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111.
112.
Humic acid protein complexation 总被引:1,自引:0,他引:1
Interactions of purified Aldrich humic acid (PAHA) with lysozyme (LSZ) are investigated. In solution LSZ is moderately positively and PAHA negatively charged at the investigated pH values. The proton binding of PAHA and of LSZ is determined by potentiometric proton titrations at various KCl concentrations. It is also measured for two mixtures of PAHA-LSZ and compared with theoretically calculated proton binding assuming no mutual interaction. The charge adaptation due to PAHA-LSZ interaction is relatively small and only significant at low and high pH. Next to the proton binding, the mass ratio PAHA/LSZ at the iso-electric point (IEP) of the complex at given solution conditions is measured together with the pH using the Mütek particle charge detector. From the pH changes the charge adaptation due to the interaction can be found. Also these measurements show that the net charge adaptation is weak for PAHA-LSZ complexes at their IEP. PAHA/LSZ mass ratios in the complexes at the IEP are measured at pH 5 and 7. At pH 5 and 50 mmol/L KCl the charge of the complex is compensated for 30-40% by K+; at pH 7, where LSZ has a rather low positive charge, this is 45-55%. At pH 5 and 5 mmol/L KCl the PAHA/LSZ mass ratio at the IEP of the complex depends on the order of addition. When LSZ is added to PAHA about 25% K+ is included in the complex, but no K+ is incorporated when PAHA is added to LSZ. The flocculation behavior of the complexes is also different. After LSZ addition to PAHA slow precipitation occurs (6-24 h) in the IEP, but after addition of PAHA to LSZ no precipitation can be seen after 12 h. Clearly, PAHA/LSZ complexation and the colloidal stability of PAHA-LSZ aggregates depend on the order of addition. Some implications of the observed behavior are discussed. 相似文献
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On the relation between weather-related disaster impacts,vulnerability and climate change 总被引:1,自引:0,他引:1
Disasters such as floods, storms, heatwaves and droughts can have enormous implications for health, the environment and economic development. In this article, we address the question of how climate change might have influenced the impact of weather-related disasters. This relation is not straightforward, since disaster burden is not influenced by weather and climate events alone—other drivers are growth in population and wealth, and changes in vulnerability. We normalized disaster impacts, analyzed trends in the data and compared them with trends in extreme weather and climate events and vulnerability, following a 3 by 4 by 3 set-up, with three disaster burden categories, four regions and three extreme weather event categories. The trends in normalized disaster impacts show large differences between regions and weather event categories. Despite these variations, our overall conclusion is that the increasing exposure of people and economic assets is the major cause of increasing trends in disaster impacts. This holds for long-term trends in economic losses as well as the number of people affected. We also found similar, though more qualitative, results for the number of people killed; in all three cases, the role played by climate change cannot be excluded. Furthermore, we found that trends in historic vulnerability tend to be stable over time, despite adaptation measures taken by countries. Based on these findings, we derived disaster impact projections for the coming decades. We argue that projections beyond 2030 are too uncertain, not only due to unknown changes in vulnerability, but also due to increasing non-stationarities in normalization relations. 相似文献
116.
Willem P. Sijp Jan D. Zika Marc d’Orgeville Matthew H. England 《Climate Dynamics》2014,43(5-6):1661-1676
A stochastic analytical model of the Atlantic meridional overturning circulation (AMOC) is presented and tested against climate model data. AMOC stability is characterised by an underlying deterministic differential equation describing the evolution of the central state variable of the system, the average Atlantic salinity. Stability of an equilibrium implies that infinitesimal salinity perturbations are damped, and violation of this requirement yields a range of unoccupied salinity states. The range of states is accurately predicted by the analytical model for a coupled climate model of intermediate complexity. The introduction of climatic noise yields an equation describing the evolution of the probability density function of the state variable, and therefore the AMOC. Given the hysteresis behaviour of the steady AMOC versus surface freshwater forcing, the statistical model is able to describe the variability of the AMOC based on knowledge of the variability in the forcing. The method accurately describes the wandering between AMOC-On and AMOC-Off states in the climate model. The framework presented is a first step in relating the stability of the AMOC to more observable aspects of its behaviour, such as its transient response to variable forcing. 相似文献
117.
