藻红蛋白基因部分序列的克隆和顺序分析   总被引：3，自引：2，他引：1  

隋正红  张学成 《中国海洋大学学报(自然科学版)》1999,(1)

报道了真核红藻—龙须菜的藻红蛋白亚基基因的部分序列,位于β亚基的近碳端、α亚基的近氮端,包括β亚基部分３０９个核苷酸,α亚基部分１６２个核苷酸,还有间隔部分５５个核苷酸,可编码β近碳端的１０２个氨基酸和α近氮端的５４个氨基酸,并与目前已知的相应序列做了比较。该序列与已知红藻ＰＥ亚基基因相应部分的相似性为７７．９％到８１．１％。对应的氨基酸序列的相似性为７９．５％到８６．５％,蛋白质序列的保守性高于核苷酸序列。  相似文献   

3种不同来源的鲑鱼降钙素基因的克隆及其序列比较     

张宝红  茅云翔  王高歌  杨官品  张学成 《中国海洋大学学报(自然科学版)》2002,32(4):585-590

以 3种不同来源的鲑鱼基因组 DNA为模板 ,采用 PCR方法获得完整的 s CT基因。与Genbank中 Pink Salmon(Oncorhynchus gorbuscha)的降钙素基因序列进行比较 ,结果显示 :实验组的 Pink Salmon(O.gorbuscha)降钙素基因有 2个碱基差异 (第 39位 C→ A;第 5 1位 T→ C) ,氨基酸没有变化 ;Chum Salmon(O.keta)有 1个碱基的差异 (第 86位 G→ A) ,且引起 1个氨基酸的变化(第 2 9位 S→ N) ;而中国黑龙江产的鲑鱼 (Oncorhynchus sp.)降钙素基因序列与 Genbank中 PinkSalmon(O.gorbuscha)的降钙素序列完全相同 ,没有碱基差别。  相似文献   

Deep structure and lithospheric shear faults in the East Kunlun-Qiangtang region,northern Tibetan Plateau     

Xu  Zhiqin  Yang  Jingsui  Jiang  Mei  Li  Haibing  Xue  Guangqi  Yuan  Xuecheng  Qian  Hui 《中国科学:地球科学(英文版)》2001,44(1):1-9

An integrated study of earthquake seismic tomography in the Golmud-Tanggula Pass (west) and Gonghe-Yushu (east) with profiles traversing the East Kunlun-Qiangtang region of the northern Tibetan Plateau shows that the deep structure of the study region has the following characteristics: (1) from south to north the crustal thickness decreases from 70–75 km to 55–66 km, and the variation range of thickness (10 km) in the western part is smaller than that in the eastern part (20 km); (2) the crust has a sandwich-like structure and the middle crust has a lens-shaped low-velocity layer; (3) above 150 km in depth, the physical states of various terranes are marked by alternation of high-velocity and low-velocity bodies; and (4) the discontinuity of the lithospheric structure reveals the existence of three lithospheric shear faults on the the East Kunlun-Qiangtang region—the South Kunlun-A’nyêmaqên lithospheric shear fault, the Jinsha River lithospheric shear fault and the Xianshui River lithospheric shear fault. It is inferred that the easternward extrusion of northern Tibetan Plateau occurred in the lithospheric range.

  相似文献   

Deep structure and lithospheric shear faults in the East Kunlun-Qiangtang region,northern Tibetan Plateau   总被引：1，自引：0，他引：1  

Xu Zhiqin  Yang Jingsui  Jiang Mei  Li Haibing  Xue Guangqi  Yuan Xuecheng  Qian Hui 《中国科学D辑(英文版)》2001,44(1):1-6

