Inverse kinetic isotope fractionation during bacterial nitrite oxidation   总被引：4，自引：0，他引：4  

Karen L. Casciotti 《Geochimica et cosmochimica acta》2009,73(7):2061-2076

Natural abundance stable isotopes in nitrate (), nitrite (), and nitrous oxide (N₂O) have been used to better understand the cycling of nitrogen in marine and terrestrial environments. However, in order to extract the greatest information from the distributions of these isotopic species, the kinetic isotope effects for each of the relevant microbial reactions are needed. To date, kinetic isotope effects for nitrite oxidation and anaerobic ammonium oxidation (anammox) have not been reported. In this study, the nitrogen isotope effect was measured for microbial nitrite oxidation to nitrate. Nitrite oxidation is the second step in the nitrification process, and it plays a key role in the regeneration of nitrate in the ocean. Surprisingly, nitrite oxidation occurred with an inverse kinetic isotope effect, such that the residual nitrite became progressively depleted in ¹⁵N as the reaction proceeded. Three potential explanations for this apparent inverse kinetic isotope effect were explored: (1) isotope exchange equilibrium between nitrite and nitrous acid prior to reaction, (2) reaction reversibility at the enzyme level, and (3) true inverse kinetic fractionation. Comparison of experimental data to ab initio calculations and theoretical predictions leads to the conclusion that the fractionation is most likely inverse at the enzyme level. Inverse kinetic isotope effects are rare, but the experimental observations reported here agree with kinetic isotope theory for this simple N-O bond-forming reaction. Nitrite oxidation is therefore fundamentally different from all other microbial processes in which N isotope fractionation has been studied. The unique kinetic isotope effect for nitrite oxidation should help to better identify its role in the cycling of nitrite in ocean suboxic zones, and other environments in which nitrite accumulates.  相似文献   

Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin     

Suzan Pantaroto de Vasconcellos  Elaine Crespim  Georgiana Feitosa da Cruz  Diego Barbosa Senatore  Karen Christina Marques Simioni  Eugênio Vaz dos Santos Neto  Anita Jocelyne Marsaioli  Valéria Maia de Oliveira 《Organic Geochemistry》2009,40(5):574-588

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.  相似文献   

Late Pleistocene stratigraphy and sedimentary environments of the Severnaya Dvina‐Vychegda region in northwestern Russia     

Astrid Lyså  Eiliv Larsen  Jan‐Pieter Buylaert  Ola Fredin  Maria A. Jensen  Denis Kuznetsov  Andrew S. Murray  Dmitry A. Subetto  Aurelien van Welden 《Boreas: An International Journal of Quaternary Research》2014,43(4):759-779

The Late Pleistocene stratigraphy from the Severnaya Dvina‐Vychegda region of northwestern Russia is revised based on investigations of new localities, revisiting earlier localities, introduction of about 110 new OSL dates and burial depth corrections of earlier published OSL dates, in addition to six new radiocarbon dates. Most of the OSL samples studied here are from fluvial and subaquaeous sediments, which we found to be well bleached. Six chronostratigraphical units and their sedimentary environment are described, with the oldest unit consisting of pre‐Eemian glacial beds. For the first time, Early Weichselian sediments are documented from the region and a fluvial environment with some vegetation and permafrost conditions is suggested to have persisted from the end of the Eemian until at least about 92 ka ago. The period in which a Middle Weichselian White Sea Lake could have existed is constrained to 67?62 ka, but as the lake level never reached the thresholds of the drainage basin, the lake probably existed only for a short interval within this time‐span. Blocking and reversal of fluvial drainage started again around 21?20 ka ago when the Fennoscandian Ice Sheet advanced into the area, reaching its maximum 17?15 ka ago. At that time, an ice‐dammed lake reached its maximum water level, which was around 135 m above present sea level. Drainage of the lake started shortly after 15 ka ago, and the lake was emptied within 700 years. Severe periglacial conditions, with permafrost and aeolian activity, prevailed in the area until about 10.7 ka.  相似文献   

Simplification and Validation of a Spectral-Tensor Model for Turbulence Including Atmospheric Stability   总被引：1，自引：1，他引：0  

Abhijit Chougule  Jakob Mann  Mark Kelly  Gunner C. Larsen 《Boundary-Layer Meteorology》2018,167(3):371-397

