Perturbation of gene expression and steroidogenesis with in vitro exposure of fathead minnow ovaries to ketoconazole     

Perkins EJ  Garcia-Reyero N  Villeneuve DL  Martinovic D  Brasfield SM  Blake LS  Brodin JD  Denslow ND  Ankley GT 《Marine environmental research》2008,66(1):113-115

Ketoconazole is a fungicidal drug that inhibits function of cytochrome P450s in the synthesis of steroids. To examine if inhibition of P450 function affects gene expression in a dynamic manner, we conducted in vitro exposures of ovary tissue from fathead minnows (Pimephales promelas) to 0.5 microM ketoconazole to investigate effects on steroid production and gene expression over time. Expression of four key steroidogenesis genes was examined at 1, 6, and 12h of exposure. 11 beta- and 20 beta-hydroxysteroid dehydrogenases were down regulated at 1h and Cytochrome P450 17 was down-regulated at 12h, consistent with the absence of steroid production. In contrast, cytochrome P450 19A was up-regulated at 6h, indicating feedback regulation. Microarray analysis of 12h exposures indicated enrichment of biological processes involved in neurotransmitter secretion, lymphocyte cell activation, sodium ion transport, and embryonic development. These data suggest that, with the exception of cytochrome P450 19A, these steroid metabolic genes are regulated in a feed forward manner and that the effects of ketoconazole may be broader than anticipated based on the mechanism of action alone.  相似文献   

Decentralized water supply by reservoir network reduces power demand for water distribution in a semi-arid basin     

Antônia Tatiana Pinheiro do Nascimento  Natália Holanda Maia Cavalcanti  Bruno Parente Leitão de Castro 《水文科学杂志》2019,64(1):80-91

In the Brazilian semi-arid region, thousands of small dams have been built over time to enhance water availability, accumulating water and hydraulic energy at high altitudes. Simulations were performed in this study to assess how the arrangement of reservoirs impacts on the power demand for water distribution in the Banabuiú River Basin (19?800 km²), Brazil. The power required to pump water from 1405 reservoirs to all districts with diffuse demands is 6.5 GWh/year, whereas in the scenario with only the 12 larger strategic reservoirs, the power demand reached 45.3 GWh/year. Alone, the largest reservoir in the basin can supply water to all districts. Nonetheless, in that scenario, the power demand would reach 195 GWh/year, which is 30 times the power required in the real reservoir arrangement. Thus, decentralization by small reservoirs not only promotes more democratic access to water, but also increases energy efficiency by storing it at higher altitudes and closer to the diffuse demands.  相似文献   

THE NEW PETROLOGY     

CHARLES P.BERKEY 《地质学报》1922,1(Z1):4-11

I suppose that one is not far wrong in saying that Petrology is thescience of rocks.But we have not made ourselves very clear by saying it.To the average student of the subject.who must judge from what he is taught,  相似文献