The Cenozoic magmatic rocks of shoshonitic series in the eastern Qinghai-Tibet Plateau include potassic alkaline plutonic rocks, volcanic rocks, lamprophyres and acidic porphyries. Analytical results show that these different lithological rocks are extremely similar in Sr, Nd and Pb isotopic compositions with the range of 0.705 187– 0.707 254 for⁸⁷Sr/⁸⁶Sr, 0.512 305–0.512 630 for¹⁴³Nd/¹⁴⁴Nd, 18.53–18.97 for²⁰⁶Pb/²⁰⁴Pb, 15.51–15.72 for²⁰⁷Pb/²⁰⁴Pb and 38.38–39.24 for²⁰⁸Pb/²⁰⁴Pb. They are isotopically similar to the EMII end-member. This indicates that mantle metasomatism must have taken place in their source region. The formation of these particular rocks is related to crustal thinning and mantle upwelling in a large-scale strike-slip and pull-apart fault zone at about 40 Ma in northern and eastern Qinghai-Tibet Plateau  相似文献   

螺旋藻胞外和胞内核酸酶活性研究     

王高歌  杨官品  茅云翔  张宝红  张学成 《中国海洋大学学报(自然科学版)》2001,31(6):883-888

通过分析比较 2个螺旋藻物种 7个品系胞外和胞内核酸酶活性 ,确定了抑制胞外和胞内核酸酶活性的方法。用液体培养基清洗两次可消除胞外核酸酶活性 ,添加 EDTA(2 m mol L-1)可显著地抑制胞内核酸酶活性。高温 (6 5℃ )处理也能显著抑制胞内核酸酶活性  相似文献   

龙须菜四分孢子体cDNA文库的构建   总被引：1，自引：0，他引：1  

孙雪  张学成  隋正红  茅云翔 《中国海洋大学学报(自然科学版)》2003,33(5):727-732

为了研究不同世代和性别基因表达谱的差异 ,本文构建了龙须菜 (Gracilarialemaneiformis)四分孢子体的 c DNA文库。总 RNA提取使用 RNeasy Plant Mini Kit(Qiagen) ,c DNA文库构建使用 SMART c DNA Library Construction Kit(Clontech) ,包装蛋白购自 Promega公司。该c DNA文库含有 1 .2 8× 1 0 6个克隆 ,扩增文库的滴度是 1 .98× 1 0 9pfu/ m L,重组频率是 85.0 % ,插入片段几乎全部集中在 50 0～ 1 50 0 bp之间  相似文献   

Accumulative deformation in railway track induced by high-speed traffic loading of the trains   总被引：1，自引：0，他引：1  

Xuecheng Bian  Hongguang Jiang  Yunmin Chen 《地震工程与工程振动(英文版)》2010,9(3):319-326

Prediction and control of the permanent settlement of a track caused by traffic loading from trains is crucial to high-speed railway design and maintenance. In this study, a unified prediction model of accumulative deformation of geomaterials used in railway construction subjected to cyclic loadings is introduced and calibrated using physical model testing. Based on this versatile model, a calculation approach to determine the track structure settlement under repeated loadings caused by the movement of the wheel axle of the train is proposed. Regression analysis on the physical model testing is adopted to determine the parameters involved in the computational approach. Comparison of model test data and computed results shows that the parameters obtained from the back-analysis are consistent throughout the various testing conditions, and the proposed calculation approach is capable of satisfactorily predicting the accumulative settlement of the railway roadbed and subgrade soil for various axle loads and loading cycles. A case study of a high-speed railway is performed to demonstrate the feasibility of the proposed approach in realistic engineering applications. The computation results from the settlement development of a roadbed and subgrade soil are presented and discussed.  相似文献   

基于NDVI阈值法的森林冰冻受灾范围精确提取     

王学成  杨飞  高星  李丽 《地球信息科学学报》2017,19(4):549-558

快速提取森林冰雪受灾范围,有利于准确掌握森林受灾情况,为此类灾害性气候事件防灾减灾、森林资源管理和生态保护提供科学依据。本文利用2001-2007年NDVI数据,提取灾前植被NDVI参考值和正常波动范围,结合2008年NDVI数据提取冰雪冻灾范围。该方法弥补了基于单一时相的传统方法（NDVI差值法）忽略植被指数正常波动的问题,分像元提取植被NDVI正常波动范围,使提取结果更加客观合理。与传统方法提取结果对比,省级尺度的验证结果相同（即森林受灾率均为34.72%,而实地调查森林受灾率35.3%）,但2种方法在县市行政单元提取的森林受灾率相差较大。NDVI阈值法提取的森林冰雪受灾范围主要分布于湖南省南部地区,北部地区分布相对较少,而传统方法提取结果主要分布于湖南省北部地区,南部地区分布相对较少。根据实地考察资料显示,相比于传统方法,NDVI阈值法提取结果与实际森林冰雪冻灾空间分布信息更接近,精度更高,更适合于区域大尺度提取森林冰雪受灾范围。  相似文献   

