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31.
32.
Microbial degradation and utilization of proteins derived from bacterial detritus were investigated in a microcosm experiment
using Pseudomonas aeruginosa detritus as a substrate. To assess the effects of natural marine microbial communities on degradation and utilization of
protein derived from P. aeruginosa cells, four microcosms were prepared: natural seawater (containing the natural microbial community) with P. aeruginosa detritus (N+Pa), autoclaved seawater with P. aeruginosa detritus (A+Pa), natural seawater (N) and autoclaved seawater (A) without adding anything as a control. The numbers of total
and growing bacterial cells, protease activity, and transition of P. aeruginosa proteins were monitored in the four microcosms. Changes in the numbers of total and growing bacterial cells and protease
activities indicated that bacterial detritus significantly stimulated the microbial community in the microcosms. Both the
surviving P. aeruginosa in A+Pa and natural microbial community in N+Pa microcosms were able to degrade and utilize P. aeruginosa detritus; however, the community in N+Pa including various microbes maintained high activity longer, indicating that diversity
is an important factor in keeping the community active. Even under the very high protease activity in N+Pa, 39-kDa and 48-kDa
proteins from P. aeruginosa remained in the microcosm during the entire experiment (150 days). Immunoblotting suggested the 48-kDa protein was an intact
molecule of OprP, which had been detected from the dissolved fraction of natural seawater in previous studies. This result
suggests that the protein molecules that had been detected from natural seawater actually had a high tolerance to microbial
degradation. 相似文献
33.
对沙虫干抽提物成分的组成进行的研究结果表明 :游离氨基酸含量 w为 10 .3% ,占抽提物含氮物质的 77.9%。抽提物组成中 ,呈甘味的 Gly(3.2 % )、Ala(2 .5% )和呈鲜味的 IMP(0 .2 7% )、Glu(0 .2 5% )、琥珀酸 (0 .35% )等成分同沙虫干独特的风味有关 ,Na 、Cl-等无机离子也同其呈味相关。此外 ,牛磺酸含量 w高达 3.2 % ,是富含牛磺酸的一种海洋生物 ;精氨酸的含量 w亦较高 ,为 1.2 %。 相似文献
34.
Weekly aerosol samples were collected from March 1981 to June 1983 at the six stations in the western North Pacific region and analyzed for Ca and Na. By coupling data with those previously reported for Al (Tsunogai et al., 1985), the following results and conclusion have been obtained. There was a positive correlation between the atmospheric concentration of Al and the concentration of nonsea salt Ca (nssCa). The nssCa/Al ratios from the six stations, however, considerably varied (from 0.84±0.36 to 3.00±1.91), and the ratios were usally larger than those of the crustal average or of usual soil in Japan. The Ca/Al ratios of Asian desert soil and loess vary from 0.52 to 1.29, which are similar to the nssCa/Al ratios of aerosols in the surface air over the western North Pacific region except at Onna, Okinawa. The exception may be due to a local effect of coral. These results suggest that a large part of nonsea salt Ca in the surface air over the western North Pacific is derived from arid regions in Asia and that the nssCa/Al ratio in aerosol varies with that of the source material. 相似文献
35.
Noriyuki Suzuki 《Geochimica et cosmochimica acta》1984,48(2):243-249
A preliminary attempt to fractionate amorphous kerogens from terrigenous bulk kerogen by a benzene-water two phase partition method under acidic condition was made. Microscopic observation revealed that amorphous kerogens and structured kerogens were fractionated effectively by this method. Characteristics of the amorphous and structured kerogens fractionated by this method were examined by some chemical analyses and compared with those of the bulk kerogen and humic acid isolated from the same rock sample (Haizume Formation, Pleistocene, Japan). The elemental and infrared (IR) analyses showed that the amorphous kerogen fraction had the highest atomic ratio and the lowest atomic ratio and was the richest in aliphatic structures and carbonyl and carboxyl functional groups. Quantities of fatty acids from the saponification products of each geopolymer were in agreement with the results of elemental and IR analyses. Distribution of the fatty acids was suggestive that more animal lipids participate in the formation of amorphous kerogens because of the abundance of relatively lower molecular weight fatty acids (such as C16 and C18 acids) in saponification products of amorphous kerogens. On the other hand, although the amorphous kerogen fraction tends to be rich in aliphatic structures compared with bulk kerogen of the same rock samples, van Krevelen plots of elemental compositions of kerogens from the core samples (Nishiyama Oil Field, Tertiary, Japan) reveal that the amorphous kerogen fraction is not necessarily characterized by markedly high atomic ratio. This was attributed to the oxic environment of deposition and the abundance of biodegraded terrestrial amorphous organic matter in the amorphous kerogen fraction used in this work. 相似文献
36.
