Effects of dithiocarbamate and 8-hydroxyquinoline additions on algal uptake of ambient copper and nickel in South San Francisco Bay water     

Jonathan T. Phinney  Kenneth W. Bruland 《Estuaries and Coasts》1997,20(1):66-76

The addition of synthetic organic ligands such as diethyldithiocarbamate (DDC) and 8-hydroxyquinoline (Ox) to the dissolved fraction (<0.2 μm) of South San Francisco Bay water facilitated the transport of ambient Cu and Ni into phytoplankton cells. The uptake mechanism is diffusion of the lipophilic organic Cu(DDC)₂ ⁰ and Cu(Ox)₂ ⁰ complexes (and corresponding Ni complexes) across the plasma membrane. Short-term uptake experiments were carried out using a coastal diatom,Thalassiosira weissflogii, and resulted in rapid uptake rates and high cellular concentrations of the metal relative to the bay water control. Steady-state conditions between the solution and cellular Cu concentrations occurred within 10 min for a 4 μM addition of DDC and after 3 h for a 100 μM addition of Ox. Steady-state cellular Cu concentrations were over 10 times and 6 times greater, for DDC and Ox treatments, than in the bay water controls. Steady-state cellular Ni concentrations were attained within 10 min for both ligand additions and were more than 6 times and 2 times greater than in the absence of the added ligands. Using this bioassay, we were also able to gain insight into the character of the background organic Cu complexes in South Bay. Our results suggest that the natural organic Cu complexes are relatively hydrophilic in character and do not appear to be directly assimilated across the plasmalemma.  相似文献   

Extreme ultraviolet spectra of solar active regions and their analysis     

Kenneth P. Dere 《Solar physics》1982,77(1-2):77-93

Extreme ultraviolet spectra of several active regions are presented and analyzed. Spectral intensities of 3 active regions observed with the NRL Skylab XUV spectroheliograph (170–630 Å) are derived. From this data density sensitive line ratios of Mg viii, Si x, S xii, Fe ix, Fe x, Fe xi, Fe xii, Fe xiii, Fe xiv, and Fe xv are examined and typically yield, to within a factor of 2, electron pressures of 1 dyne cm^–2 (n _e T = 6 × 10¹⁵ cm^–3 K). The differential emission measure of the brightest 35 × 35 portion of an active region is obtained between 1.4 × 10⁴ K and 5 × 10⁶ K from HCO OSO-VI XUV (280–1370 Å) spectra published by Dupree et al. (1973). Stigmatic EUV spectra (1170–1710 Å) obtained by the NRL High Resolution Telescope and Spectrograph (HRTS) are also presented. Doppler velocities as a function of position along the slit are derived in an active region plage and sunspot. The velocities are based on an absolute wavelength scale derived from neutral chromospheric lines and are accurate to ±2 km s^–1. Downflows at 10⁵ K are found throughout the plage with typical velocities of 10 km s^–1. In the sunspot, downflows are typically 5 to 20 km s^–1 over the umbra and zero over the penumbra. In addition localized 90 and 150 km s^–1 downflows are found in the umbra in the same 1 × 1 resolution elements which contain the lower velocity downflows. Spectral intensities and velocities in a typical plage 1 resolution element are derived. The velocities are greatest ( 10 km s^–1) at 10⁵ K with lower velocities at higher and lower temperatures. The differential emission measure between 1.3 × 10⁴ K and 2 × 10⁶ K is derived and is found to be comparable to that derived from the OSO-VI data. An electron pressure of 1.4 dynes cm^–2 (n _e T = 1.0 × 10¹⁶ cm^–3 K) is determined from pressure sensitive line ratios of Si iii, O iv, and N iv. From the data presented it is shown that convection plays a major role in determining the structure and dynamics of the active region transition zone and corona.  相似文献   

Chemical methods of silicate analysis: H. Bennett and R.A. Read. Academic Press,London and New York, 1971, 272 pp., £ 4.00     

A.S. Ritchie 《Chemical Geology》1973,11(3):231-232

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A single loop of 21 January 1974 flare     

