The exploration and identification of antiproliferative phytochemicals have received increased attention in medicinal chemistry.In particular,research focused on the toxicology of marine natural products has increased in recent years.Terpenoids,among many secondary metabolites,have been demonstrated to act as effective anticancer agents.Soft corals,a group of marine invertebrates,produce a variety of terpenoids with biofunctional properties.The current study presents the extraction,purification,and identification of sterol congeners from the soft coral Dendronephthya putteri.The method involves 50%chloro form-methanol extraction,polar column fractionation,and analy sis through GC-MS~n.Do se-dependent antiproliferative activity was observed within the sterol-rich fraction(DPCMH 2-4),which consisted of3β-hydroxy-A5-steroidal congeners.This fraction inhibited the growth of HL-60 and MCF-7 cells with IC_(50) values of 25.27±1.43 and 22.81±0.15 μg/mL,respectively.Apoptotic body formation,DNA damage,cell cycle arrest,and apoptotic cell signaling pathway activation were also observed,reinforcing the dosedependent antiproliferative and apoptosis-inducing activity of 3β-hydroxy-A5-steroidal congeners.To our knowledge,this is the first report of anticancer agent identification from the soft coral D.putteri.Based on the observations,these steroidal congeners are promising candidates for the development of anticancer drugs. 相似文献
The results of long-term spectral monitoring of the Herbig Be star IL Cep A are presented. A smooth variation in the parameters of the Нα and Нβ line emission component has been discovered. The helium absorption lines He I λ4922 and 5876 Å show seasonal changes, however, these changes do not correlate with changes in hydrogen emission. The radial velocities of the narrow lines D Na I and DIB λ5780, 5796 Å correspond to that of the interstellar medium. The hydrogen emission lines are apparently formed in a triple system, including a close pair of B stars and a remote low-mass K–A component. The orbital elements of this system were calculated using an approximate spectral binary model, where a close pair of massive B stars was considered as one massive component. The rationale for this approach for determining the elements of the orbit is given. The orbital period P = 3550 ± 28 days is determined and some other parameters of the orbit are estimated. 相似文献
A thorough study on understanding of groundwater recharge sources and mechanisms was attempted by integrating the hydrogeological, geochemical and isotopic information along with groundwater dating and end-member mixing analysis (EMMA). This study was necessitated due to prolonged dryness and unavailability of freshwater in semi arid Deccan trap regions of Central India. In addition, groundwater resources are not characterized well in terms of their geochemical nature and recharge sources. The hydrogeochemical inferences suggest that aquifer I consists of recently recharged water dominated by Ca–Mg–HCO3 facies, while groundwater in aquifer II shows water–rock interaction and ion exchange processes. Presence of agricultural contaminant, nitrate, in both aquifers infers limited hydraulic interconnection, which is supported by unconfined to semi-confined nature of aquifers. Groundwater in both aquifers is unsaturated with respect to carbonate and sulfate minerals indicating lesser water–rock interaction and shorter residence time. This inference is corroborated by tritium age of groundwater (aquifer I: 0.7–2 years old and aquifer II: 2–4.2 years old). Stable water isotopes (δ2H, δ18O) suggest that groundwater is a mixture of rainwater and evaporated water (surface water and irrigation return flow). EMMA analysis indicates three groundwater recharge sources with irrigation return flow being the dominant source compared to others (rainwater and surface waters). A conceptual model depicting groundwater chemistry, recharge and dynamics is prepared based on the inferences.
Izvestiya, Atmospheric and Oceanic Physics - The results of using an original procedure for estimating albedo from the photogrammetric data and exposure parameters by an unmanned aerial vehicle... 相似文献
The unicellular cyanobacterium Synechocystis sp. PCC 6803, a model organism known for its unique combination of highly desirable molecular genetic, physiological and morphological characteristics, was employed in the present study. The species was cultured in BG11 liquid medium contained various initial concentrations of Pb2+ and Cd2+ (0, 0.5, 1, 2, 4, 6 and 8 mg/L). The experiment was conducted for six days and the metal induced alterations in the ultrastructure, growth and pigment contents were assessed. Alterations in the ultrastructure of the Synechocystis sp. PCC 6803 ceils became evident with the increased (>4 mg/L Pb2+) metal concentration. The photosynthetic apparatus (thylakoid membranes) were found to be the worst affected. Deteriorated or completely destroyed thylakoid membranes have made large empty spaces in the cell interior. In addition, at the highest concentration (8 mg/L pb2+), the polyphosphate granules became more prominent both in size and number. Despite the initial slight stimulations (0.2, 3.8 and 6.5% respectively at 0.5, 1 and 2 mg/L Pb2+), both metals inhibited the growth in a dose-dependent manner as incubation progressed. Pigment contents (chlorophyll a, βcarotene and phycocyanin) were also decreased with increasing metal concentration. Cells exposed to 6 mg/L Pb2+, resulted in 36.56, 37.39 and 29.34% reductions of chlorophyll a, β carotene and phycocyanin respectively over the control. Corresponding reductions for the same Cd2+concentrations were 57.83, 48.94 and 56.90%. Lethal concentration (96 h LC50) values (3.47 mg/L Cd2+ and 12.11 mg/L Pb2+) indicated that Synechocystis sp. PCC 6803 is more vulnerable to Cd2+ than Pb2+. 相似文献
A remote sensing-based approach was applied to study the impact of changes in cropping system on the exploitation of water
resources in two districts namely Ludhiana in central Punjab and Muktsar in South-Western Punjab. Rice-wheat remained dominant
rotation in Ludhiana while cottonwheat rotation was replaced partially by rice-wheat in Muktsar within a span of over five
years (1998–99 to 2003–04). The solo rice-wheat system in Ludhiana district has resulted in large-scale ground water exploitation
as is evident from the faster decline in water table (up to 0.9m year−1) and higher tube-wells density (440 per 1000 ha). As a result, nearly 60 per cent of the total area of Ludhiana district
has the water table depth greater than 10m and in some blocks, it has reached to a depth of 22 m. In Muktsar district, corresponding
rise in water table is 0.2m per year and tube well density is 114 per 1000 ha. Irrigation water associated with paddy cultivation
in Ludhiana and adjoining areas moves laterally through the buried paleo-channels of Sutlaj in the deeper soil profile and
gets accumulated in the basin lands of Muktsar and adjoining areas and causes an extra water flux and subsequent rise in water
table, recorded at 3 to 7m depth. To minimize the hydrological imbalance of the state, it is suggested to diversify some of
the area in the central districts from irrigation water intensive rice-wheat system to less water intensive cropping system. 相似文献
A series of experiments have been carried out in a developed liquid sloshing setup to estimate the pressure developed on the tank walls and the free surface displacement of water from the mean static level. The square tank attached to a shaking table can be moved to and fro by a cam arrangement driven by a DC motor. Pressure and displacement studies are done on the basis of changing excitation frequency of the shaking table and fill level in the tank. Experiments were carried out without and with baffles, and the consequent changes in the parameters are observed. 相似文献