The young thalli of Porphyra yezoensis and P. haitanensis are induced by N-Methy-N‘-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investigated in all control groups,while in the induced groups, it is easy to find mutant cells or mutant cell masses in the thallus.The pigmentation mutants may be the result of NG inducement. The mutant rate increases obviously with the mutagen concentration or by prolonging the induction time. Within the scope of experiment, the inducement effects of Porphyra yezoensis are raised from 11.2% to 28.7%, and those of P. haitanensis from 10.1% to 20.2%. According to our experiments, the amount of mutant cells in the same area does not increase with the induction intensity The amount of mutant cells in every group of inducement of both species of Porphyra changes very little with the fixed fields check method. The lowest dose (25μg/mL) of this experiment has a high mutant rate. The mutant rate shows the percentage of mutant cells in all the survived cells, while the amount of mutant cells reflects the effect of inducement. Thus the optimum induction concentration of NG is 25μg/mL, and the optimum induction time is 30minutes. 相似文献
Climate change is one of the main factors that affect runoff changes. In the upstream of Minjiang River, the temperature increased significantly in the last 50 years, while the precipitation decreased on the contrary. In order to analyze the effect of climate change on site runoff, watershed runoff depth and evaporation, nine climate scenarios are assumed based on rainfall and temperature indicators. A SWAT model of Minjiang River is constructed, and runoff simulation is carried out with the nine scenarios. The results show that if precipitation increases or decreases 20 %, the change rate of runoff depth will increase or decrease 28–32 %; if temperature increases or decreases 2 °C, the change rate of runoff depth will decrease or increase 1–6 %; if temperature increases or decreases 2 °C, the change rate of the potential evaporation will increase or decrease 5–16 %, and the actual evaporation rate of variation will increase or decrease 1–6 %. Overall, precipitation variation has greater effect on simulated runoff than temperature variation dose. In addition, temperature variation has more obvious effect on the runoff simulation results in dry years than in wet years. The actual evaporation of watershed depends on evaporation capacity and precipitation and increases with the increasing of the potential evaporation and precipitation. The study also shows that the climate change scenarios analysis technology, combined with SWAT hydrological model, can effectively simulate the effect of climate change on runoff.
In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, the gfp reporter gene was inserted in the isiAB locus of Synechococcus sp.PCC7942 in different directions, and the GFP expression levels and the growth of the transgenic algae were compared. It was showed that the gfp gene could express in each direction, and no significant difference was detected on algal growth and GFP expression levels between the two recombinant algae. 相似文献
To investigate the influences of reservoir water level drawdown on slope stability, a series of numerical simulations were carried out to examine the impacts of slope angle, slope permeability and water drawdown speed on the pore water pressure (PWP) change during seepage, as well as the factor of safety (Fs) of the slope. Based on the numerical results, an estimation model relating the factor of safety to the influential factors mentioned above was developed. The model expression indicates that there are interaction effects between the slope angle, the drawdown speed and the slope permeability, with regard to the influences on slope stability. Furthermore, in view of the uncertainty characteristics of the slope permeability, reliability assessment was performed to examine the influences of those key parameters mentioned above on the probability of slope failure. 相似文献