Eijiro Hiei  Kenneth G. Widing 《Solar physics》1979,61(2):407-417

A single loop associated with a flare of 21 January 1974 was studied with NRL spectroheliograms in order to understand the phenomenon of evaporation. The loop seen in the emission lines of Fe xv reached its maximum brightness 15 min after the onset. The loop is different from a flare loop because of the time sequence in which it appeared and is different from a post-flare loop prominence system because of its morphology. The electron density in the loop increases gradually to 4 × 10¹⁰ cm^–3. The material of the loop is thought to be supplied from the lower atmosphere of the chromosphere or the photosphere. The loop is an associated phenomenon of the main flare event distinguished by a longer rise time (15 min) and a lower peak temperature (2 × 10⁶ K).  相似文献   

Sources and sinks of low molecular weight organic carbonyl compounds in seawater     

Kenneth Mopper  William L Stahovec 《Marine Chemistry》1986,19(4)

Results from laboratory studies indicated that low molecular weight (LMW) carbonyl compounds, especially formaldehyde, acetaldehyde, acetone and glyoxal, can be formed in seawater by photochemical processes. Once formed, these compounds appear to be readily consumed by biota. These results suggest that concentrations of LMW carbonyl compounds should undergo diurnal variations in the illuminated layer of the sea. In support of this, diurnal fluctuations of LMW carbonyl concentrations were observed in humic-rich surface waters off the west coast of Florida over a three day sampling period using a shipboard HPLC system. Fluctuations in acetaldehyde were particularly strong and reproducible, with steady night-time concentrations of 2–3 nM and day-time concentrations reaching a maximum of 20–30 nM in the early afternoon. In contrast, diurnal fluctuations in formaldehyde were less distinct, ranging from 15 to 50 nM.The laboratory and field results are discussed in terms of biotic/abiotic sources and sinks of LMW carbonyl compounds in surface seawater. It is speculated that photooxidative cleavage of biologically refractory dissolved organic matter (DOM) in seawater to yield LMW organic fragments, such as carbonyl compounds, may be important in the breakdown and geochemical cycling of DOM in the ocean.  相似文献   

Fish assemblages across the marine to low salinity transition zone of a temperate estuary   总被引：2，自引：0，他引：2  

Edward J. Martino  Kenneth W. Able 《Estuarine, Coastal and Shelf Science》2003,56(5-6):969-987

This 3-year study provides a large-scale perspective of fish assemblage structure across an ocean–estuarine ecotone, given range of salinity encountered (0.1–32) based on sampling at 12 stations along 40 km from the Mullica River (river), Great Bay (bay), and the adjacent inner continental shelf (ocean) in southern New Jersey. Otter trawl (4.9 m, 6 mm mesh) collections were dominated by young-of-the-year of most of the 49 species encountered. Species richness and abundance appeared greatest in the ocean, decreased (with an increase in inter-station variability) in the bay, and appeared to increase again towards the uppermost river stations. The same areas contained three non-discrete, but identifiable, fish assemblages based on Detrended Correspondence Analysis. Members of the Triglidae and Stromateidae characterized the ocean and bay, whereas representatives of the Percichthyidae and Ictaluridae characterized the river. Several species, including Anchoa mitchilli and Cynoscion regalis, exhibited a ubiquitous distribution across the sampling area. Further analyses with Canonical Correspondence Analysis identified salinity and geographic distance, among the variables examined, as the most important determinants in shaping the assemblages. Other contributors included habitat heterogeneity and water depth. In summary, these observations indicate that large-scale patterns in the structure of this estuarine fish assemblage are primarily a result of individual species' responses to dominate environmental gradients, as well as ontogenetic migrations, whereas smaller-scale patterns appear to be the result of habitat associations that are most likely driven by foraging, competition, and/or predator avoidance.  相似文献   

Reduction of iodate in seawater during Arabian Sea shipboard incubations and in laboratory cultures of the marine bacterium Shewanella putrefaciens strain MR-4     

Anna M. Farrenkopf  Michael E. Dollhopf  Sinad Ní Chadhain  George W. Luther  III  Kenneth H. Nealson 《Marine Chemistry》1997,57(3-4)