A spectral-tensor model of non-neutral, atmospheric-boundary-layer turbulence is evaluated using Eulerian statistics from single-point measurements of the wind speed and temperature at heights up to 100 m, assuming constant vertical gradients of mean wind speed and temperature. The model has been previously described in terms of the dissipation rate \(\epsilon \), the length scale of energy-containing eddies \(\mathcal {L}\), a turbulence anisotropy parameter \(\varGamma \), the Richardson number Ri, and the normalized rate of destruction of temperature variance \(\eta _\theta \equiv \epsilon _\theta /\epsilon \). Here, the latter two parameters are collapsed into a single atmospheric stability parameter z / L using Monin–Obukhov similarity theory, where z is the height above the Earth’s surface, and L is the Obukhov length corresponding to \(\{Ri,\eta _\theta \}\). Model outputs of the one-dimensional velocity spectra, as well as cospectra of the streamwise and/or vertical velocity components, and/or temperature, and cross-spectra for the spatial separation of all three velocity components and temperature, are compared with measurements. As a function of the four model parameters, spectra and cospectra are reproduced quite well, but horizontal temperature fluxes are slightly underestimated in stable conditions. In moderately unstable stratification, our model reproduces spectra only up to a scale \(\sim \) 1 km. The model also overestimates coherences for vertical separations, but is less severe in unstable than in stable cases.  相似文献   

Climate-smart agriculture: perspectives and framings     

Alvin Chandra  Karen E. McNamara  Paul Dargusch 《Climate Policy》2018,18(4):526-541

This paper offers a systematic analysis of the concepts and contexts that frame the climate-smart agriculture (CSA) discourse in the academic and policy literature. Documents (n?=?113) related to CSA and published in peer-reviewed journals, books, working papers, and scientific reports from 2004 to 2016 were reviewed. Three key trends emerged from the analysis: studies are biased towards global policy agendas; research focuses on scientific and technical issues; and the integration of mitigation, adaptation, and food security (the three pillars of CSA) is becoming a popular scholarly solution. Findings suggest that CSA is a fairly new concept used to describe a range of adaptation and mitigation practices without a specific set of criteria. Although CSA is often framed around the three pillars, the underlying issues constructing the discourse differ at global, developing, and developed country scales. Although there is increasing research on developing countries, particularly in relation to how CSA can transform smallholder agriculture, there is a paucity of research documenting the experiences from developed countries. The findings suggest that research on CSA needs to move beyond solely focussing on scientific approaches and only in certain geographical contexts. If CSA is to be applicable for farmers across the globe, then cross-disciplinary research that is underpinned by broad socio-economic and political contexts is essential to understand how differences in narratives might affect implementation on-the-ground in both developing and developed countries.

POLICY RELEVANCE

Although policy makers are increasingly supportive of the climate-smart agriculture (CSA) approach, the rhetoric has largely been developed on the basis of scientific and technical arguments. The political implications of varying perspectives have resulted in a growing divide between how developing and developed countries frame solutions to the impacts of climate change on agriculture under the 2015 Paris Agreement. Different framings are part of the explanation for why the scope of CSA is being rethought, with the scientific community redirecting attention to seeking a separate work programme under the United Nations Framework Convention on Climate Change (UNFCCC). The current policy framing of CSA will give no new policy direction unless it grounds itself in the smallholder farmer and civil society contexts.  相似文献   

Learning from CDM SD tool experience for Article 6.4 of the Paris Agreement     

Karen Holm Olsen  Christof Arens  Florian Mersmann 《Climate Policy》2018,18(4):383-395

The Paris Agreement (PA) emphasizes the intrinsic relationship between climate change and sustainable development (SD) and welcomes the 2030 agenda for the global Sustainable Development Goals (SDGs). Yet, there is a lack of assessment approaches to ensure that climate and development goals are achieved in an integrated fashion and trade-offs avoided. Article 6.4 of the PA introduces a new Sustainable Mitigation Mechanism (SMM) with the dual aim to contribute to the mitigation of greenhouse gas emissions and foster SD. The Kyoto Protocol’s Clean Development Mechanism (CDM) has a similar objective and in 2014, the CDM SD tool was launched by the Executive Board of the CDM to highlight the SD benefits of CDM activities. This article analyses the usefulness of the CDM SD tool for stakeholders and compares the SD tool’s SD reporting requirements against other flexible mechanisms and multilateral standards to provide recommendations for improvement. A key conclusion is that the Paris Agreement’s SMM has a stronger political mandate than the CDM to measure that SD impacts are ‘real, measurable and long-term’. Recommendations for an improved CDM SD tool are a relevant starting point to develop rules, modalities, and procedures for SD assessment in Article 6.4 as well as for other cooperative mitigation approaches.

