Bacterioplanktonic abundance,productivity and petroleum hydrocarbon biodegradation in marinas and other coastal waters in North Carolina,USA     

Piehler MF  Maloney JS  Paerl HW 《Marine environmental research》2002,54(2):157-168

The purpose of this study was to assess the differences in the abundance and activity of the bacterioplankton at sites with varied boating activity, and to determine the response of the communities to additional petroleum pollution. Three sites, including two marinas and a site on Bogue Sound in coastal North Carolina, were selected for monthly experiments. Seasonal patterns of bacterioplanktonic abundance and diesel fuel biodegradation for each site were examined, and possible correlations with intensity of boating activities were explored. Bacterioplanktonic communities at the three sites in this study were more similar in their structure and biodegradation potential than would have been expected from previous studies. We found no differences in the abundance and biodegradation potential of theses communities at the three sites, and only a slightly elevated number of hydrocarbon degraders at one of the marinas. Patterns of biodegradation were more closely related to total bacterial abundance, rather than number of petroleum hydrocarbon degraders. There was a strong seasonal pattern in bacterial abundance and biodegradation at all three sites. The bacterioplanktonic Community at all sites responded similarly to additions of diesel fuel. Bacterial abundance and productivity were both elevated to some extent, and number of petroleum hydrocarbon degraders changed relatively little. These data indicate that marinas may not profoundly after the bacterioplankton and that coastal waters often have resident bacterial communities capable of degrading petroleum hydrocarbon pollution.  相似文献   

DISCUSSIONS ON STRUCTURAL COUPLING     

ZHU Wenbin~*  GUO Lingzhi  MA Ruishi  SUN Yan  WANG Feng 《大地构造与成矿学(英文版)》2004,28(1):1-17

The structural coupling is a common geological phenomenon. The structural differences between eastern and western active continental margins of modern Pacific and between paleo-Pacific and modern-Pacific continental margins are related to the characteristics and status of the subducting oceanic plate, namely, 1. subducting angle; 2. change in subducting angle; 3. subducting velocity; 4. change in subducting velocity; 5. subduction depth; 6. horizontal distance between the leading edge of the subducting plate and the trench; 7. the structural form of the subducting plate at the 670kin boundary between the upper and lower mantle; 8. the displacement and the direction of displacement of subducting plate. The control and influence toward the shallow-level structures by the deep-level structural activities is a detailed representation of the structural coupling on active continental margin. The basin-maintain coupling phenomenon is an intracontinental structural coupling. The far field effect of collision be  相似文献   

俄罗斯和乌克兰三个煤田中煤层甲烷开发和利用的机遇     

Mars.  JS 知叶 《国外煤田地质》1998,(4):19-29

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Molecular cloning and beta-naphthoflavone-induced expression of a cytochrome P450 1A (CYP1A) gene from an anadromous river pufferfish, Takifugu obscurus     

Kim JH  Raisuddin S  Ki JS  Lee JS  Han KN 《Marine pollution bulletin》2008,57(6-12):433-440

In recent years, there has been a decline in the wild populations of river pufferfish, Takifugu obscurus. Besides overexploitation for commercial purposes, environmental pollution is believed to have contributed to its decline. However, almost no information exists about genes involved in metabolism of xenobiotics by this species. Nevertheless, there is interest in fugu fishes, since they possess the smallest genome among vertebrates. We cloned and characterized the full-length cDNA sequence of a cytochrome P450 1A (CYP1A) gene from T. obscurus. Phylogenic relationship of T. obscurus CYP1A was also compared to other fish species. The tissue distribution and time-dependant induction of CYP1A mRNA were studied by real-time PCR in T. obscurus exposed to an aryl hydrocarbon receptor (Ahr) agonist, beta-naphthoflavone (BNF). The greatest basal expression in livers of control as well as BNF-treated individuals. However, brain, gill, gonad, intestine, and kidney also expressed CYP1A. Muscles expressed the least CYP1A. The results of the time-course study revealed induction in brain and gills after 6h and at 12h in most tissues. Except for gills, all other organs retained induced expression of CYP1A mRNA up to 96h.  相似文献   

Effects of land use on ground water quality in the Anoka Sand Plain Aquifer of Minnesota   总被引：6，自引：0，他引：6  

Trojan MD  Maloney JS  Stockinger JM  Eid EP  Lahtinen MJ 《Ground water》2003,41(4):482-492

