Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

Estimation of Sea Ice Thickness from SARAL/AltiKa in Drifting Orbit Phase     

Purvee Joshi  Sandip R. Oza  Ujjwal K. Gupta  Shailendra Saini  D. Ram Rajak  I. M. Bahuguna 《Marine Geodesy》2020,43(3):302-323

Abstract

Intra and inter-annual variations in the sea ice thickness are highly sensitive indicators of climatic variations undergoing in the earth’s atmosphere and oceans. This paper describes the method of estimating sea ice thickness using radar waveforms data acquired by SARAL/Altika mission during its drifting orbit phase from July 2016 onwards yielding spatially dense data coverage. Based on statistical analysis of return echoes, classification of the surface has been carried out in three different types, viz. floe, lead and mixed. Time delay correction methods were suitably selected and implemented to make corrections in altimetric range measurements and thereby freeboard. By assuming hydrostatic equilibrium, freeboard data were converted into sea ice thickness. Results show that sea ice thickness varies from 4 to 5?m near ice shelves and 1 to 2.5?m in the marginal sea ice regions. Freeboard and sea ice thickness estimates were also validated using NASA’s Operation Ice Bridge (OIB) datasets. Freeboard measurements show very high correlation (0.97) having RMSE of 0.13. Overestimation of approximately 1–2?m observed in the sea ice thickness, which could be attributed to distance between AltiKa footprint and OIB locations. Moreover, sensitivity analysis shows that snow depth and snow density over sea ice play crucial role in the estimation of sea ice thickness.  相似文献   

Occurrence of Nummulitic coralline limestone from offshore Palar Basin,Bay of Bengal,India     

S. Ramesh  K. Nvv Murthy  S. M. Hussain  S. Ramasamy  G. A. Ramadass 《Marine Georesources & Geotechnology》2020,38(1):57-63

Abstract

Palar basin is located between Pennar and Cauvery sedimentary basins of East coast of India in Bay of Bengal, northeast Indian Ocean. Sea floor drill (Wire-line Autonomous Coring System – WACS) with operational capability of up to 3000?m water depth was developed to collect long cores from deep sea floor for geotechnical and ocean resource assessment studies. During the drilling operation it encountered Nummulitic coralline limestone of Lower Eocene age at 18 meters below the seafloor (mbsf) at 850?m water depth indicating carbonated platform presence for the first time at the study region. Bathymetry contour from Naval Hydrography Chart and General Bathymetric Chart of the Oceans (GEBCO) has revealed the presence of shallow mounds from 50 to 200?m depth closure contour near the sampling site at 850?m water depth which might be a submerged carbonated structure. Since, Nummulites are shallow water dwelling fauna (<20?m depth) but its occurrence at 18 mbsf in 850?m water depth is recorded because of the advancement in technology tool for long core sampling by means of sea floor drill.  相似文献   

Recent changes in the population of juvenile Atlantic salmon in the Matamek River, Quebec, Canada     

Gibson  R. John 《ICES Journal of Marine Science》1978,38(2):201-207

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Geopotential topography of deep levels in the Pacific Ocean     

R. K. Reed 《Journal of Oceanography》1970,26(6):331-339

After examination of the baroclinic structure below 3000 db, recent data were used to map the geopotential topography at 1000, 1500, and 2000 db (referred to 3000 db) in the Pacific Ocean. In the high-latitude regions and in the western boundary currents, considerable relief is present at 1000 and 1500 db, and the Circumpolar Current is still well-developed at 2000 db. The geostrophic circulation inferred is very similar to the surface circulation in these areas. Neglect of the structure below 1000 db results in appreciable errors in estimates of the transport of these currents; direction of near-surface flow is properly shown, however, and computed speeds are not greatly in error. In the equatorial Pacific and central North Pacific, little relief is present, and the trends are generally unlike the surface flow.  相似文献   

Random dynamic analysis of multi-body offshore structures     

R.S. Langley 《Ocean Engineering》1984,11(4):381-401

A general method for the dynamic analysis of multi-body offshore structures is presented, being based on a constraint matrix approach. A method of deriving the constraint matrix for a general structure is given, and this is then used to derive the equations of motion of a whole system from those of it's component parts. The response of the system to both first and second order random wave forces is found and then used to calculate the forces and moments in the connecting mechanisms. The structure is assumed to have rigid component parts and a linearised frequency domain analysis method is used, although other methods are discussed. To illustrate it's use, the method is applied to both the SBS and Yoke-CALM design of offshore mooring terminal.  相似文献   

Meanders in the Antarctic Polar Frontal Zone and their impact on phytoplankton     

Mark R. Abbott  James G. Richman  Jasmine S. Nahorniak  Brett S. Barksdale 《Deep Sea Research Part II: Topical Studies in Oceanography》2001,48(19-20)

The Antarctic Polar Front is a complex set of meandering jets, which appear to support enhanced primary productivity. The US Joint Global Ocean Flux Study conducted a series of survey and process studies in part to study the processes regulating primary productivity in this high nutrient, low chlorophyll region. We deployed a set of surface velocity drifters, some of which were equipped with bio-optical sensors, to study the temporal and spatial scales of biological and physical processes in the Antarctic Polar Frontal Zone. There were two primary sets of deployments: November 1997 before the spring bloom and January 1998 after the spring bloom. The November deployment revealed a strong spring bloom that lasted about 10 days. In late spring, when incoming solar radiation began to increase, the vertical motions associated with the meanders strongly affected the accumulation of phytoplankton biomass, primarily through their impact on light availability. Weaker meandering was observed in the January deployment, and chlorophyll values remained relatively constant. As the bloom began to decay, it appears that nutrient availability became more important in regulating phytoplankton photosynthesis. Some of the drifters in the November deployment were deployed in coherent clusters, thus allowing us to calculate vertical velocities associated with the meanders. Estimates of fluorescence/chlorophyll suggest that areas of upwelling and downwelling alternately decrease and increase photosynthetic stress, perhaps as a result of changes in the availability of iron or light during the formation of the bloom.  相似文献   

Sedimentary environments and facies: H.G. Reading (editor). Blackwell Scientific Publications, Oxford, 1979, 557 pp., £13.00     

S.R. Aston 《Marine Geology》1981,40(3-4):273-274

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An experimental pressure compensated wet suit     

D.R. Burton  M.P. Norton   《Ocean Engineering》1982,9(3):281-290

Personal thermal insulation by means of neoprene from wet suits provides adequate temporary control of body heat loss only at the shallow end of the air diving depth range, but it constitutes by far the most popular approach to diver thermal support. Howewer, compression of the trapped gas phase in neoprene foam seriously reduces its insulation performance on exposure to high ambient pressure. With conventional wet suits equipped with heating, the necessary level of power required at depths greater than about 30 m is too high, and also unsafe without the back up that increased insulation would provide. One approach to the goal of depth-insensitive insulation is to use a wet suit with a continuous internal gas space pressurized nominally to ambient pressure, so that its thickness remains substantially constant at all depths. The composite material properties required are: outer skins that are tough, flexible and free of pin holes; an open foam internal structure capable of resisting, without significant dimensional change, the relatively small pressure changes that occur over the height of a man; and a high bond strength.Samples of a composite material that meets these requirements have been developed, and a prototype suit has been successfully fabricated by conventional techniques.  相似文献   

Seasonal and diurnal variations in atmospheric pressure     

V. V. Ivanov 《Izvestiya Atmospheric and Oceanic Physics》2007,43(3):323-337

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