Colonization and Probiotic Effect of Metschnikowia sp. C14 in the Intestine of Juvenile Sea Cucumber,Apostichopus japonicus     

LI Ming  BAO Pengyun  SONG Jian  DING Jianfeng  LIU Yubin  MA Yuexin 《中国海洋大学学报(英文版)》2020,19(1):225-231

Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the control diet for 38 days or the C14-supplemented diet at 105 cells g−1 diet for 28 days,then the control diet from day 29 to day 38.The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28,and decreased from day 29 to day 38.Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control.Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38,respectively.In addition,there was an obvious enhancement in lysozyme activity(from day 21 to day 38 or day 33),phenoloxidase activity(from day 21 to day 28)and total nitric oxide synthase activity(from day 14 to day 38)in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control.There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine,lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber.These data suggested that the number of C14 cells should be maintained at 105 cfu(colony-forming units)g−1 intestine material for the maximum benefit.  相似文献   

湘中盆地西部构造变形的运动学特征及成因机制   总被引：6，自引：0，他引：6  

柏道远  贾宝华  王先辉  彭云益  贾朋远  凌跃新 《地质学报》2013,87(12):1791-1802

湘中盆地中部的大乘山 龙山EW向隆起将湘中盆地分为涟源和邵阳两个次级凹陷。湘中盆地西部上古生界盖层中主体构造为NE—NNE向褶皱和逆断裂,前人认为其变形与向北西的逆冲推覆有关。本文对湘中盆地西部进行了多条构造剖面调查,结果表明：涟源凹陷西部上古生界褶皱轴面大多直立、部分斜歪;走向逆断裂及斜歪褶皱轴面大多倾向NW,少量倾向SE。大乘山背斜为一轴面倾向NWW的倒转背斜,背斜核部及两翼发育大量以NWW倾向为主的逆断裂,背斜内长安组劈理均倾向NWW。邵阳凹陷西部上古生界中走向逆断裂和倒转与斜歪褶皱轴面总体倾向NWW,局部反冲构造带断裂倾向SEE。湘中盆地盖层变形主要受控于盖层底部不整合界面及石炭纪测水组煤系地层的滑脱,部分断裂切入加里东褶皱基底。上述各次级构造单元变形特征表明湘中盆地西部逆冲推覆的总体方向为SE,而不是前人所认为的NW。研究表明湘中盆地NE—NNE向褶皱和逆断裂形成于中三叠世晚期的印支运动和中侏罗世晚期的早燕山运动。分析认为湘中盆地西部向SE逆冲,与雪峰造山带东缘向SE逆冲及城步 新化岩石圈断裂向NW俯冲有关。  相似文献   

深穿透地球化学勘查技术对隐伏稀有金属矿的勘查指示：以甲基卡X03号锂矿脉为例     

鲁岳鑫  张必敏  刘汉粮  迟清华  窦备  刘福田  王强  谢明君  呼延钰莹 《地质学报》2023,97(1):291-305

花岗伟晶岩型锂矿是锂资源的重要矿产类型,目前随着勘查程度的提高,发现地表出露的伟晶岩型锂矿资源的几率越来越小,下一步的勘查重点应放到找寻隐伏型锂矿资源。近年来,深穿透地球化学勘查手段在隐伏矿勘查中发挥了重要作用。其中土壤微细粒分离测量技术能有效探测深部异常信息,已在多种景观覆盖区进行了试验,并取得了显著的效果。本文以四川甲基卡X03号隐伏锂矿脉为研究对象,利用土壤微细粒分离测量技术,开展深穿透地球化学勘查技术对隐伏锂矿脉探测的试验研究,并分析了成矿及伴生元素组合特征,结果表明：Li、Be、Rb、Cs、Sn、Nb、Ta、Bi、Pb、U等元素具有较好的相关性,富集程度高,异常明显,异常峰值显著;其中,Li-Be-Bi-Cs-Rb-Pb-U元素组合与研究区X03号矿脉成矿元素组合具有良好的耦合性;利用土壤微细粒分离测量技术取得的元素高异常范围与已知隐伏矿体具有较好的对应关系,可以将上述元素作为该区域寻找锂矿的指示元素。以上结果证实了深穿透地球化学勘查技术能有效指示深部隐伏锂矿体,可以将深穿透地球化学勘查技术应用于西部高寒山区景观覆盖区地球化学找矿勘查中。  相似文献   