We began a study, in 1996, to compare ground water quality under irrigated and nonirrigated agriculture, sewered and nonsewered residential developments, industrial, and nondeveloped land uses. Twenty-three monitoring wells were completed in the upper meter of an unconfined sand aquifer. Between 1997 and 2000, sampling occurred quarterly for major ions, trace inorganic chemicals, volatile organic compounds (VOCs), herbicides, and herbicide degradates. On single occasions, we collected samples for polynuclear aromatic hydrocarbons (PAHs), perchlorate, and coliform bacteria. We observed significant differences in water chemistry beneath different land uses. Concentrations of several trace inorganic chemicals were greatest under sewered urban areas. VOC detection frequencies were 100% in commercial areas, 52% in sewered residential areas, and <10% for other land uses. Median nitrate concentrations were greatest under irrigated agriculture (15,350 microg/L) and nonsewered residential areas (6080 microg/L). Herbicides and degradates of acetanilide and triazine herbicides were detected in 86% of samples from irrigated agricultural areas, 68% of samples from nonirrigated areas, and <10% of samples from other land uses. Degradates accounted for 96% of the reported herbicide mass. We did not observe seasonal differences in water chemistry, but observed trends in water chemistry when land use changes occurred. Our results show land use is the dominant factor affecting shallow ground water quality. Trend monitoring programs should focus on areas where land use is changing, while resource managers and planners must consider potential impacts of land use changes on ground water quality.  相似文献   

Recovery in rubble fields: long-term impacts of blast fishing     

Fox HE  Pet JS  Dahuri R  Caldwell RL 《Marine pollution bulletin》2003,46(8):1024-1031

This paper presents initial results from a study of factors that inhibit or enhance hard coral recovery in rubble fields created by blast fishing in Komodo National Park and Bunaken National Park, Indonesia. Within nine sites monitored since 1998, there was no significant natural recovery. Levels of potential source coral larvae were assessed with settlement tiles in the rubble fields and in nearby high coral cover sites. Rubble movement was measured and shown to be detrimental to small scleractinians, especially in high current areas. In shallow water (2-6 m deep), rubble is often overgrown by soft corals and corallimorpharians, which inhibit hard coral survival. There is increased scleractinian recruitment in quadrats cleared of soft coral, and Acropora nubbins transplanted into soft coral fields suffer greater mortality than those transplanted above the soft coral canopy. Gaining an understanding of the prognosis for coral recovery is essential not only in order to assess the long-term impacts of blast fishing, but also to improve management decisions about protection of intact reefs and potential restoration of damaged areas.  相似文献   

Biomagnification in marine systems: the perspective of an ecologist   总被引：14，自引：0，他引：14  

Gray JS 《Marine pollution bulletin》2002,45(1-12):46-52

Biomagnification is the process where xenobiotic substances are transferred from food to an organism resulting in higher concentrations compared with the source. It is widely believed that this is a general phenomenon for marine food webs. An analysis of 148 papers with biomagnification in the title shows that under half show biomagnification. Of studies on metals only organic mercury shows biomagnification and most metals are regulated and excreted and do not biomagnify. Of the studies on organic compounds 67% claimed to show biomagnification. However, bioconcentration (uptake from the surrounding water) is the most usual way that organic compounds are accumulated in organisms from invertebrates to and including fish. Only in sea-birds and marine mammals is food intake the major route and where biomagnification can be clearly shown. Body concentrations of organic compounds vary with lipid content and thus in order to compare across species normalisation to uniform lipid content should be done. Yet often this is not done so data purporting to show biomagnification merely relate to differing lipid content in the different species studied. Finally suggestions are made as to how data can be collected to better interpret the process of biomagnification in marine food webs.  相似文献   

Benthic diatom community response to environmental variables and metal concentrations in a contaminated bay adjacent to Casey Station, Antarctica     

Cunningham L  Snape I  Stark JS  Riddle MJ 《Marine pollution bulletin》2005,50(3):264-275

This study examined the effects of anthropogenic contaminants and environmental variables on the composition of benthic diatom communities within a contaminated bay adjacent to an abandoned waste disposal site in Antarctica. The combination of geographical, environmental and chemical data included in the study explained all of the variation observed within the diatom communities. The chemical data, particularly metal concentrations, explained 45.9% of variation in the diatom communities, once the effects of grain-size and spatial structure had been excluded. Of the metals, tin explained the greatest proportion of variation in the diatom communities (28%). Tin was very highly correlated (R2>0.95) with several other variables (copper, iron, lead, and sum of metals), all of which explained similarly high proportions of total variation. Grain-size data explained 23% of variation once the effects of spatial structure and the chemical data had been excluded. The pure spatial component explained only 1.8% of the total variance. The study demonstrates that much of the compositional variability observed in the bay can be explained by concentrations of metal contaminants.  相似文献   

Cloning and expression of alpha class glutathione S-transferase gene from the small hermaphroditic fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)     

