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Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
13.
李权昆 《广东海洋大学学报》2002,22(5):35-38
现行农村信用社存在的出资人所有权虚置、收益权削弱、法人财产权丧失等产权制度缺陷 ,严重阻碍着它的进一步发展。形成这些缺陷的主要原因在于农村信用社产权改革的方向不明和对产权范畴的片面理解 ,因此 ,农村信用社产权制度创新应在清晰界定产权的基础上、向股份制、股份合作制、合作制等多种产权形式的方向发展。 相似文献
14.
The mass ratio of Charon to Pluto is a basic parameter describing the binary system and is necessary for determining the individual masses and densities of these two bodies. Previous measurements of the mass ratio have been made, but the solutions differ significantly (Null et al., 1993; Young et al., 1994; Null and Owen, 1996; Foust et al., 1997; Tholen and Buie, 1997). We present the first observations of Pluto and Charon with a well-calibrated astrometric instrument—the fine guidance sensors on the Hubble Space Telescope. We observed the motion of Pluto and Charon about the system barycenter over 4.4 days (69% of an orbital period) and determined the mass ratio to be 0.122±0.008 which implies a density of 1.8 to 2.1 g cm−3 for Pluto and 1.6 to 1.8 g cm−3 for Charon. The resulting rock-mass fractions for Pluto and Charon are higher than expected for bodies formed in the outer solar nebula, possibly indicating significant postaccretion loss of volatiles. 相似文献
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Earth, Moon, and Planets - 相似文献
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The Cassini spacecraft, en route to Saturn, passed close to Jupiter while the Galileo spacecraft was completing its 28th and 29th orbits of Jupiter, thus offering a unique opportunity for direct study of the solar wind-Jovian interaction. Here evidence is given of response of the Jovian magnetopause and bow shock positions to changes of the north-south component of the solar wind magnetic field, a phenomenon long known to occur in equivalent circumstances at Earth. The period analyzed starts with the passage over Cassini of an interplanetary shock far upstream of Jupiter. The shock's arrival at Galileo on the dusk-flank of the magnetosphere caused Galileo to exit into the solar wind. Using inter-spacecraft timing based on the time delay established from the shock arrival at each spacecraft, we point out that Galileo's position with respect to the Jovian bow shock appears to correlate with changes in the disturbed north-south reversing field seen behind the shock. We specifically rule out the alternative of changes in the shape of the bow shock with rotations of the interplanetary magnetic field as the cause. 相似文献
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The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin. 相似文献
20.
The kinetics of the reactions of C2H radical with ethane (k1), propane (k2), and n-butane (k3) are studied over the temperature range of T = 96-296 K with a pulsed Laval nozzle apparatus that utilizes a pulsed laser photolysis-chemiluminescence technique. The C2H decay profiles in the presence of both the alkane reactant and O2 are monitored by the CH(A2Δ) chemiluminescence tracer method. The results, together with available literature data, yield the following Arrhenius expressions: k1(T) = (0.51 ± 0.06) × 10−10 exp[(−76 ± 30)K/T] cm3 molecule−1 s−1 (T = 96-800 K), k2(T) = (0.98 ± 0.32) × 10−10exp[(−71 ± 60)K/T] cm3 molecule−1 s−1 (T = 96-361 K), and k3(T) = (1.23 ± 0.26) × 10−10 cm3 molecule−1 s−1 (T = 96-297 K). At T = 296 K, k1 is measured as a function of total pressure and has little or no pressure dependence. The results from this work support a direct hydrogen abstraction mechanism for the title reactions. Implications to the atmospheric chemistry of Titan are discussed. 相似文献