A comparison of cytochrome P4501A (CYP1A) mRNA inducibility in four species of Atlantic coast anadromous fishes     

Isaac Wirgin  Blake Konkle  Mark Pedersen  Cheryl Grunwald  Jim Williams  Simon C. Courtenay 《Estuaries and Coasts》1996,19(4):913-922

Quantification of levels of cytochrome P4501A (CYP1A) gene expression in sentinel species of fishes has been proposed as a management tool to evaluate contamination of aquatic systems. Based on preliminary studies, we hypothesized that differences in CYP1A mRNA inducibility among individuals, populations, or species might lead to spurious conclusions when using this approach in environmental monitoring programs. To address this possibility, we quantitated and compared CYP1A mRNA induction levels in four species of common Atlantic Coast estuarine fish: smooth flounder, hogchoker, striped bass, and Atlantic tomcod, which were treated with model chemicals (beta naphthoflavone (β-NF), or benzo[a]pyrene at 10 ppm) known to induce CYP1A mRNA, or were exposed to contaminated environments. Species-specific CYP1A DNA probes were generated from PCR (polymerase chain reaction) amplification of genomic DNA using conserved oligonucleotide primers, and, along with cloned rainbow trout and Atlantic tomcod CYP1A cDNA probes were used to quantify CYP1A mRNA levels in northern blot analyses. Successful PCR amplification of CYP1A hybridizable DNA fragments was observed for all four species. Results from northern blot analyses showed large differences in CYP1A mRNA induction among species; only Atlantic tomcod exhibited significant induction of CYP1A mRNA for both chemically treated (97-fold) and environmentally exposed fish (34-fold). Significant, although lower, levels of induction were observed in β-NF treated (14-fold) smooth flounder, but not in environmentally exposed smooth flounder. Only low levels (not significant) of CYP1A gene induction were detected in hogchokers and striped bass. We conclude that CYP1A mRNA inducibility differed significantly among fish taxa perhaps due to differences in regulation of gene expression, suggesting that careful selection of sentinel species should be exercised prior to the use of CYP1A mRNA induction in environmental monitoring programs. However, the significance of differences in CYP1A mRNA inducibility in relation to higher level biological endpoints has yet to be determined.  相似文献   

Post-collapse volcanic history of calderas on a composite volcano: an example from Roccamonfina,southern Italy     

P D Cole  J E Guest  A M Duncan  D K Chester  R Bianchi 《Bulletin of Volcanology》1992,54(4):253-266

Roccamonfina, part of the Roman Potassic Volcanic Province, is an example of a composite volcano with a complex history of caldera development. The main caldera truncates a cone constructed predominantly of this caldera may have been associated with one of the ignimbritic eruptions of the Brown Leucitic Tuff (BLT) around 385 000 yr BP. The Campagnola Tuff, the youngest ignimbrite of the BLT, however, drapes the caldera margin and must postdate at least the initial stages of collapse. During the subsequent history of the caldera there were several major explosive eruptions. The largest of these was that of the Galluccio Tuff at about 300 000 yr BP. It is likely that there was further collapse within the main caldera associated with these eruptions. It is of note that despite these subsequent major explosive eruptions later collapse occurred within the confines of the main caldera. Between eruptions caldera lakes developed producing numerous lacustrine beds within the caldera fill. Extensive phases of phreatomagmatic activity generated thick sequences of pyroclastic surge and fall deposits. Activity within the main caldera ended with the growth of a large complex of basaltic trachyandestite lava domes around 150 000 yr BP. Early in the history of Roccamonfina sector collapse on the northern flank of the volcano formed the northern caldera. One of the youngest major events on Roccamonfina occurred at the head of this northern caldera with explosive activity producing the Conca Ignimbrite and associated caldera. There is no evidence that there was any linkage in the plumbing systems that fed eruptions in the main and northern calderas.  相似文献   

Water Quality Response to the Partial Casing of a Well     

Tim Grundl  Jim Delwiche 《Ground water》1992,30(1):45-49

  相似文献   

Case Histories of Shallow Time Domain Electromagnetics in Environmental Site Assessment     

Pieter Hoekstra  Raye Lahti  Jim Hild  C. Richard Bates  David Phillips 《Ground Water Monitoring & Remediation》1992,12(4):110-117

