Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach     

Miller KA  Assunção MG  Dangerfield NJ  Bandiera SM  Ross PS 《Marine environmental research》2005,60(2):153-169

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.  相似文献   

Seasonal variation in trawl codend selection of northern North Sea haddock   总被引：1，自引：0，他引：1  

Ozbilgin  H.; Ferro  R.S.T.; Robertson  J.H.B.; Holtrop  G.; Kynoch  R.J. 《ICES Journal of Marine Science》2006,63(4):737-748

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Summer distribution and geochemical composition of suspended-particulate matter in the east china sea     

Jia-Jang Hung  Chia-Lin Chan  Gong-Ching Gong 《Journal of Oceanography》2007,63(2):189-202

The distribution and geochemical composition of suspended-particulate matter (SPM) in the East China Sea (ECS) were investigated during the summer period of high continental runoff to elucidate SPM sources, distribution and cross-shelf transport. The spatial variability of SPM distribution (0.3–6.5 mg l⁻¹) and geochemical composition (POC, Al, Si, Fe, Mn, Ca, Mg and K) in the ECS was pronounced during summer when the continental fluxes of freshwater and terrestrial materials were highest during the year. Under the influences of Changjiang runoff, Kuroshio intrusion, surface production and bottom resuspension, the distribution generally showed strong gradients decreasing seaward for both biogenic and lithogenic materials. Particulate organic carbon was enriched in surface water (mean ∼18%) due to the influence of biological productivity, and was diluted by resuspended and/or laterally-transported materials in bottom water (mean 9.4%). The abundance of lithogenic elements (Al, Si, Fe, Mn) increased toward the bottom, and the distribution correlations were highly significant. Particulate CaCO₃ distribution provided evidence that the SPM of the bottom water in the northern part of the study area was likely mixed with sediments originally derived from Huanghe. A distinct benthic nepheloid layer (BNL) was present in all seaward transects of the ECS shelf. Sediment resuspension may be caused by tidal fluctuation and other forcing and be regarded as the principal agent in the formation of BNL. This BNL was likely responsible for the transport of biogenic and lithogenic particles across or along the ECS shelf. Total inventories of SPM, POC and PN are 46, 2.8 and 0.4 Tg, respectively, measured over the total area of 0.45 × 10⁶ km² of the ECS shelf. Their mean residence times are about 27, 13 and 11 days, respectively. The inventory of SPM in the water column was higher in the northernmost and southernmost transects and lower in the middle transects, reflecting the influences of terrestrial inputs from Changjiang and/or resuspended materials from Huanghe deposits in the north and perhaps from Minjiang and/or Taiwan’s rivers in the south. The distribution and transport patterns of SPM and geochemical elements strongly indicate that continental sources and cross-shelf transport modulate ECS particulate matter in summer.  相似文献   

Tank wall effects on broadside current forces on barge models     

S.K. Chakrabarti  D.C. Cotter 《Ocean Engineering》1994,21(5)

Three different barge sizes were tested in uniform current in a tank of limited width. One of the barges was tested with and without bilge keels. Tests were conducted in both deep and shallow water. The barges were placed broadside to the current flow and the lateral forces on the barges were measured. The effect of the tank walls on these forces is clearly shown.  相似文献   

Observations on the effect of tropical cyclones on gillnetting in the Bay of Bengal     

Pati  S. 《ICES Journal of Marine Science》1982,40(1):62-66

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Cross-shelf exchange in a model of the Ross Sea circulation and biogeochemistry   总被引：1，自引：0，他引：1  

Michael S. Dinniman  John M. Klinck  Walker O. Smith  Jr. 《Deep Sea Research Part II: Topical Studies in Oceanography》2003,50(22-26):3103

