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Genomic data can be a useful tool in the management and conservation of biodiversity. Here, we report the development of genomic resources for the spotted ragged-tooth shark Carcharias taurus using genome-wide DNA data from Illumina next-generation sequencing. We explored two commonly used genetic marker types: microsatellites and mitochondrial DNA. A total of 4 394 putative microsatellites were identified, of which 10 were tested on 24 individuals and found to have ideal properties for population genetic analyses. Additionally, we reconstructed the first complete mitochondrial genome of a South African spotted ragged-tooth shark, and highlight the most informative gene regions to facilitate future primer design. The data reported here may serve as a resource for future studies and can ultimately be applied in the sustainable conservation and fisheries management of this apex predator.  相似文献   
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This paper reports a series of laboratory and field measurements of spectral reflectance under artificial and natural light conditions which demonstrate that effects of natural chlorophyll fluorescence are observable in the reflectance red edge spectral region. These are results from the progress made to link physiologically-based indicators to optical indices from hyperspectral remote sensing in the Bioindicators of Forest Sustainability Project. This study is carried out on twelve sites of Acer saccharum M. in the Algoma Region, Ontario (Canada), where field measurements, laboratory-simulation experiments, and hyperspectral CASI imagery have been carried out in 1997, 1998, 1999 and 2000 campaigns. Leaf samples from the study sites have been used for reflectance and transmittance measurements with the Li-Cor Model 1800 integrating sphere apparatus coupled to an Ocean Optics Model ST1000 fibre spectrometer in which the same leaves are illuminated alternatively with and without fluorescence-exciting radiation. A study of the diurnal change in leaf reflectance spectra, combined with fluorescence measurements with the PAM-2000 Fluorometer show that the difference spectra are consistent with observed diurnal changes in steady-state fluorescence. Small canopies of Acer saccharum M. have been used for laboratory measurements with the CASI hyperspectral sensor, and under natural light conditions with a fibre spectrometer in diurnal trials, in which the variation of measured reflectance is shown experimentally to be consistent with a fluorescence signature imposed on the inherent leaf reflectance signature. Such reflectance changes due to CF are measurable under natural illumination conditions, although airborne experiments with the CASI hyperspectral sensor produced promising but less convincing results in two diurnal experiments carried out in 1999 and 2000, where small variations of reflectance due to the effect of CF were observed.  相似文献   
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The partitioning of rainfall into surface runoff and infiltration influences many other aspects of the hydrologic cycle including evapotranspiration, deep drainage and soil moisture. This partitioning is an instantaneous non-linear process that is strongly dependent on rainfall rate, soil moisture and soil hydraulic properties. Though all rainfall datasets involve some degree of spatial or temporal averaging, it is not understood how this averaging affects simulated partitioning and the land surface water balance across a wide range of soil and climate types. We used a one-dimensional physics-based model of the near-surface unsaturated zone to compare the effects of different rainfall discretization (5-min point-scale; hourly point-scale; hourly 0.125° gridded) on the simulated partitioning of rainfall for many locations across the United States. Coarser temporal resolution rainfall data underpredicted seasonal surface runoff for all soil types except those with very high infiltration capacities (i.e., sand, loamy sand). Soils with intermediate infiltration capacities (i.e., loam, sandy loam) were the most affected, with less than half of the expected surface runoff produced in most soil types when the gridded rainfall dataset was used as input. The impact of averaging on the water balance was less extreme but non-negligible, with the hourly point-scale predictions exhibiting median evapotranspiration, drainage and soil moisture values within 10% of those predicted using the higher resolution 5-min rainfall. Water balance impacts were greater using the gridded hourly dataset, with average underpredictions of ET up to 27% in fine-grained soils. The results suggest that “hyperresolution” modelling at continental to global scales may produce inaccurate predictions if there is not parallel effort to produce higher resolution precipitation inputs or sub-grid precipitation parameterizations.  相似文献   
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In this study, the Land Use Dynamic Simulator model was applied to investigate the impact of farm credit as an adaptation strategy to cope with effects of climate variability on agricultural land‐use change and crop production in the Vea watershed in Ghana. The authors identified the determinants of crop choices within the landscape (e.g., farm household and biophysical characteristics of farm plot). The crop choice sub‐model was then linked to the crop yield sub‐model to determine the yields of selected crops. In adapting to the impacts of climate variability, the maize credit adoption sub‐model under the maize cultivation credit scenario was integrated into decision‐making. This was simulated for a 20‐year period, and compared with the business‐as‐usual scenario. Under the simulated maize credit scenario, maize adopters increased from about 20 per cent to about 50 per cent and the area allocated for maize cultivation significantly increased by about 266 per cent. Consequently, the average annual aggregated household crop yield increased by 6.3 per cent higher than in the business‐as‐usual scenario. This simulation study shows that access to maize credit can significantly influence agricultural land‐use change and food availability in the study area. However, although access to farm credit may translate into food availability, the sustainability of this strategy is questionable.  相似文献   
