Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

板块构造和远洋相：晚侏罗世...     

Decker  K 刘健 《海洋地质译丛》1991,(1):41-48

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加里曼丹沙巴西北陆缘的逆掩构造     

Hinz.  K 周效中 《南海研究与开发》1991,(2):63-73

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由偏顶蛤贝壳中稳定同位素剖面追溯海水温度记录     

蔡德陵  A.Margosian  F.C.Tan  K.H.Mann 《海洋科学进展》1988,(3)

本文用在加拿大诺瓦·斯科舍省芬克湾采集到的两只活体偏顶蛤(Modiolus)贝壳上的氧同位素组成重建了过去十年间的底层海水温度记录,它也可作为一种独立的方法来确定贝壳上外生长线的沉积时间。由于对贝壳进行了密集采样(可多至每个贝壳沉积年份取15个样品),贝壳上的δ~(16)O变化反映了芬克湾底层海水的季节性变化。两只贝壳所得的温度剖面符合得很好,说明它们基本上是与真实的环境温度相对应的。本研究证明在海胆疾病爆发时底层海水的温度并没有异常升高,这说明疾病传播的关键因素是带病的生物体,而不是温度。同位素技术也说明了在贝壳外表面上可见的外生长线是年轮性的,它们是在每年冬季最低水温时沉积出来的。  相似文献   

A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians   总被引：2，自引：0，他引：2  

Azumi K  Fujie M  Usami T  Miki Y  Satoh N 《Marine environmental research》2004,58(2-5):543-546

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.  相似文献   

伊豆—小笠原海沟之西迁和菲律宾海板块之北...     

Otsuki  K 赵国先 《海洋地质译丛》1991,(6):9-17

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Impacts of stormwater discharges on the nearshore benthic environment of Santa Monica Bay     

Schiff K  Bay S 《Marine environmental research》2003,56(1-2):225-243

Although large loads of potentially toxic constituents are discharged from coastal urban watersheds, very little is known about the fates and eventual impacts of these stormwater inputs once they enter the ocean. The goal of this study was to examine the effects of stormwater discharges on the benthic marine environment of Santa Monica Bay. Sediment samples were collected across a gradient of stormwater impact following significantly sized storm events offshore Ballona Creek (a predominantly developed watershed) and Malibu Creek (a predominantly undeveloped watershed). Sediments offshore Malibu Creek had a greater proportion of fine-grained sediments, organic carbon, and naturally occurring metals (i.e., aluminum and iron), whereas sediments offshore Ballona Creek had higher concentrations of anthropogenic metals (i.e., lead) and organic pollutants (i.e., total DDT, total PCB, total PAH). The accumulation of anthropogenic sediment contaminants offshore Ballona Creek was evident up to 2 km downcoast and 4 km upcoast from the creek mouth and sediment concentrations covaried with distance from the discharge. Although changes in sediment texture, organic content, and an increase in sediment contamination were observed, there was little or no alteration to the benthic communities offshore either Ballona or Malibu Creek. Both sites were characterized as having an abundance, species richness, biodiversity and benthic response index similar to shallow water areas distant from creek mouths throughout the Southern California Bight. There was not a preponderance of pollution tolerant, nor a lack or pollution sensitive, species offshore either creek mouth.  相似文献   

东营市东辛2-4井磁性地层划分对比研究   总被引：6，自引：1，他引：6  

周墨清  葛宗诗  郭青霞  姚益民  任发琛  何礼章  卢永顺 《海洋地质与第四纪地质》1992,(3)

本文通过东辛2—4井岩心样品的古地磁学研究,整段岩心呈现以正极性为主的混合极性特征。自上而下划分的布容、松山、高斯和吉尔伯特极性时和布容极性时内清晰显示出的哥德堡和布莱克亚时的界限分别位于井深28.0m、161.0m、274.5m和452.0m。据黄河口南北5个钻孔磁性地层的划分对比,长江口、苏北盆地和东营地区,吉尔伯特、高斯和布容极性时的沉积速度较快,松山极性时较慢或近于缺失;黄河口以北至河北地区,高斯极性时地层不发育,吉尔伯特、松山和布容极性时则较厚,表明它们所处的构造单元、地质基础和沉积环境有较大的不同。  相似文献   

盐渍土壤的介电特性     

Sree.  K 《海岸工程》1996,15(4):81-85

采用Ｌ波段介电探针，对沙壤，砂质粘土和粘土三种不同性质的土壤作了详尽的实验室实验，结果显示，土壤的盐度同介电常数的实无关，而同虚部相关，并随盐度的增加而增加。还表明，实数部分更多的依赖于土壤的湿度，虚数部分取决地土壤的盐度，土壤的含钠性对介电常数的实部和虚部都无影响。本研究展示了Ｌ波段对土壤盐度的微波响应。同时说明，在湿壤的条件下，采用Ｌ波段，将盐壤从钠壤中分离出来的可能性。  相似文献   

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis   总被引：3，自引：1，他引：3  

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2)

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po  相似文献