37.
The vertical section of microearthquakes, determined accurately by using the Hokkaido University network, shows two dipping zones (the double seismic zone) 25–30 km apart in the depth range of 80–150 km beneath the middle of Hokkaido in the southwestern side of the Kurile arc. Hypocentral distribution of large earthquakes (mb > 4) based on the ISC (International Seismological Centre) bulletin also shows the double seismic zone beneath the same region. The hypocentral distribution indicates that the frequency of events occurring in the lower zone is four times greater than that in the upper zone. The difference in seismic activity between the two zones beneath Hokkaido is in contrast with the region beneath northeastern Honshu in the northeastern Japan arc.Composite focal mechanisms of microearthquakes and individual mechanisms of large events mainly characterize the down-dip extension for the lower zone as is observed beneath northeastern Honshu. For the upper zone, however, the stress field is rather complex and not necessarily similar to that beneath northeastern Honshu. This may be considered to indicate the influence of slab contortion or transformation in the Hokkaido corner between the Kurile and the northeastern Japan arcs. 相似文献
38.
Shin-Ichi Machida Hisako Hirai Taro Kawamura Yoshitaka Yamamoto Takehiko Yagi 《Physics and Chemistry of Minerals》2007,34(1):31-35
High-pressure Raman studies of methane hydrate were performed using a diamond anvil cell in the pressure range of 0.1–86 GPa
at room temperature. Raman spectra of the methane molecules revealed that new softened intramolecular vibration mode of ν
1 appeared at 17 GPa and that the splitting of vibration mode of ν
3 occurred at 15 GPa. The appearance of these two modes indicates that an intermolecular attractive interaction increases between
the methane molecules and the host water molecules and between the neighboring methane molecules. These interactions might
result in the exceptional stability of a high-pressure structure, a filled ice Ih structure (FIIhS) for methane hydrate, up
to 40 GPa. At 40 GPa, a clear change in the slope of the Raman shift versus pressure occurred, and above 40 GPa the Raman
shift of the vibration modes increased monotonously up to 86 GPa. A previous XRD study showed that the FIIhS transformed into
another new high-pressure structure at 40 GPa. The change in the Raman spectra at 40 GPa may be induced by the transition
of the structure. 相似文献
39.
CHIME age dating of monazites from metamorphic rocks and granitic rocks of the Ryoke belt in the Iwakuni area, Southwest Japan 总被引:2,自引:0,他引:2
Abstract CHIME (chemical Th-U-total Pb isochron method) ages were determined for monazite from gneisses and granitoids of the Ryoke belt in the Iwakuni area. The CHIME monazite ages are 99.6 ± 2.4, 98.9 ± 2.1 and 98.2 ± 5.7 Ma for the Ryoke gneiss, 90.7 ± 2.2, 89.7 ± 2.0 and 89.3 ± 2.2 Ma for the Tajiri Granite, 91.0 ± 3.2, 90.6 ± 3.2 and 89.9 ± 3.2 Ma for the Namera Granite, 89.3 ±3.3 and 88.6 ± 5.6 Ma for a small stock at Shimizu, and 87.3 ± 1.6 and 86.6 ± 2.1 Ma for the post-tectonic Shimokuhara Granite. The CHIME monazite ages, interpreted as the time of the first attainment at the amphibolite facies conditions for the gneisses and as the time of emplacement for the granites, respectively, agree with the field intrusive sequence. The present dating documented that the Ryoke metamorphism in the Iwakuni area reached the amphibolite facies conditions at ∼98 Ma, was complete at -87 Ma, the time of emplacement of the post-tectonic Shimokuhara Granite. 相似文献
40.
Tadashi Kondo Hiroshi Sawamoto Akira Yoneda Manabu Kato Akihito Matsumuro Takehiko Yagi Takumi Kikegawa 《Pure and Applied Geophysics》1993,141(2-4):601-611
A new multi-anvil type high-presure apparatus has been developed using sintered diamond anvils to generate pressures over 30 GPa and temperatures up to about 2000°C. A maximum sample volume of about 1 mm3 is available in this system. The pressure was confirmed by dissociation of forsterite into Mg-perovskite and periclase. The basic techniques and problems in utilizing sintered diamond in the MA8 type high-pressure apparatus are discussed with an emphasis on the future prospect of incorporating simultancous X-ray diffraction observation. 相似文献