Effects of fixation and storage on flow cytometric analysis of marine bacteria     

Eriko Kamiya  Shinji Izumiyama  Masahiko Nishimura  James G. Mitchell  Kazuhiro Kogure 《Journal of Oceanography》2007,63(1):101-112

Flow cytometry (FCM) is now becoming a routine tool for the enumeration and optical characterization of bacteria in marine environments. We investigated the effects of sample fixation and storage upon flow cytometric determination of marine bacteria. Fixed and unfixed seawater samples were analyzed by FCM immediately aboard ship and/or later in the laboratory, and the appearances of the fluorescence signals and bacterial counts of these samples were compared. Fixation and storage led to the formation of multiple peaks in fluorescence histograms; this was also seen in 22 out of 36 samples frozen in liquid nitrogen. Fixation did not, but storage did induce a decrease of bacterial counts: a rapid decrease during the first 3 days followed by a slower decline. The decline of cell numbers in stored samples was expressed by a regression model. Our studies indicate that precaution is necessary when interpreting the data from fixed and/or stored marine bacterial samples analyzed by FCM. The possibility that the procedure of fixation and storage leads to the appearance of high DNA and low DNA bacterial groups should be considered.  相似文献   

Properties of sea ice and overlying snow in the Southern Sea of Okhotsk     

Takenobu Toyota  Shinya Takatsuji  Kazutaka Tateyama  Kazuhiro Naoki  Kay I. Ohshima 《Journal of Oceanography》2007,63(3):393-411

The general properties of sea ice and overlying snow in the southern Sea of Okhotsk were examined during early February of 2003 to 2005 with the P/V “Soya”. Thin section analysis of crystal structure revealed that frazil ice (48% of total core length) was more prevalent than columnar ice (39%) and that stratigraphic layering was prominent with a mean layer thickness of 12 cm, indicating that dynamic processes are essential to ice growth. The mean thickness of ice blocks and visual observations suggest that ridging dominates the deformation process above thicknesses of 30 to 40 cm. As for snow, it was found that faceted crystals and depth hoar are dominant (78%), as which is also common in the Antarctic sea ice, and is indicative of the strong vertical temperature gradients within the snow. Stable isotope measurements (δ¹⁸O) indicate that snow ice occupies 9% of total core length and that the mass fraction of meteoric ice accounts for 1 to 2% of total ice volume, which is lower than the Antarctic sea ice. Associated with this, the effective fractionation coefficient during the freezing of seawater was also derived. Snow ice was characterized by lower density, higher salinity, and nearly twice the gas content of ice of seawater origin. In addition, it is shown that the surface brine volume fraction and freeboard are well correlated with ice thickness, indicating some promise for remote sensing approaches to the estimation of ice thickness.  相似文献   

Discovery of a large-scale clumpy structure around the Lynx supercluster at z∼ 1.27     

Fumiaki Nakata  Tadayuki Kodama  Kazuhiro Shimasaku  Mamoru Doi  Hisanori Furusawa  Masaru Hamabe  Masahiko Kimura  Yutaka Komiyama  Satoshi Miyazaki  Sadanori Okamura  Masami Ouchi †  Maki Sekiguchi  Yoshihiro Ueda  Masafumi Yagi  Naoki Yasuda 《Monthly notices of the Royal Astronomical Society》2005,357(4):1357-1362

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The build-up of the colour–magnitude relation as a function of environment     

Masayuki Tanaka  Tadayuki Kodama  Nobuo Arimoto  Sadanori Okamura  Keiichi Umetsu  Kazuhiro Shimasaku  Ichi Tanaka  Toru Yamada 《Monthly notices of the Royal Astronomical Society》2005,362(1):268-288

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Influence of subduction zone settings on the origin of forearc fluids: Halogen concentrations and I/I ratios in waters from Kyushu,Japan     

Hitoshi Tomaru  Shinji Ohsawa  Kazuhiro Amita  Zunli Lu  Udo Fehn 《Applied Geochemistry》2007

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Stoichiometry among bioactive trace metals in seawater on the Bering Sea shelf   总被引：2，自引：1，他引：1  

Abigail Parcasio Cid  Syouhei Urushihara  Tomoharu Minami  Kazuhiro Norisuye  Yoshiki Sohrin 《Journal of Oceanography》2011,67(6):747-764

