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981.
982.
The measurement of enzymic activity in plankton communities is useful in the study of marine ecosystems. Such measurements can lead to a clearer understanding of the biological transformations in plankton communities at a particular time. However, the assays are somewhat time-consuming. To facilitate analysis of large numbers of samples, we have developed a method of quick-freezing of whole cells and of cell-free extracts of the diatom, Skeletonema costatum, followed by storage at ?60°C for up to one week. No loss in either the electron transport system (ETS) or the glutamate dehydrogenase (GDH) activity occurs. Similar conditions of storage can be used for the preservation of ETS activity in the marine copepod Calanus pacificus. Also, no measurable loss in either the GDH or the ETS activity is detectable after the quick-frozen whole cells of S. costatum have been kept frozen for over a year.  相似文献   
983.
984.
985.
Maps of the boundary topography for anaerobic waters in the Black Sea are plotted for the summers of the 1920s and 1980s. The mean depths of the H2S boundary for this period are determined. It is shown that the variation in the distribution and average location of the hydrogen sulphide zone has remained constant for the last 60 years.Translated by Mikhail M. Trufanov.  相似文献   
986.
The paper reports computed data on the seasonal variability of the Atlantic Ocean currents which were obtained through numerical integration (using conservative difference schemes) of non-linear equations of hydrothermodynamics taking into account an upper quasi-homogeneous layer and the bottom topography. The seasonal variability of the meridional heat transfer is discussed. The results were obtained using an adaptation approach.Translated by Vladimir A. Puchkin.  相似文献   
987.
988.
Juvenile coho salmon, Onchorynchus kisutch, were exposed to 95mTc in both saltwater and freshwater to study the biokinetic behavior of this element in an anadromous fish. In freshwater, equilibrium whole-body concentration factors (CF5) ranged from 28 to 32 in two separate experiments, in sharp contrast to an equilibriumCF of < 5 in saltwater. While the uptake kinetics were unimodal in saltwater, in freshwater, biomodal uptake kinetics were observed.Technetium-95m retention was biphasic in freshwater depuration experiments, an initial rapid loss of 21% to 26% of the steady-state activity with an elimination half-life of 3 to 5 days was followed by a slower loss rate, with a whole-body elimination half-life ranging from 23 to 33 days. In saltwater, retention was monophasic with an elimination half-life of 5–16 days. Dramatic decreases in 95mTc body burdens when freshwater-labeled fish are transferred to Tc-labeled saltwater indicate that the changes in physiology accompanying saltwater acclimation lead to the loss of 95mTc.  相似文献   
989.
990.
The biogeochemistry of organic matter in a macrotidal estuary, the Loire, France, has been studied for two years during different seasons. Both particulate matter and sediment have been sampled in the riverine zone, in the maximum turbidity zone and in the ocean near the river mouth. Two techniques have been used: carbon isotopic ratio determination and analysis of lipid-marker signatures in the n-alkane, n-alkene and fatty acid series. For the period corresponding to the output of the maximum turbidity zone in the ocean, the complete change of organic matter, continental in nature in the inner estuary, pure marine in the outer estuary is well illustrated by the decrease of δ13C values and of carbon preference index of n-alkanes. Input sources of organic matter by continental plants, plankton and micro-organisms are discussed from biogeochemical-marker analyses data along with the processes of accumulation of particles and their evolution with the season. Some criteria for evidencing the nature of various organic-matter pools are assessed and compared in different chemical-marker series as follows: high molecular weight n-alkanes and fatty acids, perylene for continental imprints, polyunsaturated 18-, 20- and 22-carbon fatty acids, n-C17, n-alkenes and squalene for algae imprints, branched iso and anteiso fatty acids, Δ11-C18:1 for microbial imprints.  相似文献   
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