固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

Glaciomarine sedimentation and palaeo-glacial setting of Maxwell Bay and its tributary embayment, Marian Cove, South Shetland Islands, West Antarctica     

Ho Il Yoon  Myung Woo Han  Byong-Kwon Park  Jae-Kyung Oh  Soon-Keun Chang 《Marine Geology》1997,140(3-4):265-282

High-resolution (3.5 kHz and multi-channel) seismic profiles and piston cores were collected from Maxwell Bay and its tributary embayment, Marian Cove, in the South Shetland Islands, Antarctica, during the Korea Antarctic Research Program (1992/93 and 1995/96) to elucidate the glaciomarine sedimentation processes and recent glacial history of the area. Seismic data from Maxwell Bay reveal a rugged bay margin and flattened basin floor covered with well-stratified hemipelagic muds. On the base-of-slope, acoustically transparent debris flows occur, indicating downslope resedimentation of glaciomarine sediments. Despite the subpolar and ice-proximal settings of Marian Cove, the seafloor is highly rugged with a thin sediment drape, suggesting that much of the area has been recently eroded by glaciers. Sediment cores from the cove penetrated three distinct fining-upward lithofacies: (1) basal till in the lower part of the core, accumulated just seaward of the grounding line of the tidewater glacier; (2) interlaminated sand and mud in the middle part, deposited in ice-proximal zone by a combination of episodic subglacial meltwater inflow and iceberg dumping; and (3) pebbly mud in the upper part, deposited in ice-distal zone by both surface meltwater plume and ice-rafting from the glacier front. A reconstruction of the glacial history of these areas since the late glacial maximum shows an ice sheet filling Maxwell Bay in late Wisconsin time and grounding of the tidewater glacier in Marian Cove until about 1300 yr BP.  相似文献   

养殖对虾病毒性疾病的细菌并发症防治研究   总被引：1，自引：0，他引：1  

苏国成  陈金翠 《台湾海峡》1996,15(2):200-204

本文研究报道了发病对虾肝胰腺分离菌物敏感性，采用防台细菌人工感染方法进行药物饲料的筛选，在实验室及养殖池中进行药物饲料的防治效果试验。结果表明，在对虾病毒流行期间，采用药物饲料可有效防止对虾爆发性大量死亡，提高对虾存活率。  相似文献   

Forearc structures and tectonics in the southern Peru—northern Chile Continental Margin     

Chang Li 《Marine Geophysical Researches》1995,17(1):97-113

SeaMARC II side-scan images, bathymetry, and single-channel seismic reflection data along the southern Peru—northern Chile forearc area between 16° and 23° S reveal a complex region of morpho-structural, submarine drainage and depression patterns. In the subducting plate area, the NW—SE trending primary normal fault system represented by trench-paralleled scarps was incipiently formed as the Nazca Plate was bent in the outer edge and further intensified as the plate approached the trench. The NE—SW trending secondary normal fault system that consists of discontinuous and smaller faults, usually intersect the primary trench-paralleled fault system. Similar to the Nazca Plate, the overriding continental plate also shows two major NW—SE and NE—SW trending fault systems represented by fault scarps or narrow elongated depressions.The submarine drainage systems represented by a series of canyon and channel courses appear to be partly controlled by the faults and exhibit a pattern similar to the onshore drainage which flows into the central region of the coastal area. Two large depressions occurring along the middle—upper slope areas of the continental margin are recognized as collapse and slump that perhaps are a major result of increased slope gradient. The subsidence of the forearc area in the southern Peru—northern Chile Continental Margin is indicated by: a) drainage systems flowing into the central region, b) the slope collapse and slumps heading to the central region, c) the deepening of the trench and inclining of the lower slope terrace to the central region, and d) submerging of the upper-slope ridge and the Peru—Chile Coast Range off the Arica Bight area.The subsidence of the forearc area in the southern Perunorthern Chile margin is probably attributed to a subduction erosion which causes wearing away and removal of the rock and sedimentary masses of the overriding plate as the Nazca Plate subducts under the South American Plate.  相似文献   

Reanalysis of the Atmospheric Flux of Nutrient Elements to the Southern Yellow Sea and the East China Sea     

万小芳吴增茂  常志清 《海洋通报(英文版)》2003,5(1):45-51

Based on the recent research results on dry and wet deposition of nutrient elements and sulphate,we estimate the atmospheric flux of nutrient elements and sulphate to the southern Yellow Sea and the East China Sea in each season.The results suggest that the concentrations of nutrient elements and sulphate in aerosol and precipitation show an apparent seasonal cycle with the maximum values in winter and the minimum values in summer.Depositions of nitrate and sulphate are dominated by wet deposition,while the deposition for phosphate is mainly dry deposition.Moreover,compared with the riverine inputs,the atmospheric deposition may be the main source of dissolved inorganic nutrients in the southern Yellow Sea and the East China Sea.  相似文献   