CIE标准照明体D65光谱敏感性质的研究     

束越新  张萧  华玉生 《中国海洋大学学报(自然科学版)》2001,31(3):382-394

为寻求评价日光模拟器的新方法 ,作者基于异谱同色指数和显色指数分析了 D65光谱的敏感性质 ,并讨论了其间的差异。基于异谱同色指数分析了 D65光谱上特殊波长间敏感性质的关联性 ,以期更全面、准确地了解光谱的敏感性质  相似文献   

GIS空间数据安全管理技术方法研究     

贾培宏  史照良  龚越新 《现代测绘》2003,26(4):9-11

本文以课题“基础地理信息数据产品数字水印技术研究”为基础，研究出一套在GIS空间数据中隐藏标识数据生产单位版权以及用户使用权属的数字水印并对其进行压缩、加密的GIS空间数据安全管理技术方法，并从实际出发，设计了相应的应用程序技术方案，并给出了部分软件实例验证。这一技术方法的研究对于GIS空间数据的安全管理和安全共享都有很大意义，也是对数字水印应用新领域的探索。  相似文献   

姚安6.5级地震的震害特征     

樊跃新  非明伦  崔建文  包一峰  吕明 《地震研究》2002,25(3):302-308

对姚安M6．5地震极震区（Ⅷ度）及姚安，大姚，南华和牟定等县城内的建筑物与结构物的震害做了系统的调查。作为系统研究这一，叙述了多层钢筋混凝土框架，多层砌体，底层框架，砖木结构，土木结构各类房层，单层厂房及砖砌塔的地震破坏现象，分析了建筑物的地震震害特征及对建筑物震害加剧的主要影响，讨论了地震区域城镇建设中防震减灾应重视的问题，以及经验和教训。这对今后多震区的城市规划，建筑物设计具有一定的参考价值。  相似文献   

利用山西省资源优势发展环保型有机无机复肥   总被引：1，自引：0，他引：1  

王曰鑫  杨建荣  铁梅  贾明 《华北地质矿产杂志》1999,(2)

山西省有三分之二的中低产田土壤急需改良,研制和扩大环保型有机无机复肥是山西农业可持续发展的需要。利用山西的几种可改良土壤的矿物原料和有机废料与无机化肥配合试制出的“长效肥力宝”是一种新的环保型有机无机矿物复混肥。经初步试验,结果表明其肥效优于常规化肥的增产效果,土壤的有机质和N、P、K等养分含量有明显的提高。发展该类产品既保护了环境,又促进了农业生产的发展。  相似文献   

Characterization of Bacterial Community Associated with Four Organs of the Yesso Scallop (Patinopecten yessoensis) by High-Throughput Sequencing     

MA Yuexin  LI Ming  SUN Jingxian  HAO Zhenlin  LIANG Jun  ZHAO Xuewei 《中国海洋大学学报(英文版)》2019,(2):493-500

We used Illumina high-throughput sequencing of PCR-amplified V3-V4 16 S rRNA gene regions to characterize bacterial communities associated with the adductor muscles, gills, gonads and intestines of the Yesso scallop(Patinopecten yessoensis) from waters around Zhangzidao, Dalian, China. Overall, 421,276 optimized reads were classified as 25 described bacterial phyla and 308 genera. Firmicutes, Proteobacteria, Tenericutes, Bacteroidetes, Chlamydiae and Spirochaetae accounted for > 97% of the total reads in the four organs. The bacterial 16 S rDNA sequences assigned to Firmicutes and Proteobacteria were abundant in the adductor muscles, gills and gonads; while reads from Tenericutes were dominant in the intestines, followed by those from Firmicutes, Chlamydiae, Proteobacteria and Bacteroidetes. At the genus level, the dominant genera in the adductor muscles, gills and gonads appeared to be Bacillus, Enterococcus and Lactococcus, whereas Mycoplasma was dominant in the intestines. The relative abundances of Bacillus, Enterococcus, Lactococcus, Alkaliphilus, Raoultella, Paenibacillus and Oceanobacillus were significantly lower in the intestine than in the other three organs. Cluster analysis and principal coordinates analysis of the operational taxonomy units profile revealed significant differences in the bacterial community structure between the intestine and the other three organs. Taken together, these results suggest that scallops have intestine-specific bacterial communities and the adductor muscles, gills and gonads harbor similar communities. The difference in the bacterial community between organs may relate to unique habitats, surroundings, diet and their respective physiological functions.  相似文献