Integrated use of biomarkers (acetylcholinesterase and antioxidant enzymes activities) in Mytilus galloprovincialis and Mullus barbatus in an Italian coastal marine area   总被引：1，自引：0，他引：1  

Lionetto MG  Caricato R  Giordano ME  Pascariello MF  Marinosci L  Schettino T 《Marine pollution bulletin》2003,46(3):324-330

The use of biomarkers to evaluate the biological effects of chemical pollutants in marine organisms represents a recent tool in the monitoring field responding to the need to detect and assess the effects of chemical contaminants on the biota. The aim of the present work was the field application of the integrated use of acetylcholinesterase (AChE) and antioxidant enzymes (catalase--CAT, glutathione peroxidase--GSH-Px), for detecting the possible exposure/effect induced by chemical pollutants in native marine organisms from a coastal marine area, represented by Salento Peninsula (Italy), that shows a coastline of high environmental value, but under constant urban pressure, including agriculture activities, widely diffused in the whole hinterland. Eight sampling stations were chosen: four not urbanized areas considered "uncontaminated" controls and four clearly exposed to anthropogenic impact. The bioindicator species studied were a sessile invertebrate, Mytilus galloprovincialis, and a benthic teleost fish, Mullus barbatus.AChE activity in M. galloprovincialis revealed significant differences among places; the minimum values observed (3.9+/-1.8 nmolmin(-1)mg(-1)) was about 50% reduced with respect to the maximum found (11.4+/-0.9 nmolmin(-1)mg(-1)). The reduction in AChE activity observed in two control stations could be explained by the leaching of pesticides into the sea from the agricultural lands. Moreover, the inhibition of AChE activity by heavy metals besides pesticides, can also explain the reduction of the enzymatic activity observed in an industrialized and harbour area. In M. galloprovincialis AChE activity showed a significant inverse correlation with catalase activity but not with glutathione peroxidase that did not significantly change in animals sampled from the eight stations. Also in M. barbatus AChE activity showed significant differences among places; it was inversely correlated with liver GSH-Px activity, but not with catalase activity, that did not show any significantly variation in animals sampled in the different stations.In conclusion, the integrated use of AChE and antioxidant enzymes (catalase or glutathione peroxidase) in M. galloprovincialis and M. barbatus, two species living in different compartment of marine coastal ecosystem, can find a useful application within the framework of marine coastal environment monitoring programs for detecting the possible exposure/effect induced by chemical pollutants, including pesticides, on living marine organisms.  相似文献   

东北——内蒙古地区矿山环境灾害和防治类型的研究     

曹玉清  金永铢 《华北地质矿产杂志》1997,12(3):293-297

东北—内蒙古地区矿山环境灾害和防治类型的研究曹玉清胡宽金永铢（长春科技大学）（东北－内蒙古煤炭工业联合公司生产部）我国东北、内蒙古地区侏罗系、第三系赋存有强度不同的厚煤、特厚煤层。围岩是强度低的泥岩和流沙。开采煤层时，不仅采场顶板岩石破裂具有其独特...  相似文献   

Hepatic ethoxyresorufin O-deethylase (EROD) activity in flounder (Platichthys flesus) from contaminant impacted estuaries of the United Kingdom: continued monitoring 1999-2001     

Kirby MF  Neall P  Bateman TA  Thain JE 《Marine pollution bulletin》2004,49(1-2):71-78

The determination of hepatic ethoxyresorufin O-deethylase (EROD) has been used to assess the induction of the mixed function oxygenase system (MFO) of flounder (Platichthys flesus) in UK estuaries. Induction of the MFO system denotes possible exposure to certain organic contaminants (e.g. polycyclic aromatic hydrocarbons, polychlorinated biphenyls) and its measure has been incorporated in national monitoring programmes. This study presents EROD monitoring data from 5 UK estuaries taken between 1999 and 2001 and builds on data from previous years. The results reveal that for all sampled estuaries EROD values have been significantly (p < 0.05) elevated on the majority of occasions in comparison with the reference estuary, the Alde in Suffolk, UK. However, the limited temporal scale of the reported monitoring does not allow any conclusions to be drawn with respect to trends in the data. Possible factors influencing the data (size, gender, seasonality, reproductive status, etc.) are discussed and recommendations for continued monitoring are made.  相似文献   

封闭岩溶泉域水化学场及其与岩溶发育的关系——以峰峰黑龙洞泉域为例     

曹玉清  胡宽瑢 《水文地质工程地质》1984,(4)

在基本封闭的岩溶泉域内,岩溶发育的水平与垂直分布和变化规律,除研究区内地质构造和结构,碳酸盐矿物及化学成分,泉域水动力条件等外,还应研究水化学场及其对岩溶形成和改造的作用;这就涉及到碳酸盐岩溶解与沉淀的物理化学平衡理论问题。这些理论在国外岩溶研究上已广为运用,但国内结合地区的资料还比较少。我们用该理论研究了几个泉域的水化学场,基本上可以概括成两样计算模式,并取得了与实际相吻合的结果。下面以峰峰黑龙洞泉域为例  相似文献