The distribution of Al, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb in seawater was investigated on the Bering Sea shelf (56–64°N, 165–169°W) in September 2000. The unfiltered and filtered seawater samples were used for determination of total dissolvable (TD) and dissolved (D) metals (M), respectively. The TD-M concentrations were generally higher than in the Pacific Ocean. TD-Cd was highest in deep water of the outer shelf domain and dominated by dissolved species. The other TD-M were highest at stations close to the Yukon River delta and had higher fractions of labile particulate (LP) species that were obtained as the difference between TD-M and D-M. Dissolved Al, Ni, and Cu were characterized by input from the Yukon River. Dissolved Mn and Co showed maximums on the bottom of the coastal domain, suggesting influence of sedimentary Mn reduction. The correlations of D-Zn, D-Cd, and macronutrients indicated their distributions were largely controlled through uptake by microorganisms and remineralization from settling particles. All these three processes (river input, sedimentary reduction, and biogeochemical cycle) had an influence on the distribution of D-Fe. D-Pb was fairly uniformly distributed in the study area. The stoichiometry of D-M in the Bering Sea shelf showed enrichment of Co and Pb and depletion of Ni, Cu, Zn, and Cd compared with that in the North Pacific. The LP-M/LP-Al ratio revealed significant enrichment of the other eight metals relative to their crustal abundance, suggesting importance of formation of Fe–Mn oxides and adsorption of trace metals on the oxides.  相似文献   

Permian Peri‐glacial Deposits from Central Mongolia in Central Asian Orogenic Belt: A Possible Indicator of the Capitanian Cooling Event     

Tatsuya Fujimoto  Shigeru Otoh  Yuji Orihashi  Takafumi Hirata  Takaomi D. Yokoyama  Masanori Shimojo  Yoshikazu Kouchi  Hokuto Obara  Yasuo Ishizaki  Kazuhiro Tsukada  Toshiyuki Kurihara  Manchuk Nuramkhan  Sersmaa Gonchigdorj 《Resource Geology》2012,62(4):408-422

A dropstone‐bearing, Middle Permian to Early Triassic peri‐glacial sedimentary unit was first discovered from the Khangai–Khentei Belt in Mongolia, Central Asian Orogenic Belt. The unit, Urmegtei Formation, is assumed to cover the early Carboniferous Khangai–Khentei accretionary complex, and is an upward‐fining sequence, consisting of conglomerates, sandstones, and varved sandstone and mudstone beds with granite dropstones in ascending order. The formation was cut by a felsic dike, and was deformed and metamorphosed together with the felsic dike. An undeformed porphyritic granite batholith finally cut all the deformed and metamorphosed rocks. LA‐ICP‐MS, U–Pb zircon dating has revealed the following ²⁰⁶Pb/²³⁸U weighted mean igneous ages: (i) a granite dropstone in the Urmegtei Formation is 273 ± 5 Ma (Kungurian of Early Permian); (ii) the deformed felsic dike is 247 ± 4 Ma (Olenekian of Early Triassic); and (iii) the undeformed granite batholith is 218 ± 9 Ma (Carnian of Late Triassic). From these data, the age of sedimentation of the Urmegtei Formation is constrained between the Kungurian and the Olenekian (273–247 Ma), and the age of deformation and metamorphism is constrained between the Olenekian and the Carnian (247–218 Ma). In Permian and Triassic times, the global climate was in a warming trend from the Serpukhovian (early Late Carboniferous) to the Kungurian long and severe cool mode (328–271 Ma) to the Roadian to Bajocian (Middle Jurassic) warm mode (271–168 Ma), with an interruption with the Capitanian Kamura cooling event (266–260 Ma). The dropstone‐bearing strata of the Urmegtei Formation, together with the glacier‐related deposits in the Verkhoyansk, Kolyma, and Omolon areas of northeastern Siberia (said to be of Middle to Late Permian age), must be products of the Capitanian cooling event. Although further study is needed, the dropstone‐bearing strata we found can be explained in two ways: (i) the Urmegtei Formation is an autochthonous formation indicating a short‐term expansion of land glacier to the central part of Siberia in Capitanian age; or (ii) the Urmegtei Formation was deposited in or around a limited ice‐covered continent in northeast Siberia in the Capitanian and was displaced to the present position by the Carnian.  相似文献   

Properties of winter mixed layer variability on the shelf-slope region facing the Kuroshio—study of Tosa Bay, southern Japan     

Hiroshi Kuroda  Yuichi Hirota  Takashi Setou  Kazuhiro Aoki  Daisuke Takahashi  Tomowo Watanabe 《Ocean Dynamics》2014,64(1):47-60

