA  Hiroyuki NAKAMURA 《《地质学报》英文版》2008,82(1):214-224

Landslide hazard and risk assessment on the northern slope of Mt. Changbai, a well-known tourist attraction near the North Korean-Chinese border, are assessed. This study is divided into two parts, namely, landslide hazard zonation and risk assessment. The 1992 Anbalagan and Singh method of landslide hazard zonation （LHZ） was modified and used in this area. In this way, an Associative Analysis Method was used in representative areas to get a measure for controlling factors （slope gradient, relative relief, vegetation, geology, discontinuity development, weak layer thickness and ground water）. For the membership degree of factor to slope failure, the middle range of limited values was used to calculate LHZ. Based on an estimation of the potential damage from slope failure, a reasonable risk assessment map was obtained using the relationship of potential damage and probable hazard to aid future planning and prediction and to avert loss of life.  相似文献   

Radon as an Indicator of Nocturnal Atmospheric Stability: A Simplified Theoretical Approach     

Yasutaka Omori  Hiroyuki Nagahama 《Boundary-Layer Meteorology》2016,158(2):351-359

  相似文献   

Projections of glacier change in the Altai Mountains under twenty-first century climate scenarios     

Yong Zhang  Hiroyuki Enomoto  Tetsuo Ohata  Hideyuki Kitabata  Tsutomu Kadota  Yukiko Hirabayashi 《Climate Dynamics》2016,47(9-10):2935-2953

  相似文献   

Data assimilation of sea ice concentration into a global ocean–sea ice model with corrections for atmospheric forcing and ocean temperature fields     

Takahiro Toyoda  Yosuke Fujii  Tamaki Yasuda  Norihisa Usui  Koji Ogawa  Tsurane Kuragano  Hiroyuki Tsujino  Masafumi Kamachi 《Journal of Oceanography》2016,72(2):235-262

  相似文献