Shipboard incubations from the US JGOFS cruise to the Arabian Sea (TN045) March, 1995 showed evidence of iodate reduction in 0.45 μ (Gelman Supor membrane) filtered seawater samples collected from intermediate depths (200–600 m) within the oxygen minimum zone (OMZ). Inorganic chemical reduction of iodate in these samples was ruled out as no free sulfide was measurable and concentrations of ammonia and nitrite were found to be less than 5 μM. To examine whether the reduction of iodate observed at sea could have been the result of bacterial metabolism, reduction of iodate (IO₃⁻) to iodide (I⁻) by Shewanella putrefaciens strain MR-4 was studied in artificial seawater using electrochemical methods. MR-4 is a ubiquitous marine bacterium which may be of considerable importance when considering redox zonation in the water column because it is a facultative anaerobe and may switch amongst a suite of electron acceptors to support metabolism. In all experiments MR-4 reduced all iodate to iodide. The rate of formation of [I⁻]in the culture followed pseudo-first order kinetics. This is the first report of the marine bacterial reduction of iodate where the concentrations of iodide and iodate were measured directly. Our results may help to explain the depth distribution of iodine speciation reported in productive waters like the Arabian Sea and for the first time couple iodine speciation with bacterial productivity in the ocean.  相似文献   

Phytoplankton growth response to deep ocean water     

Kenneth L. Terry  John Caperon 《Marine environmental research》1982,7(3):211-225

Single species populations and natural populations of phytoplankton were grown in mixtures of surface sea water and deep ocean water. The yields of Phaeodactylum tricornutum and the natural species assemblages were positively correlated with the percentage of deep water in the mixture. Single species populations of Thalassiosira weissflogii and Amphidinum sp. showed yields which were positively correlated with per cent deep water at low deep water concentrations and negatively correlated at high deep water concentrations. Transition from the positive to the negative slope occurred at about 75% deep water for T. weissflogii and at about 25% deep water for Amphidinium. Populations were apparently limited by an inorganic nutrient, probably nitrogen, in the region of positive slope and by some other factor, probably organic, in the region of negative slope. The addition of EDTA increased the yields of T. weissflogii and Amphidinium in the presence of deep water and reduced lag times in the growth of the natural populations.  相似文献   

Contemporary management issues confronting fisheries science     

Kenneth T. Frank  David Brickman 《Journal of Sea Research》2001,45(3-4)

Stock collapses have occurred worldwide. The most frequently cited cause is over-fishing, suggesting that fisheries management has been ineffective in controlling exploitation rates. The progression of a fishery from an over-exploited to a collapsed state involves impairment of the reproductive capacity of the target species, i.e. recruitment over-fishing. In many cases, this occurs by reduction of the spawning stock biomass (SSB) through the systematic elimination of spawning components within a stock complex. While operational definitions of minimum levels of SSB have been developed, they have seldom been applied and never adopted in a Canadian groundfish management context. The answer to the question of how much is enough to perpetuate a stock under exploitation has been illusive. Serebryakov [J. Cons. Int. Explor. Mer, 47 (1990) 267] has advocated definition of critical levels of SSB based on survival rates (R/SSB). We review his method and discuss the utility of the approach. An alternative approach to the problem of estimating minimum SSB is through a fundamental revision of the traditional stock and recruitment relationship. Explicit theoretical SSB thresholds below which reproduction/recruitment is severely impaired based upon density-dependent mating success (or Allee effects) is considered a superior approach to the question of how much is enough because of its ecological grounding. However, the successful application of this approach will require re-definition of the space/time scales of the management unit. Finally, support is growing for the establishment of closed areas or “no-take zones” as an alternative approach to managing the problems of fishing a stock complex by enabling sub-populations to escape fishing. While the expected benefits of areas protected from fishing are numerous, clear demonstrations of benefits of such areas in marine temperate ecosystems are lacking. In fact, unintended negative consequences may result from such actions.  相似文献   

Doubling the production and precision of the MBTH spectrophotometric assay for dissolved carbohydrates in seawater     

Kenneth M. Johnson  Curtis M. Burney  John McN. Sieburth 《Marine Chemistry》1981,10(6):467-473

Extensive use of the three step 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) method for determining concentrations of both free and combined dissolved carbohydrate occurring in diverse marine environments has shown its suitability for studies of carbohydrate dynamics. Modification of the analytical sequence to extend over 2 working days to eliminate a confusing and error producing overlap of analytical operations allows a single analyst to determine 15 instead of 8 samples in the same 2-day period. This modification also permits an increase in the number of blank plus replicate determinations from 5 to 7 per sample so that a total of 105 reaction tubes can be assayed every 2 days compared to 40–50 for the original procedure. For a series of 7 Narragansett Bay samples, the standard deviation of the method serendipitously decreased by 50% (from ±5.3 to ±2.7 μg C l⁻¹) when the original procedure was compared with the modified procedure. Postponing the final analytical step for a day when the analyses can be completed with an autoprocessor will free the analyst for the processing of more samples, causing an additional doubling in productivity.  相似文献