一种灰树花菌丝体多糖GF4F1的结构特征及其抗肿瘤活性     

于广利  李春林  赵峡  杨晓华  宋乐天  高昊东 《中国海洋大学学报(自然科学版)》2006,36(6):915-918,974

以灰树花发酵菌丝体为原料，经过提取分离纯化得到1种水溶性多糖GF4F1，经高碘酸氧化、Smith降解、甲基化、红外光谱（IR）以及核磁共振波谱（NMR）等分析表明，该多糖是1种β-1, 3／1，6-葡聚糖，且小鼠体内实验结果表明其具有良好的抗肿瘤活性。在1mg／kg时，对小鼠抑制率为67.3％。  相似文献   

围垦区地下水位的控制及计算方法     

周理武  毛根海  宋建锋  陈观胜 《海洋学研究》2006,24(Z1):60-66

利用抽水井群和注水井群可以控制生态围垦区的地下水位.研究了潜水抽-注水井(群)耦合作用下浸润线的求解方法,利用势流理论和镜像原理,在假定含水层水平及土层渗透系数各向同性的条件下,推导出完整抽-注水井(群)耦合作用时浸润线的计算公式及非完整抽-注水井(群)耦合作用时浸润线的计算公式,还对潜水单个非完整抽水井及非完整抽-注水井群耦合作用时的解析解和数值解作了比较,结果表明,两者吻合得较好.同时还给出该理论用于地下水位控制中求解抽水量和注水量的方法等,对工程设计有实用价值.  相似文献   

使用简易测试平台检测马哈克扭力仪的性能     

田树川  宋协法  邱天霞 《海洋湖沼通报》2002,(3):71-75

本文描述简易测试平台的原理与制作，对马哈克MAIHAK MDS－820型扭力仪检测模拟负载的准确性进行探讨。  相似文献   

海底沉积物分析测试方法标准的研究   总被引：3，自引：0，他引：3  

夏宁  宋苏顷  刘昌岭  张红  林学辉 《海洋科学》2002,26(11):53-56

依据海底沉积物分析测试方法标准研究的总体设计，完成了40个分析项目的21份验证报告，进行了实验室间精密度协作性检验工作，在海底沉积物CaCo3,H2Os^-，H2O^ 的分析方法研究中取得新进展，并建立了ICP－AES法测定海底沉积物中常量、微量、稀土元素分析方法。  相似文献   

雄性三倍体长牡蛎繁殖潜力的初步研究   总被引：1，自引：0，他引：1  

丁君  张国范  常亚青  李霞  巩宁 《海洋科学》2001,25(9):55-57

国内外很多人做过三倍体长牡砺的诱导工作，但在三倍体性腺发育及配子活力方面的研究，目前仅见Ａｌｌｅｎ等１９８６年和１９９０年对三倍体的海螂和长蛎异常配子的发生报道。Ｃｏｘ１９９６年研究了悉尼岩牡蛎三倍体性腺发育情况，姜卫国等对三倍体合浦珠母贝的生殖腺进行了观察，李霞［１］对三倍体长牡蛎的性腺发育做了较系统的分析和总结。作者用流式细胞仪对长牡蛎Ｃｒａｓｓｏｓｔｒｅａ　ｇｉｇａｓ二、三倍体的性腺细胞进行了初步分析，回答了三倍体长牡蛎生殖潜力及性比方面的一些问题，为进一步研究三倍体长牡蛎的生长、繁殖、杂…  相似文献   

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

纳米碳酸钙材料的工业合成与应用     

姬泓巍  徐环  辛惠蓁  宁霞 《中国海洋大学学报(自然科学版)》2002,32(4):634-640

该文对目前工业上生产纳米碳酸钙的方法作了较为详细的综述。对一步碳化法、两步碳化法、多段喷雾碳化法、旋转填充床碳化反应器碳化技术制备链形、纺锤形、球形、立方形等不同形状的纳米碳酸钙材料的方法进行了比较和总结  相似文献   

三疣梭子蟹全人工工厂化育苗技术     

孙玉忠  王雪梅  宋全山  王玉华  王磊 《海洋湖沼通报》2002,(3):76-79

2000年笔者在日照市水产研究所利用1450m^3水进行梭子蟹全人工工厂化育苗生产，共出Ⅱ-Ⅲ期幼蟹512．7万只，创造了出苗量为3500只／m^3，总产值为150多万元的直接经济效益，同时对幼体培育的密度进行了初步试验，结果表明：从Z1至M期幼体培育密度10－15万／m^3为宜，M期以后密度以0．8－1．5万／m^3为宜。  相似文献   

Fatigue Behavior of Plain Concrete Under Biaxial Compression: Experiments and Theoretical Model     

ZHU  Jin-song 《中国海洋工程》2003,17(4):617-630

The effects of different lateral confinement stress on the fatigue behavior of and cumulative damage to plain concrete are investigated experimentally. Eighty 100 mm x 100 mm x 100 mm specimens of ordinary strength concrete are tested under constant- or variable-amplitude fatigue loading and lateral confinement pressure in two orthogonal directions. A fatigue equation is developed by modifying the classical Aas-Jakobsen S-N equation for taking into account the effect of the confined stress on fatigue strength of plain concrete. The results of variable-amplitude fatigue tests indicate that the linear damage theory proposed by Palmgren and Miner is unreasonable in the biaxial stress state. A nonlinear cumulative damage model that could model the effects of the magnitude and sequence of variable-amplitude fatigue loading and lateral confinement pressure is proposed on the basis of the evolution laws of the residual strains in the longitudinal direction during fatigue tests. The residual fatigue life predic  相似文献   

An Experimental Study of Several Special Issues Regarding the Ice Force on Conical Structures     

SHI  Qing-zeng 《中国海洋工程》2002,16(4):561-568

The effect of the cone spacing of a conieal structure on the ice force is studied by model experiments. The ice force reduction coefficient presented in this paper expresses the relationship between the ice force and the arrangement of cones. The experiments prove that the mode of the ice failure before the boundary of upward-downward bending cone (UDBC) is crushing. A conclusion can also be drawn from the experiments that the ice force on the boundary of UDBC is by far less than that on a vertical pile with the same diameter. Moreover, the ice force frequencies on upright and inve-rted cones are obtained under the field condition of the platform JZ20-2, respectively. The results show that the alterna-tion of the ice force on UDBC can hardly induce resonance of platform JZ20-2.  相似文献