Alfred Stein Igor Staritsky Johan Bouma Jan Willem Van Groenigen 《International journal of geographical information science》2013,27(5):509-525
Abstract Geographical Information Systems can be used for processing spatial data to assess the risks of environmental contamination. Their use depends upon the amount and quality of available data, the models for interpolation and calculation of health risk, data processing procedures and interactivity. In this study, it is shown that interactive use of GIS is important for obtaining physically relevant results. Three forms of interactivity are distinguished: interactivity by means of user interfacing, interactivity requiring additional information outside GIS and interactivity when changing the use of GIS. Three stages in which interaction with GIS are crucial can be distinguished: application of geostatistics, choice of appropriate models, and decision making. This study focuses on three cases in The Netherlands dealing with soil contamination and soil stress analysis and with implications for risk assessment in which interactivity within GIS is analysed. Emphasis is given to contour volumes of polluted soil, to combine GIS with deterministic models and to apply land use scenarios. Finally, there is a discussion of how some forms of interactivity could be replaced by expert systems. 相似文献
118.
Xiaoying Yang David R. Steward Willem J. de Lange Simon Y. Lauwo Richard M. Chubb Eric A. Bernard 《International journal of geographical information science》2013,27(5):677-694
Development of a conceptualization of a hydrogeologic system serves as the basis of groundwater modeling. While existing groundwater data models are designed to store groundwater system information, none is designed to capture its conceptual view. This study addresses this need by presenting a new object-oriented Conceptualization Groundwater Data Model that represents a groundwater system as a series of aquifer layers with defined aquifer properties and water boundary conditions. A case study is presented that develops the conceptual view of the groundwater system beneath Konza Prairie. This single conceptualization is used to support groundwater models across existing technologies of finite difference, finite element, and analytical element methods. While the models each employ different mathematics, data input files, and formats, all models are founded on the same conceptualization process that is represented using this new data model. The case study illustrates the data model's promise as an effective mechanism for groundwater system conceptualization and data storage, and utility for various groundwater computational models. This conceptualization of a groundwater data model suggests a new focus on incorporating system conceptualization into data model design. 相似文献
119.
Tanya M. Doody Susan L. Gehrig R. Willem Vervoort Matthew J. Colloff Rebecca Doble 《水文研究》2021,35(7):e14291
Black Box (Eucalyptus largiflorens F. Muell.), is a keystone tree species of lowland semi-arid floodplain ecosystems in south-eastern Australia. E. largiflorens woodlands are of high conservation value and threatened by climate change-induced drought and irrigation water diversions due to their location on upper floodplain areas where flood frequency has declined. Water requirements of E. largiflorens have not been well quantified using empirical data. Accordingly, knowledge gaps exist in relation to volumes of environmental water required to maintain and improve ecological condition for disconnected floodplain woodlands. To further assist conservation and water resource management, we tested the use of drip irrigation to provide a variety of water regimes to experimental plots in order to monitor tree responses. Water was provided via irrigation delivery across four regimes representing known volumes of water, referred to as an environmental water provision, applied over a 22-week period for two Austral summers. Benefits to trees were identified by measuring transpiration and plant water status using sap flow sensors and a Scholander pressure chamber, respectively. Results indicate that volumes of 0.3, 0.4, 0.7 and 0.8 ML increased transpiration and improved plant water status in comparison to a control, with delivery recommended to commence early autumn. Greater volumes (1.4 ML), substantially increased transpiration and improved water status, especially when delivered at a rate of ~25 mm week−1 compared to a monthly 'burst' which broadly represented natural, sporadic summer rainfall in the region. For an environmental watering provision of 25 mm week−1, ~178 ha of E. largiflorens woodland can be watered with a 1 GL environmental water allocation. The study methods presented are relevant worldwide and our results further the collective understanding of the benefits environmental water provides to E. largiflorens. 相似文献
120.