双因素判别分析胃癌组织与正常组织自体荧光光谱     

马君  史晓凤  李颖  王荣  毛伟征  郑荣儿  孟继武 《中国海洋大学学报(自然科学版)》2004,34(4):662-666

通过对手术切除的37例胃癌患者的离体标本的自体荧光光谱检测,显示308nm,337nm激发光谱均呈双主峰结构,双主峰的比值均能区分胃癌与正常胃壁(P<0.001).分别用308nm,337nm激发荧光光谱单因素分析诊断胃癌的灵敏度、特异度、阳性预测值分别为88.8%,86.8%,86.4%和80.1%,72.1%,67.5%.对308nm及337nm激发荧光光谱双因素分析,癌诊断的灵敏度、特异度、阳性预测值分别为94.4%,92.1%,91.9%,其诊断正确符合率明显高于单因素分析的结果.研究表明,以308nm及337nm双波长激发检测胃壁浆膜,双因素判别算法可较好地区分胃癌,这种方法有望成为手术中快速识别胃癌在胃壁的浸润范围的有效方法.  相似文献   

南海东北部表层沉积中生源和矿物碎屑组分分析及其古环境意义   总被引：9，自引：3，他引：9  

孟翊  严肃庄  陈荣华  张富元 《海洋地质与第四纪地质》2001,21(3):17-22

通过对南海东北部128个表层沉积样品的定量研究，揭示隆源和矿物碎屑各组分的分布，也显示了组分分析作为一种简单、快速的研究方法在海洋古环境研究中的潜力，研究区生源碎屑的分布明显受与水深相关的深海溶解作用所控制，水深3500-4000m的现代南海CCD以下，钙质生物的含量急剧减少，而硅质生物的含量急剧增加，研究区表层沉积中的碎屑矿物主要来源于亚洲大陆，吕宋镐的风化产物只起次要作用，且其分布主要受与离岸距离远近和海流相关的搬运作用所控制。  相似文献   

青岛海陆风三维结构的数值模拟   总被引：4，自引：1，他引：4  

常志清  吴增茂  高山红 《中国海洋大学学报(自然科学版)》2002,32(6):877-883

本文采用 1个陡地形影响修正的三维中尺度流体静力的气象学模式 ,对青岛地区海陆风的日变化规律和三维结构进行了较细致的分析。结果显示 ,青岛有多支海陆风存在 ,且每支海陆风出现的时间、强度和向内陆伸展的距离有很大的不同。这其中沿岸山地的机械和热力作用扮演着重要的角色。在观测站 ,模拟结果和实测资料等方面有较好的一致性。  相似文献   

是彗星导致的大洪水吗     

Scott Carney  刘畅 《海洋地质前沿》2007,23(12):26-27

一条巨蛇的多条尾巴令人恐怖地缠绕在一起,一只尖角从头顶伸出.这个怪物似乎正在遨游星球的海洋,或它正在攀爬一个陡峭的玄武岩悬崖?美国洛斯阿拉莫国家实验室的环境考古学家Bruce Masse 看到这个古代古刻,坚定地认为,这是一颗慧明.……  相似文献   

Pb²⁺对青蛤的急性毒性及对其血淋巴液中免疫相关酶活性的影响     

王瑞  黄雨辰  熊德东  黄亚  田甜  孟彬  梁健 《海洋科学》2020,44(3):59-65

采用静态急性毒性实验研究了重金属Pb~(2+)对青蛤(Cyclina sinensis)的生物急性毒性效应,测定了在96 h Pb~(2+)半致死浓度的1/10(TC组)和1/100(SC组)两个浓度胁迫下,血淋巴液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LSZ)活性的变化。结果显示:Pb~(2+)的96 h LC50为7.938 mg/L; SC组ACP活性表现为诱导-抑制趋势,除4 d外均与对照组差异显著(P0.05), TC组为抑制趋势,为3个组中的最低,与对照组差异显著(P0.05);实验组SC组和TC组的AKP、LSZ活性均表现为前期为诱导中后期受抑制的趋势,与对照组差异显著(P0.05),且TC组活性始终低于SC组,表现出Pb~(2+)的胁迫浓度越高酶活性受到的抑制作用越大。以上结果表明,重金属Pb~(2+)对青蛤的毒性级别为高毒级,能造成青蛤免疫相关酶的活性受到抑制,影响青蛤的免疫能力,而且这种抑制作用随着环境中Pb~(2+)浓度增加而增加。  相似文献