Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

Nitrogen forms and decomposition of organic carbon in the southern Bohai Sea core sediments   总被引：5，自引：0，他引：5  

Song Jinming  Ma Hongbo  L&#; Xiaoxia 《海洋学报(英文版)》2002,21(1):125-133

Study on form characteristics of nitrogen in marine sediments is the primary method to research its biogeochemical cycling and nitrogen form characteristics in core sediments can reflect the process and results of early diagenesis in a certain degree. In this paper, Sequential extraction process in natural grain size was used for studying the existent forms of nitrogen in five core sediments of the southern Bohai Sea for the first time. Nitrogen was divided into two parts -transferable and fixed based on whether it could be extracted by the reagent. Distributions and early diagenesis of transferable nitrogen forms in the southern Bohai Sea were researched integratedly. Results indicate that IEF - N and OSF-N are predominant forms in transferable part in the studied core sediments. Contents of different nitrogen forms vary differently with depth, and have different diagenesis process. Decomposition constant of organic nitrogen (ON) and OC are about 15.51 × 10~(-3)a~(-1)and 4.79× 10~(-3)a~(-1) respective  相似文献   

大窑湾疏浚物倾倒区环境调查研究   总被引：2，自引：1，他引：2  

邵秘华  杨庆霄 《海洋湖沼通报》1991,(3):19-26

本文以1989年8月调查资料为基础,论述了大窑湾疏浚物倾倒区的水化学要素(pH、盐度、DO、COD、浊度)、营养盐(NO_2-N、NO_3-N、NH_4-N、PO_4-P、SiO_3-Si)和重金属(Cu、Zn、Pb、Cd)等污染物分布特征;分析了水质、底质的环境状况及潮流的影响。从环境地球化学观点探讨了该疏浚物倾倒区选择的科学性。  相似文献   

生物胺对锯缘青蟹大颚器发育的调节作用   总被引：1，自引：0，他引：1       下载免费PDF全文

叶海辉  李少菁  李祺福  黄辉洋  王桂忠 《海洋科学》2003,27(4):35-37

雌性锯缘青蟹(Scylla serrata)于实验的第1天、第5天、第10天，分别注射100μL剂量为1μg／g体质量的5-HT，OA和DA，第15天解剖。结果表明，5-HT对锯缘青蟹大颚器发育具有显的促进作用，OA和DA的作用不明显。经免疫细胞化学检测，大颚器内存在5-HT免疫阳性细胞，免疫阳性细胞多数为卵圆形，少数具有很长的胞突，该结果首次为5-HT参与大颚器的生理活动提供了形态学证据。  相似文献   

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis   总被引：3，自引：1，他引：3  

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2)

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po  相似文献   

海洋沉积物中的氮循环     

马红波  宋金明 《海洋科学集刊》2001,(43):96-107

氮(N)是生物生命活动的基本营养元素，N循环是整个生物圈物质和能量循环的重要组成部分。近几十年来，环境和资源问题的日益突出促进了人们对海洋生态系统的研究及对海洋资源的开发和利用，海洋中的N循环亦受到了广泛关注。尤其是20世纪90年代以来，随着国际地圈与生物圈计划(IGBP)、全球海洋通量联合研究(JGOFS)、全球海洋生态系统动力学研究(GLOBEC)等重大国际合作计划的实施，海洋中的N循环研究取得了重大进展。 本文主要对海洋沉积物中的N循环过程进行了阐述，包括海洋沉积物中N的生物地球化学循环(海洋中N与生物生产过程的关系、早期成岩作用及N的去营养化、沉积物中N循环及其控制机制、颗粒N的形成及其功能、N与其他生源要素循环的关系)及沉积物中N循环的研究方法等，以期对进一步开展N循环的研究有所帮助。  相似文献   

虾蟹类亲体生殖营养需求研究的进展   总被引：10，自引：0，他引：10  

艾春香  李少菁  王桂忠  林琼武 《台湾海峡》2003,22(2):254-261

本文综述了虾蟹类生殖营养的最新研究成果,并讨论了各营养素对虾蟹类亲体成熟、生殖和幼体质量的影响．此外,就今后在虾蟹类亲体生殖营养方面开展深入研究提出了一些建议。  相似文献   

非开挖城市地下信息管线数据采集和建库     

邵本科  马保松 《岩土钻凿工程》2008,(1):151-153

地下管线的数据采集和建库是实现地下管线信息化管理的基础和前提。本文提出了针对目前非开挖敷管数据三维数据采集和整理结合CAD进行三维成像的思路,与城市地下管线信息管理系统整合,实现地下管线的系统高效信息化管理。  相似文献   

Geochemical characteristics and genetic types of crude oils from the Tertiary system in the southern part of western Qaidam Basin, Northwes     

LI Hongbo ZHANG Min ZHANG Chunming PENG Dehua 《中国地球化学学报》2008,27(3)

  相似文献   

近海型含煤岩系沉积学及层序地层学研究进展   总被引：3，自引：1，他引：2       下载免费PDF全文

邵龙义  鲁静  汪浩  张鹏飞  韩德馨 《古地理学报》2008,10(6):561-570

层序地层学是近20年来发展起来的一门新的方法学科,并在聚煤作用分析中得到广泛应用。作者就近海型含煤岩系沉积学研究历史以及煤系层序地层学研究方法及有关问题进行探讨,认为近海环境的聚煤作用实际上是海平面(基准面)上升过程中发生的,同时提出煤层厚度受泥炭堆积速率与可容空间增加速率的控制：靠陆一侧冲积平原和三角洲平原沉积环境中,厚煤层主要出现在最大海泛面位置;而靠海一侧障壁-潟湖或碳酸盐岩台地沉积环境中,厚煤层主要出现在初始海泛面的位置;但就整个三级复合层序来说,层序中厚度最大、分布最广的煤层主要分布于可容空间增加速率最大的最大海泛面附近的位置。对于中国晚古生代近海型煤系中常见的“根土岩-煤-石灰岩”序列,聚煤作用发生于海相石灰岩“滞后时段”,即在海侵之后、海相石灰岩层真正沉积下来之前的时段,这一时段可容空间增加速率与泥炭堆积速率平衡,有利于聚煤作用发生。  相似文献