Lee YM  Chang SY  Jung SO  Kweon HS  Lee JS 《Marine pollution bulletin》2005,51(8-12):776-783

In order to assess its potential as a biomarker of aquatic pollution, an alpha class glutathione S-transferase gene (GSTalpha gene) was cloned from the small hermaphroditic fish Rivulus marmoratus. The R. marmoratus GSTalpha gene spanned 1.3 kb, consisting of 6 exons encoding 221 amino acid residues. It showed high similarity to zebrafish GST. We named this R. marmoratus GSTalpha gene as rm-GSTalpha. The cDNA of the rm-GSTalpha gene was also investigated for its phylogeny, tissue-specific and chemical-induced expression. Rm-GSTalpha was subcloned into a 6 x His-tagged pCRT7 TOPO TA expression vector to produce the recombinant 6 x His-tagged rm-GST protein. This will be used in future to raise an rm-GSTalpha antibody for use in the study of phase II metabolism involved in detoxification. We also exposed R. marmoratus to 300 microg/l of 4-nonylphenol in water, and found approximately 4-fold induction of R. marmoratus GSTalpha mRNA in the treated animals. In this paper, we discuss the characteristics of the R. marmoratus GSTalpha gene as well as its potential use in relation to environmental pollution.  相似文献   

Long-term marine litter monitoring in the remote Great Australian Bight, South Australia     

Edyvane KS  Dalgetty A  Hone PW  Higham JS  Wace NM 《Marine pollution bulletin》2004,48(11-12):1060-1075

The Anxious Bay beach litter clearance is the longest running annual survey of ocean-based litter in Australia. It's remoteness from centres of human population and location (with respect to prevailing winds and currents) make it an ideal place for monitoring ocean or ship-based litter in Australia's southern oceans and particularly, the Great Australian Bight. Over the 1991-1999 period, a large but gradual decline in the amount of beach washed litter was recorded (with minor peaks recorded during the 1992 and 1994 surveys). Beach washed litter decreased by approximately 86%, from 344 kg recorded in 1991 (13.2 kg/km) to 49 kg in 1999 (i.e. 1.9 kg/km), reaching a maximum of 390 kg in 1992 (or 15 kg/km of beach). However, a sharp increase in litter was recorded in 2000 (i.e. 252 kg or 9.7 kg/km). This increase in litter yield in 2000 is probably due to stronger than average onshore surface flow (or Ekman Transport) in the western Eyre Peninsula and Bight region. Prior to the survey in 2000, the results appeared to indicate that ocean litter on Anxious Bay beach was beginning to level out at around 50-70 kg/year (i.e. 2-3 kg/km). As the beach surveys involve the assumption that the beach is completely cleared of litter, this may represent a baseline level for ocean-based litter in the region. The yields and type of litter collected from the annual survey indicates that the majority of litter washed ashore originates from commercial fishing activities within the Great Australian Bight. Most of the fishing-related litter was directly sourced to the Southern Rock Lobster Fishery (i.e. bait buckets, baskets, pots), the Great Australian Bight Trawl Fishery (i.e. codends, trawl nets) and the Southern Shark Fishery (i.e. monofilament gillnets and longlines). Between 1994 and 1999, large reductions were observed in the amount of bait straps (77% reduction), lobster bait baskets/buckets (86% reduction), nets/ropes (62% reduction) and floats/buoys (83% reduction). Significantly, fishing-related litter in the Bight has reduced at a slower rate than domestic litter. While the level of glass and soft plastics on the beach have both reduced by almost 93% (i.e. 103-7 kg and 119-8 kg, respectively), the level of hard plastics, has diminished at a slower rate, with reductions of only 75% (i.e. 122-30 kg). Some fisheries (i.e. rock lobster, Southern Shark Fishery) have shown marked reductions in fishing-related litter. This is probably due, to some extent, to significant reductions in fishing effort in the region, although this requires further investigation. The information from the Anxious Bay beach litter survey is crucial in monitoring trends in ocean litter in Australia's southern oceans and compliance with international litter regulations. While fishing-related litter remains the major source of ship-based or ocean litter in Australia's southern oceans, the continued reduction in ship-based litter since 1991 supports increasing compliance to MARPOL (Annex V) by commercial fisheries and shipping in the Great Australian Bight. While Australia participates in marine debris monitoring programs in the Antarctic (under CCAMLR), there is currently no national program or management framework to assess, manage and monitor ocean-based litter along Australia's coasts, and monitor compliance with MARPOL. Apart from the commitments under CCAMLR for Antarctic (and sub-Antarctic) marine environments, there are no other regional programs, guidelines or monitoring protocols or to assess and manage ocean litter in the Southern Ocean.  相似文献