再论无机生油假说及中国找油前景   总被引：2，自引：0，他引：2       下载免费PDF全文

袁学诚  林隆栋  许乐红 《中国地质》2017,44(3):473-484

新中国成立后石油勘探的第一个成功范例是1958年大庆油田的发现和开发。相应的概念上的创新是颠覆了只有海相地层才能找到大油田,确立陆相地层中同样可以找到大油田。普查勘探方法上的革新是采用了先进的地球物理方法开展战略性大面积普查,不再局限于几个地表有油苗的山前盆地进行工作。寻找石油资源急需开辟一个新的领域。其特点是普查勘探的对象不局限于地壳,还要拓宽到研究岩石圈。思想上的创新是要摒弃海相生油和陆相生油等陈旧生油理论,确立地幔生油的新思维。根据物理学热力学理论,甲烷是唯一一种在标准温压条件下稳定的碳氢化合物,直链烷属烃只有在压力3 MPa温度700°C时(相当于地下深度约100 km)才有可能形成。因此,海相生油理论和陆相生油理论都不靠谱,在地表赋存的不论是海相地层还是陆相地层内都不能生油,油气是地幔内(软流圈)无机生成,然后运移到海相盆地或陆相盆地中聚集成藏的。海相油田或陆相油田是储存的条件不同,但不是生成油田的机制不同。地球物理对寻找幔内生油区有独特的作用,许多石油地质学家认为,地球物理揭示的岩石圈内的低速低阻层与深藏油层有密切的关联。并且我们发现,在已开发的油田下方往往有软流圈物质的积聚。这些软流圈物质常常富有强烈的活动性,它们可以穿透覆盖在它们上面的地幔物质来到壳幔边界,在地幔中形成一个蘑菇云状的构造。这为开辟找石油的深层源头找到了捷径。根据中国地震层析资料探测得到的地幔内存在的低速带,以及区域地质背景条件,笔者提出中国3片找油最有远景的地区,即东亚西太平洋裂谷区、东特提斯地幔隆升区以及新疆深断裂分布地区:(1)东亚西太平洋低速带是在中新生代时,东亚大陆边缘岩石圈裂变所形成。它包括深浅、范围、形态各不相同的3个带。从西向东依次为:松辽—华北—东南沿海陆内裂谷带;日本海—黄海—东海—南海东亚边缘海裂谷带,以及四国海盆—帕里西维拉海盆—巴布亚新几内亚洋内裂谷带(2)东特提斯隆升区是指中国西南的兰坪盆地、思茅盆地、楚雄盆地为中心的一个地区,它们原本与当今最富油气的中东地区同属一个构造带,约在5 Ma时由于印度板块的向北挺进,青藏高原隆升,在这一地区的构造受到前所未有的破坏,但仍有残余下来可能含油气地区。(3)新疆地区由于受到印度地块的挤压,形成一系列背型和向型构造。深藏油气只能从切割它们的深大断裂中溢流到地表。笔者根据卫星重力资料,提出巴彦敖包—西宁不连续带;淖毛湖—茫崖不连续带;于田—克里雅河不连续带和阿拉木图—塔什库尔干不连续带等4条不连续带。它们都可能是深层石油上升的通道,希望能引起找油专家们关注。  相似文献   

Lyase Activities of Heterologous CpcS and CpcT for Phycocyanin Holo-β-subunit from Arthrospira platensis in Escherichia coli     

Junjie Yi  Di Xu  Xiaonan Zang  Dingyang Yuan  Bingran Zhao  Li Tang  Yanning Tan  Xuecheng Zhang 《中国海洋大学学报(英文版)》2014,13(3):497-502

Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.  相似文献