Time domain electromagnetic (TDEM) soundings were utilized in site assessment studies for the purpose of accomplishing two objectives: (1) measuring the migration of brines from oil and gas field evaporation pits, and (2) mapping the continuity of clay strata. Both case histories are representative of common objectives in site assessment. The data for both examples were analyzed by one-dimensional ridge regression inversions.
Both case histories illustrate that TDEM is an effective method for determining the lateral and vertical resistivity distribution (geoelectric section) of the subsurface in the depth range from about 5 m to 100 m, and it is known from other investigations that depths of investigations up to 2500 m can be achieved with TDEM.
Frequently, the lateral extent of contaminant plumes emanating from localized sources are of limited areal extent. The case history involving the use of TDEM to map a brine plume shows that a TDEM measurement has a relatively small zone of influence, so that meaningful information about the lateral and vertical extent of the plume can be obtained. Both case histories demonstrate the ability of TDEM to determine geoelectric sections below layers of low resistivity.  相似文献   

The transformation and export of phosphorus from Wetlands     

Jim Gehrels  George Mulamoottil 《水文研究》1989,3(4):365-370

There is widespread acceptance of the phosphorus retention capability of wetlands even though research findings are often inconclusive and contradictory. The results of a one year phosphorus budget study indicate that internal wetland processes may transform sediment bound phosphorus to plant available orthophosphorus. While total phosphorus imports were nearly double the total phosphorus exports for the study wetland, orthophosphorus exports were 22 per cent greater than imports. This study supports the recent finding that wetlands have limited capability to retain orthophosphorus and indicates that wetlands may even increase the export of orthophosphorus. The generally accepted nutrient retention function of wetlands and their possible role in eutrophication is thus questionable.  相似文献   

Electrodynamic heating and movement of the thermosphere     

K.D. Cole 《Planetary and Space Science》1971

The theory of dissipation of ionospheric electric currents is extended to include viscosity. In a steady state (i.e. usually above about 140 km altitude) the joule plus viscous heating may be calculated by μ∇²v. E × B/B². At lower altitudes where viscosity may, in some circumstances, be relatively unimportant the joule dissipation is calculated by the usual formula j. (E + v × B). In a prevalent model of the auroral electrojets it is found that the joule heating can be much more intense outside auroral forms than within them. Heating due to auroral electrojets cause a semi-annual variation in the thermosphere. Movement caused by auroral electric fields make a contribution to the super-rotation of the midlatitude upper atmosphere. Random electric fields lead to an eddy ‘viscosity’ or ‘exchange coefficientrs in the upper thermosphere of magnitude ρE_R²/B³t_R²|∇E|. where t_R is the correlation time of the random component of electric fields E_R and ρ is air density. Theoretical conditions for significant heating by field-aligned currents are derived.  相似文献   

Cheap foreign labour: empire relic with no place in civilized society     

Jim Slater 《Marine Policy》1984,8(1):64-65

  相似文献   

Distances from auroral zones to the magnetic and geographic equators     

K.D. Cole  P.F.B. Williams 《Planetary and Space Science》1982,30(10):1073-1076

Distance from auroral zone is a fundamental parameter in studies of disturbances produced in the thermosphere and ionosphere through the action of the solar wind. Calculations showing the great variation of the distances of the auroral “zones” from the magnetic equator and geographic equator are presented in diagrams. An auroral zone proximity index is proposed for use in correlative studies of upper atmosphere and of ionospheric disturbances.  相似文献   

Comparison of heat and bromide as ground water tracers near streams   总被引：3，自引：0，他引：3  

Constantz J  Cox MH  Su GW 《Ground water》2003,41(5):647-656

Heat and bromide were compared as tracers for examining stream/ground water exchanges along the middle reaches of the Santa Clara River, California, during a 10-hour surface water sodium bromide injection test. Three cross sections that comprise six shallow (<1 m) piezometers were installed at the upper, middle, and lower sections of a 17 km long study reach, to monitor temperatures and bromide concentrations in the shallow ground water beneath the stream. A heat and ground water transport simulation model and a closely related solute and ground water transport simulation model were matched up for comparison of simulated and observed temperatures and bromide concentrations in the streambed. Vertical, one-dimensional simulations of sediment temperature were fitted to observed temperature results, to yield apparent streambed hydraulic conductivities in each cross section. The temperature-based hydraulic conductivities were assigned to a solute and ground water transport model to predict sediment bromide concentrations, during the sodium bromide injection test. Vertical, one-dimensional simulations of bromide concentrations in the sediments yielded a good match to the observed bromide concentrations, without adjustment of any model parameters except solute dispersivities. This indicates that, for the spatial and temporal scales examined on the Santa Clara River, the use of heat and bromide as tracers provide comparable information with respect to apparent hydraulic conductivities and fluxes for sediments near streams. In other settings, caution should be used due to differences in the nature of conservative (bromide) versus nonconservative (heat) tracers, particularly when preferential flowpaths are present.  相似文献   

Look out, aurorae about!     

Dr  Jim Wild 《Astronomy& Geophysics》2006,47(1):1.11-1.13

Jim Wild recounts the rationale and success of AuroraWatch, a service that alerts you when you might be able to see aurorae.  相似文献