Transport of warm, nutrient-rich Circumpolar Deep Water (CDW) onto Antarctic continental shelves and coastal seas has important effects on physical and biological processes. The present study investigates the locations of this transport and its dynamics in the Ross Sea with a high-resolution three-dimensional numerical model. The model circulation is forced by daily wind stress along with heat and salt fluxes calculated from atmospheric climatologies by bulk formulae. All surface fluxes are modified by an imposed climatological ice cover. Waters under the Ross Ice Shelf are not included explicitly, but their effect on temperature and salinity is imposed in a buffer zone at the southern end of the model domain. A simple nutrient uptake is calculated based on the climatological chlorophyll distribution and Monod uptake kinetics.Model circulation is strongly affected by bottom topography, due to weak stratification, and agrees with schematics of the general flow and long-term current measurements except near the southern boundary. The sea-surface temperature is similar to satellite estimates except that the warmest simulated temperatures are slightly higher than observations. There is a significant correlation between the curvature of the shelf break and the transport across the shelf break. A momentum term balance shows that momentum advection helps to force flow across the shelf break in specific locations due to the curvature of the bathymetry (that is, the isobaths curve in front of the flow). For the model to create a strong intrusion of CDW onto the shelf, it appears two mechanisms are necessary. First, CDW is driven onto the shelf at least partially due to momentum advection and the curvature of the shelf break; then, the general circulation on the shelf takes the CDW into the interior.  相似文献   

Three cletodid copepods of the genera<Emphasis Type="Italic">limnocletodes</Emphasis> and<Emphasis Type="Italic">kollerua</Emphasis> (harpacticoida,cletodidae) from coastal marshes and estuaries in south Korea     

Ji Min Lee  Cheon Young Chang 《Ocean Science Journal》2007,42(4):255-267

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Design and projected performance of a flapping foil AUV   总被引：1，自引：0，他引：1  

Licht  S. Polidoro  V. Flores  M. Hover  F.S. Triantafyllou  M.S. 《Oceanic Engineering, IEEE Journal of》2004,29(3):786-794

The design and construction of a biomimetic flapping foil autonomous underwater vehicle is detailed. The vehicle was designed as a proof of concept for the use of oscillating foils as the sole source of motive power for a cruising and hovering underwater vehicle. Primary vehicle design requirements included scalability and flexibility in terms of the number and placement of foils, so as to maximize experimental functionality. This goal was met by designing an independent self-contained module to house each foil, requiring only direct current power and a connection to the vehicle's Ethernet local area network for operation. The results of tests on the foil modules in the Massachusetts Institute of Technology (MIT) Marine Hydrodynamics Water Tunnel and the MIT Ship Model Testing Tank are both used to demonstrate fundamental properties of flapping foils and to predict the performance of the specific vehicle design based on the limits of the actuators. The maximum speed of the vehicle is estimated based on the limitations of the specific actuator and is shown to be a strong function of the vehicle drag coefficient. When using four foils, the maximum speed increases from 1 m/s with a vehicle C/sub D/ of 1.4 to 2 m/s when C/sub D/=0.1, where C/sub D/ is based on vehicle frontal area. Finally, issues of vehicle control are considered, including the decoupling of speed and pitch control using pitch-biased maneuvering and the tradeoff between actuator bandwidth and authority during both the cruising and hovering operation.  相似文献   

Boussinesq-type modelling using an unstructured finite element technique     

Ole R. Srensen  Hemming A. Schffer  Lars S. Srensen 《Coastal Engineering》2004,50(4):181-198

A model for solving the two-dimensional enhanced Boussinesq equations is presented. The model equations are discretised in space using an unstructured finite element technique. The standard Galerkin method with mixed interpolation is applied. The time discretisation is performed using an explicit three-step Taylor–Galerkin method. The model is extended to the surf and swash zone by inclusion of wave breaking and a moving boundary at the shoreline. Breaking is treated by an existing surface roller model, but a new procedure for the detection of the roller thickness is devised. The model is verified using four test cases and the results are compared with experimental data and results from an existing finite difference Boussinesq model.  相似文献   

Second-order drift force response of offshore guyed towers     

R. S. Bisht  A. K. Jain 《Ocean Engineering》1995,22(3)

An iterative frequency domain method of analysis is presented for determining the response behaviour of Guyed Offshore Towers to low-frequency, second-order wave drift forces generated in a random sea environment. For the response analysis, the tower is idealized as a shear beam with a rotational spring at the bottom support. The guylines are replaced by a non-linear spring. The second-order drift force is considered to be proportional to the square of the wave elevation and is simulated using a drift force coefficient and the time history of a slowly varying wave envelope in random sea. The responses due to drift forces are obtained in frequency domain by incorporating the non-linearities produced due to non-linear guy lines. An example problem is solved under different random sea states to compare the response behaviour of the tower obtained by the second-order wave force, the first-order wave force and a combination of the two.  相似文献