POLICY RELEVANCE

Research findings are relevant for developing the rulebook of modalities and procedures for Article 6.4 of the Paris Agreement, which introduces a new mechanism for mitigation of greenhouse gas emissions and sustainable development. Lessons learnt from the CDM SD tool and recommendations for enhanced SD assessment are discussed in context of Article 6 cooperative approaches, and make a timely contribution to inform negotiations on the rulebook agreed by the Conference of the Parties serving as the Meeting of the Parties to the Paris Agreement.  相似文献   

Taming a “chaotic concept”: gentrification and segmented consumption in Brooklyn, 2002–2012     

Mike Owen Benediktsson  Brian Lamberta  Erika Larsen 《Urban geography》2016,37(4):590-610

Public debate and prior scholarship tend to emphasize the link between gentrification and high-end chain retail, underscoring the importance of taste cultures tied to social class in making sense of gentrification’s impact on neighborhood identity. In this study, we present evidence from 10 years of change in the businesses of Brooklyn, New York City, a period of extensive gentrification across a wide variety of neighborhood and census-tract level contexts. Adopting census tracts clustered in neighborhoods as the units of analysis, we model the effects of institutional and demographic change on two separate outcomes: chain retail density and homogeneity in the types of goods and services available. We argue that changes in consumer culture embedded in broader processes of gentrification are neither “chaotic” nor “unitary” but are “segmented” according to local spatial and demographic context, taking two discrete forms: institutionally facilitated corporatization; lifestyle-driven homogenization.  相似文献   

Does Magnetic Flux Submerge at Flux Cancelation Sites?     

Harvey  Karen L.  Jones  Harrison P.  Schrijver  Carolus J.  Penn  Matthew J. 《Solar physics》1999,190(1-2):35-44

Simultaneous measurements of the magnetic fields in the photosphere and chromosphere were used to investigate if magnetic flux is submerging at sites between adjacent opposite polarity magnetic network elements in which the flux is observed to decrease or `cancel'. These data were compared with chromospheric and coronal intensity images to establish the timing of the emission structures associated with these magnetic structures as a function of height. We found that most of the cancelation sites show either that the bipole is observed longer in the photosphere than in the chromosphere and corona (44%) or that the timing difference of the disappearance of the bipole between these levels of the atmosphere is unresolved. The magnetic axis lengths of the structures associated with the cancelation sites are on average slightly smaller in the chromosphere than the photosphere. These observations suggest that magnetic flux is retracting below the surface for most, if not all, of the cancelation sites studied.  相似文献   

Coming to Terms with “Integrated Coastal Management”: Problems of Meaning and Method in a New Arena of Resource Regulation     

Karen Nichols 《The Professional geographer》1999,51(3):388-399

This paper traces the emergence of coastal management in the late twentieth century and assesses the social and spatial implications of the new Integrated Coastal Management (ICM) philosophy that guides national regulatory programs worldwide. A review of the epistemology of ICM reveals its link to the United Nations marine regulatory regime (the Law of the Sea) and the sustainable development paradigm embraced at the 1992 United Nations Conference on Environment and Development. I suggest that the resulting regulatory regime facilitates the opening of coastal zones worldwide to aggressive state and global capital investment. By promoting the overhaul of existing social and spatial organization in coastal zones and by asserting the primacy of resource access for modern economic interests, ICM may introduce more rather than less social conflict and ecological degradation. To illustrate this dynamic, I examine the case of coral reef management in general, and in the context of the Sri Lankan ICM program.  相似文献   

On the impact of wind on the development of wave field during storm Britta     

Xiaoli Guo Larsén  Jianting Du  Rodolfo Bolaños  Søren Larsen 《Ocean Dynamics》2017,67(11):1407-1427

The observation of extreme waves at FINO 1 during storm Britta on the 1st November 2006 has initiated a series of research studies regarding the mechanisms behind. The roles of stability and the presence of the open cell structures have been previously investigated but not conclusive. To improve our understanding of these processes, which are essential for a good forecast of similarly important events offshore, this study revisits the development of storm Britta using an atmospheric and wave coupled modeling system, wind and wave measurements from ten stations across the North Sea, cloud images and Synthetic Aperture Radar (SAR) data. It is found here that a standard state-of-the-art model is capable of capturing the important characteristics of a major storm like Britta, including the storm path, storm peak wind speed, the open cells, and peak significant wave height (H _s ) for open sea. It was also demonstrated that the impact of the open cells has negligible contribution to the development of extreme H _s observed at FINO 1. At the same time, stability alone is not sufficient in explaining the development of extreme H _s . The controlling conditions for the development of Britta extreme H _s observed at FINO 1 are the persistent strong winds and a long and undisturbed fetch over a long period.  相似文献