To examine the properties of winter mixed layer (ML) variability in the shelf-slope waters facing the Kuroshio, we analyzed historical temperature records and the simulated results of a triply nested high-resolution numerical model. As a candidate of the shelf-slope waters, we focused on Tosa Bay, off the southern Japan. A time series of observed monthly mean ML temperatures and depths in the bay exhibits a remarkable seasonal variation. The period when the ML develops can be divided into two regimes: from September to November, when the sea surface cooling is gradually enhanced, the ML temperature and depth decreases and increases, respectively; from January to March, the ML temperature and depth are kept nearly constant, while the sea surface cooling in January reaches its annual maximum. In the latter regime, variance for the monthly mean ML depth is the largest of the year. To further study the ML properties in the latter regime corresponding to winter, we examined simulated results. It was found that the largest variance for ML depth is attributed to a dominant intramonthly variation. This is related to a submesoscale variation with typical spatial scales of 10–20 km, induced by the Kuroshio and its frontal disturbances. Simulated monthly mean heat balance within the ML showed that heat advection balances with heat flux at the sea surface and entrainment through the ML bottom. Moreover, the monthly mean heat advection is determined mainly by the intramonthly eddy heat advection, suggesting that the high-frequency intramonthly variation related to submesoscale variations contributes significantly to the low-frequency monthly variations of the ML in winter.  相似文献   

Dissolved iron and zinc in Sagami Bay and the Izu-Ogasawara Trench     

Hajime Obata  Jun Nishioka  Taejin Kim  Kazuhiro Norisuye  Shigenobu Takeda  Yohei Wakuta  Toshitaka Gamo 《Journal of Oceanography》2017,73(3):333-344

Using a clean seawater sampling system for trace metals onboard the R. V. Shinsei-Maru, newly launched in 2013, we investigated the vertical distributions of dissolved iron and zinc in Sagami Bay and the Izu-Ogasawara Trench. We applied appropriate clean sampling and filtering processes for trace metals, so that uncontaminated seawater samples were successfully collected. The distribution of zinc in the trench area was similar to that of silicate and the same as that previously reported in the subtropical North Pacific. There were spatial variations in the iron (Fe) distribution in the trench areas. We used previously reported information about biogeochemical cycling in the trench area, and found that Fe has a residence time of 29 years in the water column. The short residence time of Fe (29 years) corresponds to the vertical variations of dissolved Fe in the water column.  相似文献   

Spatial and temporal distribution of Fe, Ni, Cu and Pb along 140°E in the Southern Ocean during austral summer 2001/02     

Xiaodong Lai  Kazuhiro Norisuye  Michi Mikata  Tomoharu Minami  Andrew R. Bowie  Yoshiki Sohrin   《Marine Chemistry》2008,111(3-4):171-183

The distribution of dissolved (D) and acid-dissolvable (AD) Fe, Ni, Cu and Pb in the upper water column (0–300 m depth) was determined in the Australian sector of the Southern Ocean (140°E meridian) during three cruises conducted between November 2001 and March 2002. For Ni and Cu, there was no significant difference in concentration between dissolved and acid-dissolvable species. DNi and DCu showed significant (P = 0.01) positive correlations with silicate, phosphate and nitrate, reflecting their strong nutrient-type behaviour. For Fe and Pb, the acid-dissolvable concentration mostly exceeded the dissolved concentration, reflecting the importance of labile particulate species for these elements. DPb decreased between January and February in the Polar Frontal Zone and in Antarctic continental shelf water. ADPb maxima occurred in the Antarctic Zone, resulting in a maximum AD/D ratio of 7. The mean DFe concentration in the surface mixed layer was 0.3 nM in the sub-Antarctic zone, 0.4 nM in the Polar Frontal Zone, 0.5 nM in the Antarctic Zone and increased southward beyond the Antarctic Divergence and towards the continent. DFe did not show a clear temporal change in its horizontal distribution, which was in contrast to the other nutrients and trace metals. ADFe substantially increased in Antarctic continental shelf water where the AD/D ratio reached 11. The following conclusions can be drawn from these data. (1) Ni and Cu exist exclusively as dissolved species and their distributions are mainly controlled by their biogeochemical cycling, similar to those of the major nutrients. (2) Pb is dominated by particulate species. The distribution of DPb is temporally and spatially variable due to a sporadic source and strong scavenging. (3) DFe is rather a minor fraction of total Fe in Antarctic continental shelf water where shelf sediments and Antarctic sea-ice appear to be strong sources for Fe. There is substantial temporal variation in the supply of Fe to the upper water column. DFe in the mixed layer of the open Southern Ocean is maintained at low concentrations throughout summer due to uptake by phytoplankton and scavenging.  相似文献