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Recent high profile flood events have highlighted the need for hydraulic models capable of simulating pluvial flooding in urban areas. This paper presents a constant velocity rainfall routing scheme that provides this ability within the LISFLOOD‐FP hydraulic modelling code. The scheme operates in place of the shallow water equations within cells where the water depth is below a user‐defined threshold, enabling rainfall‐derived water to be moved from elevated features such as buildings or curbstones without causing instabilities in the solution whilst also yielding a reduction in the overall computational cost of the simulation. Benchmarking against commercial modelling packages using a pluvial and point‐source test case demonstrates that the scheme does not impede the ability of LISFLOOD‐FP to match both predicted depths and velocities of full shallow water models. The stability of the scheme in conditions unsuitable for traditional two‐dimensional hydraulic models is then demonstrated using a pluvial test case over a complex urban digital elevation model containing buildings. Deterministic single‐parameter sensitivity analyses undertaken using this test case show limited sensitivity of predicted water depths to both the chosen routing speed within a physically plausible range and values of the depth threshold parameter below 10 mm. Local instabilities can occur in the solution if the depth threshold is >10 mm, but such values are not required even when simulating extreme rainfall rates. The scheme yields a reduction in model runtime of ~25% due to the reduced number of cells for which the hydrodynamic equations have to be solved. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   
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Redshift surveys such as the Sloan Digital Sky Survey (SDSS) have given a very precise measurement of the galaxy luminosity function down to about   MR =−17 (≈ MB =−16)  . Fainter absolute magnitudes cannot be probed because of the flux limit required for spectroscopy. Wide-field surveys of nearby groups using mosaic CCDs on large telescopes are able to reach much fainter absolute magnitudes, about   MR =−10  . These diffuse, spiral-rich groups are thought to be typical environments for galaxies, so their luminosity functions should be the same as the field luminosity function. The luminosity function of the groups at the bright end  ( MR < −17)  is limited by Poisson statistics and is far less precise than that derived from redshift surveys. Here we combine the results of the SDSS and the surveys of nearby groups, and we supplement the results with studies of Local Group galaxies in order to determine the galaxy luminosity function over the entire range  −25 < MR < −9  . The average logarithmic slope of the field luminosity function between   MR =−19  and   MR =−9  is  α=−1.26  , although a single power law is a poor fit to the data over the entire magnitude range. We also determine the luminosity function of galaxy clusters and demonstrate that it is different from the field luminosity function at a high level of significance; there are many more dwarf galaxies in clusters than in the field, due to a rise in the cluster luminosity function of  α∼−1.6  between   MR =−17  and   MR =−14  .  相似文献   
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The frequency and intensity of hot weather events are expected to increase globally, threatening human health, especially among the elderly, poor, and chronically ill. Current literature indicates that emergency preparedness plans, heat health warning systems, and related interventions may not be reaching or supporting behavior change among those most vulnerable in heat events. Using a qualitative multiple case study design, we comprehensively examined practices of these populations to stay cool during hot weather (“cooling behaviors”) in four U.S. cities with documented racial/ethnic and socio-economic disparities and diverse heat preparedness strategies: Phoenix, Arizona; Detroit, Michigan; New York City, New York; and Philadelphia, Pennsylvania. Based on semi-structured in-depth interviews we conducted with 173 community members and organizational leaders during 2009–2010, we assessed why vulnerable populations do or do not participate in health-promoting behaviors at home or in their community during heat events, inquiring about perceptions of heat-related threats and vulnerability and the role of social support. While vulnerable populations often recognize heat's potential health threats, many overlook or disassociate from risk factors or rely on experiences living in or visiting warmer climates as a protective factor. Many adopt basic cooling behaviors, but unknowingly harmful behaviors such as improper use of fans and heating and cooling systems are also adopted. Decision-making related to commonly promoted behaviors such as air conditioner use and cooling center attendance is complex, and these resources are often inaccessible financially, physically, or culturally. Interviewees expressed how interpersonal, intergenerational relationships are generally but not always protective, where peer relationships are a valuable mechanism for facilitating cooling behaviors among the elderly during heat events. To prevent disparities in heat morbidity and mortality in an increasingly changing climate, we note the implications of local context, and we broadly inform heat preparedness plans, interventions, and messages by sharing the perspectives and words of community members representing vulnerable populations and leaders who work most closely with them.  相似文献   
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